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1.
J Clin Microbiol ; 57(11)2019 11.
Article in English | MEDLINE | ID: mdl-31434726

ABSTRACT

Clostridioides difficile infection (CDI) is one of the most common health care-associated infections, resulting in significant morbidity, mortality, and economic burden. Diagnosis of CDI relies on the assessment of clinical presentation and laboratory tests. We evaluated the clinical performance of ultrasensitive single-molecule counting technology for detection of C. difficile toxins A and B. Stool specimens from 298 patients with suspected CDI were tested with the nucleic acid amplification test (NAAT; BD MAX Cdiff assay or Xpert C. difficile assay) and Singulex Clarity C. diff toxins A/B assay. Specimens with discordant results were tested with the cell cytotoxicity neutralization assay (CCNA), and the results were correlated with disease severity and outcome. There were 64 NAAT-positive and 234 NAAT-negative samples. Of the 32 NAAT+/Clarity- and 4 NAAT-/Clarity+ samples, there were 26 CCNA- and 4 CCNA- samples, respectively. CDI relapse was more common in NAAT+/toxin+ patients than in NAAT+/toxin- and NAAT-/toxin- patients. The clinical specificity of Clarity and NAAT was 97.4% and 89.0%, respectively, and overdiagnosis was more than three times more common in NAAT+/toxin- than in NAAT+/toxin+ patients. The Clarity assay was superior to NAATs for the diagnosis of CDI, by reducing overdiagnosis and thereby increasing clinical specificity, and the presence of toxins was associated with negative patient outcomes.


Subject(s)
Clostridium Infections/diagnosis , Enterotoxins/isolation & purification , Immunoassay/methods , Single Molecule Imaging/methods , Adult , Aged , Bacteriological Techniques/methods , Clostridioides difficile/chemistry , Clostridioides difficile/genetics , Clostridium Infections/microbiology , Feces/chemistry , Feces/microbiology , Female , Humans , Male , Medical Overuse , Middle Aged , Nucleic Acid Amplification Techniques , Sensitivity and Specificity
3.
J Clin Microbiol ; 57(3)2019 Mar.
Article in English | MEDLINE | ID: mdl-30814302
4.
J Spec Pediatr Nurs ; 19(2): 127-38, 2014 Apr.
Article in English | MEDLINE | ID: mdl-24502662

ABSTRACT

PURPOSE: This study examined relationships between cumulative trauma (CT) and urban African American (AA) adolescents' blood pressure (BP), heart rate (HR), and perceptions of health. DESIGN AND METHOD: A correlational design using secondary data analysis studied effects of CT, health outcomes, and perceptions of health. Participants were 175 urban AA youth (11-16 years) who completed structured surveys and physiological measures of HR and BP before and after exercise. RESULTS: AA youth were experiencing high levels of CT. Negative correlations were obtained between AA females' perceptions of their health and systolic BP with levels of trauma. No gender differences were found in HR or BP. PRACTICE IMPLICATIONS: AA females with high CT may perceive themselves as less healthy and can be at risk for health problems.


Subject(s)
Adolescent Behavior/ethnology , Adolescent Behavior/physiology , Black or African American/psychology , Cumulative Trauma Disorders/physiopathology , Cumulative Trauma Disorders/psychology , Health Knowledge, Attitudes, Practice/ethnology , Adolescent , Adolescent Behavior/psychology , Age Factors , Blood Pressure/physiology , Child , Female , Heart Rate/physiology , Humans , Male , Risk Factors , Sex Factors , Statistics as Topic , United States , Urban Population
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