ABSTRACT
Fetal hemoglobin was measured in HIV 1/2 patients under treatment with combined therapy (zidovudine and a protease inhbitor). A total of 143 patientes and 103 normal individual were investigated by the quantitative method of Betke and the semi-quantitative acid elution method of Keihauer. In the normal person, hemoglobin F makes up less than.
Subject(s)
HIV-1 , HIV , HIV-2 , Fetal Hemoglobin , Hemoglobins , ZidovudineABSTRACT
Fetal hemoglobin was measured in HIV1/2 patients under treatment with combined therapy (zidovudine and a protease inhibitor). A total of 143 patients and 103 normal individuals were investigated by the quantitative method of Betke and the semi-quantitative acid elution method of Kleihauer. In the normal person, hemoglobin F makes up less than 1 percent and an increase higher than 1.5 percent was observed in 21.4 percent of HIV patients by the method of Betke and in 24.8 percent of HIV-infected patients by the method of Kleihauer. The quantitative biochemical method of Betke showed that the populations were significantly different (two-tailed Mann-Whitney test). The reason for this hemoglobin F increase might be ascribed to the effect of zidovudine or to direct viral action on gamma chain expression. The finding of a higher F cell frequency indicated by the method of Kleihauer rather suggests that there is an increased F cell clone proliferation rather than an increase in hemoglobin F level in every cell
Subject(s)
Humans , Fetal Hemoglobin/analysis , HIV Infections/blood , Anti-HIV Agents/therapeutic use , Case-Control Studies , Chi-Square Distribution , Erythroid Precursor Cells/drug effects , HIV Infections/drug therapy , HIV Protease Inhibitors/therapeutic use , Statistics, Nonparametric , Zidovudine/therapeutic useABSTRACT
Fetal hemoglobin was measured in HIV1/2 patients under treatment with combined therapy (zidovudine and a protease inhibitor). A total of 143 patients and 103 normal individuals were investigated by the quantitative method of Betke and the semi-quantitative acid elution method of Kleihauer. In the normal person, hemoglobin F makes up less than 1% and an increase higher than 1.5% was observed in 21.4% of HIV patients by the method of Betke and in 24.8% of HIV-infected patients by the method of Kleihauer. The quantitative biochemical method of Betke showed that the populations were significantly different (two-tailed Mann-Whitney test). The reason for this hemoglobin F increase might be ascribed to the effect of zidovudine or to direct viral action on gamma chain expression. The finding of a higher F cell frequency indicated by the method of Kleihauer rather suggests that there is an increased F cell clone proliferation rather than an increase in hemoglobin F level in every cell.
