Evaluation of Antiseizure Activity of Essential Oil from Roots of Angelica archangelica Linn. in Mice.

In the present study, the effect of essential oil of the root of Angelica archangelica Linn. was evaluated against electrically and chemically induced seizures. The seizures were induced in mice by maximal electroshock and pentylenetetrazol. The effect of essential oil of the root of Angelica archangelica on seizures was compared with standard anticonvulsant agents, phenytoin and diazepam. The essential oil of the root of Angelica archangelica suppressed duration of tonic convulsions and showed recovery in maximal electroshock induced seizures while it delayed time of onset of clonic convulsions and showed mortality protection in pentylenetetrazol induced seizures. The essential oil of the root of Angelica archangelica also produced motor impairment at the antiseizure doses. The study indicated that the essential oil exhibited antiseizure effect. The antiseizure effect may be attributed to the presence of terpenes in the essential oil.

A possible participation of gonadotropin-releasing hormone in the neuroleptic and cataleptic effect of haloperidol.

Haloperidol, an antipsychotic agent, stimulates the release of gonadotropin-releasing hormone (GnRH), and this hormone is known to mimic some of the behavioral effects of haloperidol. Hence, the present study was carried out to find out the contribution of GnRH in the behavioral effects of haloperidol. The studies revealed that haloperidol (0.15, 0.25 and 0.5 mg/kg, i.p.) and leuprolide (GnRH agonist; 50, 100, 200 and 400 microg/kg, s.c.) dose-dependently inhibited conditioned avoidance response (CAR) in male Sprague-Dawley rats. In higher doses, haloperidol (0.5, 1 mg/kg, i.p.) and leuprolide (200, 400 microg/kg, s.c.) produced catalepsy in rats. Co-administration of sub-effective dose of leuprolide (50 or 100 microg/kg, s.c.) and haloperidol (0.15 or 0.5 mg/kg, i.p.) similarly inhibited CAR and induced catalepsy. Pre-treatment of rats with antide (GnRH antagonist; 10 microg/rat, s.c.), attenuated the inhibitory effect of both the agents on CAR; blocked leuprolide-induced catalepsy; and attenuated the intensity and reduced the duration of haloperidol-induced catalepsy. In conclusion, the studies suggest a possible role of GnRH in the neuroleptic and cataleptic effect of haloperidol.

Leuprolide--a GnRH agonist--prevents restraint stress-induced immunosuppression via sex steroid-independent peripheral mechanism in mice.

Gonadotropin-releasing hormone (GnRH) and sex steroids are known to modulate the immune system. To find out whether GnRH analogue can be useful to prevent the stress-induced immunosuppression, mice were treated with leuprolide (50 microg/mouse, s.c.) 30 min prior to restraint stress and immunological parameters were studied. Leuprolide prevented stress-induced decrease in relative weights of thymus, total leukocyte count and sheep red blood cells challenged humoral and cell-mediated immune reaction. Prior administration of GnRH antagonist (10 ng/mouse) through i.c.v. route did not alter the immunological effects of leuprolide. Further, the observed effect of leuprolide remained unaffected in castrated and ovariectomized mice. Thus, the investigations revealed that leuprolide prevented stress-induced immunosuppression through a peripheral mechanism, which is independent of systemic sex steroids.

Rapid and Simple RPHPLC Method for the Estimation of Metformin in Rat Plasma.

A simple reverse phase high-performance liquid chromatographic method has been developed for determining the concentration of metformin in rat plasma. The method employs C(18) column (300 mm x 2.4 mm i.d.), ammonium acetate (0.15 M) and acetonitrile (90:10; pH-5.5; 1.0 ml/min) as mobile phase and ultraviolet detection at 236 nm. Acetonitrile was used to simultaneously deproteinize rat plasma and extract metformin. The assay was linear in the concentration range of 0.33 mug-16.6 mug/ml with co-efficient of correlation 0.994. The retention time was 4.7 min. The method was found to be precise (% CV < 15%), accurate and suitable for pharmacokinetic study of orally administered metformin in rats.