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1.
Antimicrob Agents Chemother ; 58(12): 7121-7, 2014 Dec.
Article in English | MEDLINE | ID: mdl-25224009

ABSTRACT

The binding and cytochrome P45051 (CYP51) inhibition properties of a novel antifungal compound, VT-1161, against purified recombinant Candida albicans CYP51 (ERG11) and Homo sapiens CYP51 were compared with those of clotrimazole, fluconazole, itraconazole, and voriconazole. VT-1161 produced a type II binding spectrum with Candida albicans CYP51, characteristic of heme iron coordination. The binding affinity of VT-1161 for Candida albicans CYP51 was high (dissociation constant [Kd], ≤ 39 nM) and similar to that of the pharmaceutical azole antifungals (Kd, ≤ 50 nM). In stark contrast, VT-1161 at concentrations up to 86 µM did not perturb the spectrum of recombinant human CYP51, whereas all the pharmaceutical azoles bound to human CYP51. In reconstitution assays, VT-1161 inhibited Candida albicans CYP51 activity in a tight-binding fashion with a potency similar to that of the pharmaceutical azoles but failed to inhibit the human enzyme at the highest concentration tested (50 µM). In addition, VT-1161 (MIC = 0.002 µg ml(-1)) had a more pronounced fungal sterol disruption profile (increased levels of methylated sterols and decreased levels of ergosterol) than the known CYP51 inhibitor voriconazole (MIC = 0.004 µg ml(-1)). Furthermore, VT-1161 weakly inhibited human CYP2C9, CYP2C19, and CYP3A4, suggesting a low drug-drug interaction potential. In summary, VT-1161 potently inhibited Candida albicans CYP51 and culture growth but did not inhibit human CYP51, demonstrating a >2,000-fold selectivity. This degree of potency and selectivity strongly supports the potential utility of VT-1161 in the treatment of Candida infections.


Subject(s)
14-alpha Demethylase Inhibitors/chemistry , Antifungal Agents/chemistry , Candida albicans/chemistry , Fungal Proteins/antagonists & inhibitors , Pyridines/chemistry , Sterol 14-Demethylase/chemistry , Tetrazoles/chemistry , 14-alpha Demethylase Inhibitors/chemical synthesis , Antifungal Agents/chemical synthesis , Binding Sites , Candida albicans/enzymology , Cytochrome P-450 CYP2C19/chemistry , Cytochrome P-450 CYP2C9/chemistry , Cytochrome P-450 CYP3A/chemistry , Enzyme Assays , Escherichia coli/genetics , Escherichia coli/metabolism , Fungal Proteins/chemistry , Fungal Proteins/genetics , Gene Expression , Humans , Protein Binding , Pyridines/chemical synthesis , Recombinant Proteins/chemistry , Recombinant Proteins/genetics , Species Specificity , Sterol 14-Demethylase/genetics , Tetrazoles/chemical synthesis , Voriconazole/chemistry
2.
Bioresour Technol ; 102(2): 1503-8, 2011 Jan.
Article in English | MEDLINE | ID: mdl-20801645

ABSTRACT

This study demonstrates use of recombinant yeast to simultaneously saccharify and ferment grass juice (GJ) to bioethanol. A modified Bacillus subtilis levanase gene (sacC) in which the native bacterial signal sequence was replaced with a yeast α-factor domain, was synthesised with yeast codon preferences and transformed into Saccharomyces cerevisiae (strain AH22) using the expression vector pMA91. AH22:psacC transformants secreted sacCp as an active, hyper-glycosylated (>180 kDa) protein allowing them to utilise inulin (ß[2-1] linked fructose) and levan (ß[2-6] linkages) as growth substrates. The control (AH22:pMA91) strain, transformed with empty plasmid DNA was not able to utilise inulin or levan. When cultured on untreated GJ levels of growth and bioethanol production were significantly higher in experiments with AH22:psacC than with AH22:pMA91. Bioethanol yields from AH22:psacC grown on GJ (32.7[±4] mg mL(-1)) compared closely to those recently achieved (Martel et al., 2010) using enzymatically pre-hydrolysed GJ (36.8[±4] mg mL(-1)).


Subject(s)
Bacillus subtilis/enzymology , Biofuels/analysis , Ethanol/chemical synthesis , Fermentation , Glycoside Hydrolases/metabolism , Poaceae/metabolism , Saccharomyces cerevisiae/metabolism , Amino Acid Sequence , Bacterial Proteins/chemistry , Biotechnology , Carbohydrate Metabolism , Molecular Sequence Data , Recombination, Genetic/genetics , Saccharomyces cerevisiae/genetics , Saccharomyces cerevisiae/growth & development , Transformation, Genetic
3.
Bioresour Technol ; 101(12): 4395-402, 2010 Jun.
Article in English | MEDLINE | ID: mdl-20153640

ABSTRACT

Microbial inulinases find application in food, pharmaceutical and biofuel industries. Here, a novel Lactobacillus paracasei beta-fructosidase was overexpressed as truncated cytosolic protein ((t)fosEp) in Escherichia coli. Purified (t)fosEp was thermostable (10-50 degrees C) with a pH optimum of 5; it showed highest affinity for bacterial levan (beta[2-6] linked fructose) followed by nystose, chicory inulin, 1-kestose (beta[2-1] linkages) and sucrose (K(m) values of 0.5, 15, 15.6, 49 and 398 mM, respectively). Hydrolysis of polyfructose moieties in agriculturally-sourced grass juice (GJ) with (t)fosEp resulted in the release of >13 mg/ml more bioavailable fructose than was measured in untreated GJ. Bioethanol yields from fermentation experiments with Brewer's yeast and GJ+(t)fosEp were >25% higher than those achieved using untreated GJ feedstock (36.5[+/-4.3] and 28.2[+/-2.7]mg ethanol/ml, respectively). This constitutes the first specific study of the potential to ferment ethanol from grass juice and the utility of a novel core domain of beta-fructosidase from L. paracasei.


Subject(s)
Biofuels , Ethanol/metabolism , Fructans/metabolism , Lactobacillus/enzymology , Poaceae/metabolism , Recombinant Proteins/isolation & purification , beta-Fructofuranosidase/chemistry , Amino Acid Sequence , Electrophoresis, Polyacrylamide Gel , Fermentation , Hydrolysis , Models, Molecular , Molecular Sequence Data , Protein Structure, Tertiary , Recombinant Proteins/chemistry , Recombinant Proteins/metabolism , Solubility , Substrate Specificity , Yeasts/growth & development
4.
Antimicrob Agents Chemother ; 52(10): 3718-24, 2008 Oct.
Article in English | MEDLINE | ID: mdl-18663031

ABSTRACT

The complete DNA sequence of Candida albicans DIT2, encoding cytochrome P450 family 56 (CYP56), was obtained, and heterologous expression was achieved in Escherichia coli, where CYP56 was targeted to the membrane fraction. In reconstituted assays with the purified enzyme, CYP56 was shown to catalyze the conversion of N-formyl tyrosine into N,N'-bisformyl dityrosine, a reaction that was dependent on cytochrome P450 reductase, NADPH, and oxygen, yielding a turnover of 21.6 min(-1) and a k(s) of 26 microM. The Hill number was calculated as 1.6, indicating that two molecules of the substrate could bind to the protein. Azole antifungals could bind to the heme of CYP56 as a sixth ligand with high affinity. Both chromosomal alleles of CYP56 were disrupted using the SAT1 flipper technique, and CYP56 was found to be nonessential for cell viability under the culture conditions investigated. Susceptibility to azole drugs that bind to cytochromes P450 was tested, and the mutant showed unaltered susceptibility. However, the mutant showed increased susceptibility to the echinocandin drug caspofungin, suggesting an alteration in 1,3-glucan synthase and/or cell wall structure mediated by the presence of dityrosine. Phenotypically, the wild-type and mutant strains were morphologically similar when cultured in rich yeast extract-peptone-dextrose medium. However in minimal medium, the cyp56Delta mutant strain exhibited hyphal growth, in contrast to the wild-type strain, which grew solely in the yeast form. Furthermore, CYP56 was essential for chlamydospore formation.


Subject(s)
Candida albicans/drug effects , Candida albicans/enzymology , Cytochrome P-450 Enzyme System/metabolism , Base Sequence , Candida albicans/genetics , Candida albicans/growth & development , Cell Wall/metabolism , Cytochrome P-450 Enzyme System/genetics , DNA Primers/genetics , DNA, Fungal/genetics , Drug Resistance, Fungal/genetics , Drug Resistance, Fungal/physiology , Gene Deletion , Gene Expression , Genes, Fungal , Mutation , Phenotype , Recombinant Proteins/genetics , Recombinant Proteins/metabolism , Tyrosine/analogs & derivatives , Tyrosine/biosynthesis
5.
Biochem Biophys Res Commun ; 286(1): 48-54, 2001 Aug 10.
Article in English | MEDLINE | ID: mdl-11485306

ABSTRACT

Native yeast NADPH-cytochrome P450 oxidoreductase (CPR; EC 1.6.2.4) and a soluble derivative lacking 33 amino acids of the NH(2)-terminus have been overexpressed as recombinant proteins in Escherichia coli. The presence of a hexahistidine sequence at the N-terminus allowed protein purification in a single step using nickel-chelating affinity chromatography. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis confirmed the predicted molecular weights of the proteins and indicated a purity of >95%. Protein functionality was demonstrated by cytochrome c reduction and reconstitution of CYP61-mediated sterol Delta(22)-desaturation. Steady-state kinetics of cytochrome c reductase activity revealed a random Bi-Bi mechanism with NADPH donating electrons directly to CPR to produce a reduced intermediary form of the enzyme. The kinetic mechanism studies showed no difference between the two yeast CPRs in mechanism or after reconstitution with CYP61-mediated 22-desaturation, confirming that the retention of the NH(2)-terminable membrane anchor is functionally dispensable.


Subject(s)
NADPH-Ferrihemoprotein Reductase/metabolism , Cytochrome P-450 Enzyme System/metabolism , Electrophoresis, Polyacrylamide Gel , Kinetics , NADP/metabolism , NADPH-Ferrihemoprotein Reductase/isolation & purification , Oxidoreductases/metabolism , Saccharomyces cerevisiae/enzymology , Saccharomyces cerevisiae Proteins
6.
Biochem Biophys Res Commun ; 284(3): 845-9, 2001 Jun 15.
Article in English | MEDLINE | ID: mdl-11396979

ABSTRACT

Azole fungicides were thought to have much greater affinity for the fungal cytochrome P450 enzyme, sterol 14 alpha-demthylase (CYP51) than the plant orthologue. Using purified CYP51 from the plant Sorghum bicolor L Moenech, a direct comparison of the sensitivity to the fungicides triadimenol and tebuconazole has been carried out. S. bicolor CYP51 was purified to homogenity as determined by SDS--PAGE and specific heme content. Addition of the azole fungicides triadimenol and tebuconazole induced type II spectral changes, with saturation occurring at equimolar azole/P450 concentrations. Inhibition of reconstituted activities revealed only a threefold insensitivity of the plant CYP51 compared to a fungal CYP51, from the phytopathogen Ustilago maydis, as judged by IC(50) values. The implications for fungicide mode of action and application are discussed.


Subject(s)
Cytochrome P-450 Enzyme Inhibitors , Fungicides, Industrial/pharmacology , Oxidoreductases/antagonists & inhibitors , Plant Proteins/antagonists & inhibitors , Triazoles/pharmacology , Cytochrome P-450 Enzyme System/metabolism , Escherichia coli/genetics , Fungal Proteins/antagonists & inhibitors , Fungal Proteins/metabolism , Fungicides, Industrial/metabolism , Inhibitory Concentration 50 , Oxidoreductases/metabolism , Plant Proteins/metabolism , Spectrophotometry , Sterol 14-Demethylase , Triazoles/metabolism
7.
J Exp Bot ; 51(347): 985-93, 2000 Jun.
Article in English | MEDLINE | ID: mdl-10948226

ABSTRACT

A cyanoalanine synthase and two isoforms (A, cytosolic and B, chloroplastic) of cysteine synthase (O:-acetylserine (thiol) lyase) were isolated from spinach. N-terminal amino acid sequence analysis of the cyanoalanine synthase gave 100% homology for the determined 12 residues with a published sequence for the mitochondrial cysteine synthase isoform. All three enzymes catalysed both the cysteine synthesis and cyanoalanine synthesis reactions, although with different efficiencies. Michaelis-Menten kinetics were observed for all three enzymes when substrate saturation experiments were performed varying O:-acetylserine, chloroalanine and cysteine. Negative co-operative kinetics were observed for cysteine synthases A and B when substrate saturation experiments were performed varying sulphide and cyanide, compared with the Michaelis-Menten kinetics observed for cyanoalanine synthase. The exception was negative co-operativity observed towards sulphide for cyanoalanine synthase with O:-acetylserine as co-substrate. The optimum sulphide concentration was dependent on the alanyl co-substrate used. The amino acid sequence similarity places these three enzymes in the same gene family, and whilst the close kinetic similarities support this, they also indicate distinct roles for the isoforms.


Subject(s)
Chloroplasts/enzymology , Cysteine Synthase/metabolism , Lyases/metabolism , Mitochondria/enzymology , Spinacia oleracea/enzymology , Amino Acid Sequence , Cysteine Synthase/chemistry , Cysteine Synthase/isolation & purification , Isoenzymes/chemistry , Isoenzymes/isolation & purification , Isoenzymes/metabolism , Kinetics , Lyases/chemistry , Lyases/isolation & purification , Molecular Sequence Data , Substrate Specificity
8.
Plant J ; 12(4): 875-84, 1997 Oct.
Article in English | MEDLINE | ID: mdl-9375399

ABSTRACT

A cDNA encoding a high-affinity sulphate transporter has been isolated from barley by complementation of a yeast mutant. The cDNA, designated HVST1, encodes a polypeptide of 660 amino acids (M(r) = 72,550), which is predicted to have 12 membrane-spanning domains and has extensive sequence homology with other identified eukaryotic sulphate transporters. The K(m) for sulphate was 6.9 microM when the HVST1 cDNA was expressed in a yeast mutant deficient in the gene encoding for the yeast SUL1 sulphate transporter. The strong pH-dependency of sulphate uptake when HVST1 was expressed heterologously in yeast suggests that the HVST1 polypeptide is a proton/sulphate co-transporter. The gene encoding HVST1 is expressed specifically in root tissues and the abundance of the mRNA is strongly influenced by sulphur nutrition. During sulphur-starvation of barley, the abundance of mRNA corresponding to HVST1, and the capacity of the roots to take up sulphate, both increase. Upon re-supply of sulphate, the abundance of the mRNA corresponding to HVST1, and the capacity of the roots to take up sulphate, decrease rapidly, concomitant with rises in tissue sulphate, cysteine and glutathione contents. Addition of the cysteine precursor, O-acetylserine, to plants grown with adequate sulphur supply, leads to increases in sulphate transporter mRNA, sulphate uptake rates and tissue contents of glutathione and cysteine. It is suggested, that whilst sulphate, cysteine and glutathione may be candidates for negative metabolic regulators of sulphate transporter gene expression, this regulation may be overridden by O-acetylserine acting as a positive regulator.


Subject(s)
Carrier Proteins/genetics , Gene Expression Regulation, Plant , Hordeum/genetics , Membrane Transport Proteins , Plant Roots/genetics , Sulfates/metabolism , Amino Acid Sequence , Biological Transport , Carrier Proteins/chemistry , DNA, Complementary/biosynthesis , Molecular Sequence Data , Molecular Weight , Sulfate Transporters
9.
Phytochemistry ; 35(4): 863-8, 1994 Mar.
Article in English | MEDLINE | ID: mdl-7764629

ABSTRACT

The pectinesterases in tomato pericarp were fractionated by cation exchange into four forms (A-D). Form A was the most abundant and C the second most abundant. Forms A-C were further purified by gel filtration, and antibodies were raised against A and C. Comparison of the different forms by dot blots and Western blots showed that although all three forms shared common immunological characteristics, there were also differences in their behaviour, indicative of structural differences. Form A had an N-terminal sequence identical to that published previously for the major pectinesterase in tomato fruit. In contrast, form C had a completely novel N-terminal sequence. Form A was absent from hypocotyls and roots. Forms B and C were present in comparable amounts in hypocotyls, while from C predominated in roots.


Subject(s)
Carboxylic Ester Hydrolases/chemistry , Vegetables/enzymology , Amino Acid Sequence , Carboxylic Ester Hydrolases/immunology , Molecular Sequence Data
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