ABSTRACT
A total of 570 lyophilised Brassica root and shoot tissue samples were hydrolyzed, and the liberated isothiocyanates (ITCs) were analyzed by gas chromatography-flame photometric detection (GC-FPD). Glucosinolates (GSLs) were extracted from samples of the same tissues and analyzed by high-performance liquid chromatography (HPLC). The concentrations of six GSLs/ITCs (2-propenyl, 3-butyl, 4-pentenyl, benzyl, 4-methylthiobutyl, and 2-phenylethyl) as determined by the two techniques were compared. In 79% of the samples, the concentration of GSLs in the tissues was greater than that of the ITCs released on hydrolysis. Several possible reasons for the difference are proposed, including the effect of tissue storage time, hydrolysis of GSLs may be less efficient than the GSL extraction procedure, or some of the ITCs formed reacted with plant proteins and amino acids in the sample and were therefore not detected in the extract. GSL concentration in plant tissues is used to estimate the biofumigation potential of the plant tissue, whereas the actual biofumigation effect is thought to be due to the ITCs formed by hydrolysis of the plant-based GSLs. The variation between ITC and GSL values therefore has implications for the assessment of the biofumigation potential of the plant tissue.
Subject(s)
Brassica/metabolism , Glucosinolates/metabolism , Isothiocyanates/metabolism , Chromatography, Gas , Chromatography, High Pressure Liquid , Hydrolysis , Pest Control, BiologicalABSTRACT
Fifty-five patients were randomized in a double-blinded manner to large loop excision of the transformation zone (LLETZ) (29 patients) or radical cervical diathermy with fulgaration (RCDF) (26 patients) for treatment of cervical intraepithelial neoplasia (CIN). Patients completed a 2-month diary of symptoms and completed a 10-week postoperative questionnaire. White vaginal discharge lasted 7.3 (4.6) days (SD) for RCDF compared to 3.4 (1.6) days (SD) for LLETZ. There was no significant difference in any other symptoms on analysis This appears to be the first report clearly assessing the length and severity of postoperative symptoms following LLETZ or RCDF. There is no reason on length and severity of postoperative symptoms to choose 1 of these 2 treatment methods over the other.
Subject(s)
Electrocoagulation , Uterine Cervical Dysplasia/surgery , Uterine Cervical Neoplasms/surgery , Adult , Double-Blind Method , Female , Humans , Postoperative Period , Prospective Studies , Treatment OutcomeSubject(s)
Genital Neoplasms, Female/therapy , Carcinoma/therapy , Carcinoma in Situ/diagnosis , Carcinoma in Situ/therapy , Female , Humans , Ovarian Neoplasms/diagnosis , Ovarian Neoplasms/drug therapy , Ovarian Neoplasms/radiotherapy , Ovarian Neoplasms/surgery , Uterine Cervical Dysplasia/diagnosis , Uterine Cervical Neoplasms/diagnosis , Uterine Cervical Neoplasms/therapy , Uterine Neoplasms/diagnosis , Uterine Neoplasms/therapyABSTRACT
Venereal diseases, or sexually transmitted diseases as they are more aptly named, are emerging as a major medical problem. Although syphilis and gonorrhoea are the traditional venereal diseases, it is now widely accepted that sexual transmission occurs, at least to some degree, in a wide range of relatively common infective disorders. For the family physician coping with this group of diseases, there are a number of problems which include diagnostic difficulties, adequate supervision of treatment and general care and confidentiality within the family situation.
