Suspect and non-target screening of contaminants of emerging concern in streams in agricultural watersheds.

Surface water runoff is an important source of water contamination affecting nearby rivers and streams. Many rural creeks are documented habitats for important aquatic species and the focus of restoration activities. In this study, we collected creek water samples in watersheds with a range of commercial-to-agricultural land use during rain events, and applied suspect and non-target screening with high-resolution mass spectrometry (HRMS) to characterize the occurrence of contaminants of emerging concern (CECs). In total, 58 CECs were identified, and 36 of them were confirmed and semi-quantified with reference standards. Pesticides were detected in all land use, including urban/commercial areas. Some pesticides were observed at concentrations of >10,000 ng/L demonstrating the strong contamination input during rain events. Five pesticides (azoxystrobin, fludioxonil, 4-hydroxy-chlorothalonil, imidacloprid, 2-methyl-4-chlorophenoxyacetic acid) were prioritized based on their risk quotients. HRMS chemical profiles demonstrated the wide range of chemical exposures in a given stream system and that compounds associated with specific land uses occur across land uses. Temporal trends suggested that some CECs remain present in creek water for months, resulting in chronic exposures across the life stages of aquatic species. These findings highlight the potential for contamination from agricultural runoff and the associated ecological risk to aquatic species. SYNOPSIS: Suspect and non-target screening revealed the chronic occurrence of emerging contaminants in streams in agricultural catchments during rain events.

Suspect and Nontarget Screening for Contaminants of Emerging Concern in an Urban Estuary.

This study used suspect and nontarget screening with high-resolution mass spectrometry to characterize the occurrence of contaminants of emerging concern (CECs) in the nearshore marine environment of Puget Sound (WA). In total, 87 non-polymeric CECs were identified; those confirmed with reference standards (45) included pharmaceuticals, herbicides, vehicle-related compounds, plasticizers, and flame retardants. Eight polyfluoroalkyl substances were detected; perfluorooctanesulfonic acid (PFOS) concentrations were as high as 72-140 ng/L at one location. Low levels of methamphetamine were detected in 41% of the samples. Transformation products of pesticides were tentatively identified, including two novel transformation products of tebuthiuron. While a hydrodynamic simulation, analytical results, and dilution calculations demonstrated the prevalence of wastewater effluent to nearshore marine environments, the identity and abundance of selected CECs revealed the additional contributions from stormwater and localized urban and industrial sources. For the confirmed CECs, risk quotients were calculated based on concentrations and predicted toxicities, and eight CECs had risk quotients >1. Dilution in the marine estuarine environment lowered the risks of most wastewater-derived CECs, but dilution alone is insufficient to mitigate risks of localized inputs. These findings highlighted the necessity of suspect and nontarget screening and revealed the importance of localized contamination sources in urban marine environments.