ABSTRACT
A nine year aged male presented with facial lesions and the problem of responding to conventional treatment of leishmaniasis. Multiple injections of antimony and several topical ointments had been administered in hospital but fresh lesions erupted with potential to disfigure. Smears examined from nodular lesions confirmed presence of Leishmania amastigotes and parenteral pentostam was commenced for over eight weeks. A partial clinical outcome was achieved judged by extent of re-epithelialisation. Combined therapy of pentostam and oral allopurinol at a dose of 7mg/kg/day was started and finalised at 120 days. All facial lesions receded and 100% re-epithelialisation of the lesions established.
Subject(s)
Allopurinol/therapeutic use , Antimony Sodium Gluconate/therapeutic use , Antiprotozoal Agents/therapeutic use , Leishmaniasis, Cutaneous/drug therapy , Administration, Oral , Child , Drug Therapy, Combination , Humans , Kenya , MaleABSTRACT
Three diagnostic tests for visceral leishmaniasis (VL), the freeze-dried direct agglutination test (FD-DAT), the rK39 dipstick and a urine latex antigen test (KAtex), were evaluated for use in primary care in East Africa and the Indian subcontinent. Clinical suspects were prospectively recruited and tissue, blood and urine samples were taken. Direct microscopic examination of tissue smear, and FD-DAT, rK39 and KAtex were performed. Sensitivity and specificity with 95% credible intervals were estimated using Bayesian latent class analysis. On the Indian subcontinent both the FD-DAT and the rK39 strip test exceeded the 95% sensitivity and 90% specificity target, but not so in East Africa. Sensitivity of the FD-DAT was high in Ethiopia and Kenya but lower in Sudan, while its specificity was below 90% in Kenya. Sensitivity of the rK39 was below 80% in the three countries, and its specificity was only 70% in Ethiopia. KAtex showed moderate to very low sensitivity in all countries. FD-DAT and rK39 can be recommended for clinical practice on the Indian subcontinent. In East Africa, their clinical use should be carefully monitored. More work is needed to improve existing formats, and to develop better VL diagnostics.
Subject(s)
Agglutination Tests/standards , Leishmaniasis, Visceral/diagnosis , Reagent Kits, Diagnostic/standards , Adolescent , Adult , Africa, Eastern , Asia, Western , Case-Control Studies , Child , Child, Preschool , Female , Humans , Infant , Leishmaniasis, Visceral/parasitology , Male , Middle Aged , Prospective Studies , Reagent Strips/standards , Reproducibility of Results , Sensitivity and SpecificityABSTRACT
Human immunodeficiency virus type 1 (HIV-1) epidemiology among residents of a rural agricultural plantation in Kericho, Kenya was studied. HIV-1 prevalence was 14.3%, and was higher among women (19.1%) than men (11.3%). Risk factors associated with HIV-1 for men were age (>or=25 years), marital history (one or more marriages), age difference from current spouse (>or=5 years), Luo ethnicity, sexually transmitted infection (STI) symptoms in the past 6 months, circumcision (protective), and sexual activity (>or=7 years). Among women, risk factors associated with HIV-1 were age (25-29 years, >or=35 years), marital history (one or more marriages), age difference from current spouse (>or=10 years), Luo ethnicity, STI symptoms in the past 6 months, and a STI history in the past 5 years. Most participants (96%) expressed a willingness to participate in a future HIV vaccine study. These findings will facilitate targeted intervention and prevention measures for HIV-1 infection in Kericho.
Subject(s)
HIV Infections/epidemiology , HIV-1/isolation & purification , Adolescent , Adult , Age Factors , Ethnicity , Female , HIV Infections/virology , Humans , Kenya/epidemiology , Male , Middle Aged , Prevalence , Risk Factors , Rural Population , Sex FactorsABSTRACT
We report a rare case of visceral leishmanisis with concomitant post kala-azar dermal leishmaniasis as the initial presentation in a female patient from Baringo district, Rift valley province, Kenya.
Subject(s)
Aminoquinolines/therapeutic use , Antiprotozoal Agents/therapeutic use , Leishmaniasis, Cutaneous/drug therapy , Leishmaniasis, Visceral/drug therapy , Administration, Oral , Adolescent , Animals , Female , Humans , Leishmaniasis, Cutaneous/diagnosis , Leishmaniasis, Cutaneous/etiology , Leishmaniasis, Visceral/complications , Leishmaniasis, Visceral/diagnosisSubject(s)
Culture Media, Serum-Free/standards , Leishmania donovani/growth & development , Leishmaniasis, Visceral/diagnosis , Leishmaniasis, Visceral/parasitology , Spleen/parasitology , Animals , Culture Media, Serum-Free/economics , Humans , Kenya , Reproducibility of Results , Suction , Time FactorsABSTRACT
Cultivated Plasmodium falciparum gametocytes reach maturity in vitro in approximately 14-16 days, during which they pass through five morphologically distinct developmental stages. Purification of the earlier developmental stages has not been previously reported. We have modified the standard discontinuous Percoll gradient method for the separation of stage IV and V gametocytes to obtain enriched preparations of those and the earlier P. falciparum gametocyte stages. In contrast to the stages II, III, and IV, the mature stage V gametocytes from our gradient readily transformed into gametes. Such preparations may be useful in research studies on the mechanisms that underlie gametocytogenesis.
