ABSTRACT
The activity of the pH 7.5 NADH-linked nitrate reductase isoform from soybean seedlings is termed inducible. Activity is present only in the leaves of seedlings which have been supplied nitrate. A cDNA clone that encoded part of the mRNA for squash nitrate reductase hybridized specifically with mRNA for this inducible nitrate reductase isoform. Nitrate induction resulted in an increase in the steady-state levels of mRNA for this isoform after 24 hours, while the addition of glutamine to the nitrate diminished steady-steady state levels of this mRNA.
Subject(s)
Isoenzymes/genetics , Nitrate Reductases/genetics , Plants/enzymology , Transcription, Genetic , Enzyme Induction , Isoenzymes/biosynthesis , Nitrate Reductase , Nitrate Reductases/biosynthesis , Nitrate Reductases/metabolism , Plants/genetics , Glycine maxABSTRACT
Tumor formation in cucurbit cultivars resulting from infection by various strains of Agrobacterium tumefaciens and Agrobacterium rhizogenes is environmentally affected. In all instances, tumors could be induced on excised cotyledons while inoculating attached cotyledons or stems resulted in no tumor formation. In addition, buttercup squash (Cucurbita maxima Duch. buttercup) was most susceptible to tumor formation, while butterbush squash (Cucurbita maxima Duch. butterbush) failed to form tumors when inoculated with any of the strains of Agrobacterium. Other tested cucurbit cultivars showed intermediate susceptibility to tumor induction by the various Agrobacterium strains.
