ABSTRACT
OBJECTIVE: The aim of this study was to investigate the association between IL-1ß and IL-1α isoforms with chronic periodontitis in two Kenyan ethnic groups, Taitas and Swahilis. METHODS: A case-control study in which participants were assessed for dental plaque, gingival inflammation, pocket depth and gingival recession after informed consent. Buccal swab samples were obtained and deoxyribonucleic acid was isolated from the swabs using QIAamp DNA purification protocol followed by polymerase chain reaction amplification using specific primers to IL-1 α rs1800587 (-889) and rs17561 (+4845) and IL-1ß (rs16944 (-511) and rs11443624 (+3954). Restriction fragment length polymorphisms were recorded and association with clinical data was assessed. RESULTS: Three hundred and ninety participants were recruited; four loci (-511, -889, +3953 and +4845) were analyzed per subject, equivalent to 1560 analysis events. No deviation from Hardy Weinberg equilibrium 1df was observed. Frequency of allele 2 at IL-1ß +3954 was associated with chronic periodontitis in Taitas (OR = 1.94, 95% CI = 1.01 - 3.70, p = 0.045), whereas frequency of allele 1 at IL-1α -889 was associated with chronic periodontitis in Swahilis (OR = 3.16, 95% CI = 1.644 - 6.083, p less than 0.001). Allele 1 at locus IL-1α -889 was also associated with mild, (OR = 5.2, 95% CI = 1.445 - 18.71, p = 0.005), moderate (OR = 4.51, 95% CI = 2.08 - 9.79, p less than 0.001) and severe disease (OR = 2.19, 95% CI = 1.013 - 4.738, p = 0.042) in Swahilis. Haplotype 3 (allele 1 at all four loci) was significantly associated with chronic periodontitis in Taitas (OR = 2.4, 95% CI = 1.1 - 5.14, p = 0.022) and Swahilis (OR = 4.2, 95%CI = 1.35 - 13.3, p = 0.008). CONCLUSIONS: This study has shown that in the African population of Bantu origin, the two polymorphisms associated with chronic periodontitis are IL-1ß +3954 in Taitas and IL-1α-889 in Swahilis. Additionally, haplotype 3 was associated with chronic periodontitis in both ethnic groups.
ABSTRACT
The study aimed to describe the effect of an oral health education intervention on oral hygiene status and gingival inflammation among persons with HIV attending two comprehensive healthcare centres in Nairobi, Kenya. This was a quasi-experimental study of 195 participants (with 102 in the intervention group, and 93 serving as the control group) who were selected using stratified random sampling. The data were collected at baseline, at three months (review 1), and at six months (review 2) using an interviewer-administered World Health Organization clinical examination form. The prevalence of plaque among the participants in the intervention decreased from 70.6% to 18.6%, with a significant decrease in their mean plaque score, from 0.89 to 0.15. The prevalence of gingival inflammation in the intervention group decreased from 58.2% to 12.7%, with a significant decrease in the mean gingival score, from 0.66 to 0.11. No significant change in degree of oral hygiene and gingival inflammation was observed among the non-intervention group. There was a strong association between the change in the mean gingival score and the change in the mean plaque score between baseline and at six months for the intervention group. The regression analysis yielded a coefficient of determination (r2) of 0.76; therefore, 76% of the variation in change in gingival score was explained by the variables in the equation. Only the change in mean plaque score was a significant predictor of the change in gingival score.
