Subject(s)
Bacteriological Techniques , Gram-Negative Bacteria/isolation & purification , Water Microbiology , Acetates/metabolism , Acinetobacter/isolation & purification , Culture Media , Cytophaga/isolation & purification , Fermentation , Flagella/cytology , Flavobacterium/isolation & purification , Glucose/metabolism , Gram-Negative Bacteria/classification , Gram-Negative Bacteria/metabolism , Indicators and Reagents , Oxidation-Reduction , Pseudomonas/isolation & purification , Reagent Kits, Diagnostic , Staining and LabelingABSTRACT
The influence of pH, application technique, and chlorine-to-nitrogen weight ratio on the bactericidal activity of inorganic chloramine compounds was determined with stock and environmental strains of Escherichia coli, Salmonella spp., Pseudomonas aeruginosa, Klebsiella pneumoniae, and Enterobacter cloacae. The rate of inactivation increased from 1.5 to 2 times as the chlorine-to-nitrogen weight ratio was adjusted from 2:1 to 5:1, 5 to 6 times as the pH was decreased from 8 to 6, and 5 to 6 times as the concentration was increased from 1 to 5 mg/liter. Separate additions of free chlorine and ammonia (concurrent addition and preammoniation) into seeded water at or below pH 7.5 resulted in killing comparable to that observed with free chlorine (99% inactivation in less than 20 s). At pH 8, inactivation by separate additions was considerably slower and was comparable to that by prereacted chloramine compounds (99% inactivation in 25 to 26 min). Determination of the effectiveness of inorganic chloramine compounds as primary disinfectants for drinking water must consider the method of application, pH and concentrations of chlorine and ammonia.