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Waqar, S N; Khan, H; Mekan, S F; Kayani, N; Raja, A J.

J Pak Med Assoc ; 54(10): 531-3, 2004 Oct.

Article in English | MEDLINE | ID: mdl-15552291

Subject(s)

Breast Neoplasms/diagnosis , Lymphangioma, Cystic/diagnosis , Adult , Breast Neoplasms/pathology , Breast Neoplasms/surgery , Diagnosis, Differential , Female , Humans , Lymphangioma, Cystic/pathology , Lymphangioma, Cystic/surgery

Banti's syndrome: case report and review of literature.

Waqar, S N; Jindani, S; Baig, N S; Waqar, M A; Ismail, F W; Tariq, M.

J Pak Med Assoc ; 54(2): 99-101, 2004 Feb.

Article in English | MEDLINE | ID: mdl-15134212

Subject(s)

Hypertension, Portal , Pancytopenia , Splenomegaly/diagnostic imaging , Adult , Female , Humans , Syndrome , Tomography, X-Ray Computed

An enzymatic method for the detection of human serum albumin.

Javed, M U; Waqar, S N.

Exp Mol Med ; 33(2): 103-5, 2001 Jun 30.

Article in English | MEDLINE | ID: mdl-11460880

ABSTRACT

Albumin is the most abundant protein in human serum. A dye-binding method is commonly used in clinical laboratories for its estimation using different types of dyes. However, all these dye methods were interfered by a variety of compounds. Here we present a method for the detection of albumin in human serum and other biological fluids. The principle is based on the fact that lactate dehydrogenase isoenzyme-5 (LDH-5) binds specifically to Dextran-Blue (DB). Albumin inhibits the binding of LDH-5 with DB. Absence of LDH activity in DB fraction after gel filtration indicates the presence of albumin in sample and vice versa.

Subject(s)

Chemistry, Clinical/methods , Serum Albumin/analysis , Chromatography, Gel , Humans , Isoenzymes/metabolism , L-Lactate Dehydrogenase/metabolism , Lactate Dehydrogenase 5 , Protein Binding , Sepharose/chemistry

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