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Eur J Immunol ; 30(11): 3100-10, 2000 Nov.
Article in English | MEDLINE | ID: mdl-11093124

ABSTRACT

We have identified a novel Kruppel-type zinc finger (ZF) gene, SKAT-2, which is selectively expressed by murine Th2 cells. The protein encoded by this gene has 14 C2H2-type ZF tandemly arrayed at its C terminus and N-terminal SCAN box and KRAB domains. SKAT-2 is tissue restricted in expression at the RNA level, detectable only in brain and at low levels in kidney and spleen and few hematopoietic cell lines. By in situ hybridization, SKAT-2 expression was found to peak in antigen-stimulated CD4(+) T cells after 2-3 days of culture under Th2 but not Th1 biasing conditions. This pattern of expression closely mirrored that of GATA-3 in the same cells. In transient transfection experiments in phorbol 12-myristate 13-acetate/ionomycin-stimulated EL4 cells, SKAT-2 was found to up-regulate the activity of the IL-4 but not the IL-5 promoter, contrasting with the ability of GATA-3 to activate both promoters. This result was confirmed using clones of EL4 cells stably expressing an inducible form of SKAT-2, thus SKAT-2 is a novel Th2-specific gene that may play a role in selective regulation of cytokine genes in T cells.


Subject(s)
DNA-Binding Proteins/genetics , Nerve Tissue Proteins , Proteins/genetics , Proteins/immunology , Th2 Cells/immunology , Amino Acid Sequence , Base Sequence , DNA-Binding Proteins/immunology , GATA3 Transcription Factor , Gene Expression/immunology , Humans , Molecular Sequence Data , Trans-Activators/genetics , Trans-Activators/immunology , Zinc Fingers
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