Your browser doesn't support javascript.
loading
Show: 20 | 50 | 100
Results 1 - 3 de 3
Filter
1.
J Biomed Opt ; 23(9): 1-4, 2018 09.
Article in English | MEDLINE | ID: mdl-30251491

ABSTRACT

Treatment outcomes for brain cancer have seen dismal improvements over the last two decades as evident in available statistical data. Efforts to address this challenge include development of near-infrared contrast agents for improvements in diagnostic and therapeutic modalities. This creates a need for imaging technologies that can support the intraoperative use of such agents. Here, we report implementation of a recently introduced augmented microscope in combination with indocyanine green (ICG), a near-infrared contrast agent, for surgical image guidance of a glioma resection in a rat model. Luc-C6 cells were implanted in rats in the left-frontal lobe and grown for 22 days. Surgical resection was performed by a neurosurgeon using the augmented microscope with ICG contrast. ICG accumulated in the tumor tissue due to enhanced permeation and retention from the compromised blood-brain barrier. Videos and images were acquired to evaluate image quality and resection margins. The augmented microscope highlighted tumor tissue regions via visualization of ICG fluorescence and was capable of guiding the rat glioma resection.


Subject(s)
Brain Neoplasms , Image Interpretation, Computer-Assisted/methods , Indocyanine Green/chemistry , Intraoperative Care/methods , Microscopy, Fluorescence/methods , Animals , Brain/diagnostic imaging , Brain/pathology , Brain/surgery , Brain Neoplasms/diagnostic imaging , Brain Neoplasms/pathology , Brain Neoplasms/surgery , Cell Line, Tumor , Female , Neoplasms, Experimental/diagnostic imaging , Neoplasms, Experimental/pathology , Neoplasms, Experimental/surgery , Rats , Rats, Wistar
2.
J Biomed Opt ; 20(10): 106002, 2015 Oct.
Article in English | MEDLINE | ID: mdl-26440760

ABSTRACT

Intraoperative applications of near-infrared (NIR) fluorescent contrast agents can be aided by instrumentation capable of merging the view of surgical field with that of NIR fluorescence. We demonstrate augmented microscopy, an intraoperative imaging technique in which bright-field (real) and electronically processed NIR fluorescence (synthetic) images are merged within the optical path of a stereomicroscope. Under luminance of 100,000 lx, representing typical illumination of the surgical field, the augmented microscope detects 189 nM concentration of indocyanine green and produces a composite of the real and synthetic images within the eyepiece of the microscope at 20 fps. Augmentation described here can be implemented as an add-on module to visualize NIR contrast agents, laser beams, or various types of electronic data within the surgical microscopes commonly used in neurosurgical, cerebrovascular, otolaryngological, and ophthalmic procedures.


Subject(s)
Fluorescent Dyes , Image Enhancement/instrumentation , Microscopy, Fluorescence/instrumentation , Microsurgery/instrumentation , Subtraction Technique/instrumentation , Surgery, Computer-Assisted/instrumentation , Computer Systems , Equipment Design , Equipment Failure Analysis , Infrared Rays , Reproducibility of Results , Sensitivity and Specificity
3.
Neurosurgery ; 11 Suppl 2: 252-7; discussion 257-8, 2015 Jun.
Article in English | MEDLINE | ID: mdl-25710107

ABSTRACT

BACKGROUND: Preservation of adequate blood flow and exclusion of flow from lesions are key concepts of vascular neurosurgery. Indocyanine green (ICG) fluorescence videoangiography is now widely used for the intraoperative assessment of vessel patency. OBJECTIVE: Here, we present a proof-of-concept investigation of fluorescence angiography with augmented microscopy enhancement: real-time overlay of fluorescence videoangiography within the white light field of view of conventional operative microscopy. METHODS: The femoral artery was exposed in 7 anesthetized rats. The dissection microscope was augmented to integrate real-time electronically processed near-infrared filtered images with conventional white light images seen through the standard oculars. This was accomplished by using an integrated organic light-emitting diode display to yield superimposition of white light and processed near-infrared images. ICG solution was injected into the jugular vein, and fluorescent femoral artery flow was observed. RESULTS: Fluorescence angiography with augmented microscopy enhancement was able to detect ICG fluorescence in a small artery of interest. Fluorescence appeared as a bright-green signal in the ocular overlaid with the anatomic image and limited to the anatomic borders of the femoral artery and its branches. Surrounding anatomic structures were clearly visualized. Observation of ICG within the vessel lumens permitted visualization of the blood flow. Recorded video loops could be reviewed in an offline mode for more detailed assessment of the vasculature. CONCLUSION: The overlay of fluorescence videoangiography within the field of view of the white light operative microscope allows real-time assessment of the blood flow within vessels during simultaneous surgical manipulation. This technique could improve intraoperative decision making during complex neurovascular procedures.


Subject(s)
Angiography, Digital Subtraction/methods , Fluorescein Angiography/methods , Indocyanine Green , Neurosurgical Procedures/methods , Vascular Surgical Procedures/methods , Animals , Blood Circulation/physiology , Coloring Agents , Male , Rats , Video Recording/methods
SELECTION OF CITATIONS
SEARCH DETAIL
...