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1.
Biol Neonate ; 81(1): 23-8, 2002 Jan.
Article in English | MEDLINE | ID: mdl-11803173

ABSTRACT

The leucine turnover in newborn infants is influenced by factors such as nutritional state and corticosteroid treatment. Little is known about maternal factors influencing the leucine turnover in the newborn. In order to approach the effect of preeclampsia in the mother on neonatal protein turnover, we studied the leucine turnover in preterm infants soon after birth and again after 7 days. Ten infants from preeclamptic mothers (birth weight 1,280 +/- 240 g, gestational age 31 +/- 2 weeks) and 15 control patients (birth weight 1,320 +/- 210 g, gestational age 30 +/- 2 weeks) were enrolled. The leucine turnover was measured using a primed constant 5-hour intravenous infusion of [1-(13)C]leucine within the first 24 h after delivery and again on day 7 of life. The turnover (leucine flux; micromol.kg(-1).h(-1)) was calculated from the enrichment in alpha-ketoisocaproic acid in plasma. The leucine turnover on day 1 was 300 +/- 65 in the preeclampsia group and 358 +/- 70 in the controls (ANOVA, p < 0.05). The values on day 7 were 474 +/- 73 in the preeclampsia group and 485 +/- 80 in the control group (n.s.). To conclude, the leucine turnover on day 1 is lower in infants of preeclamptic mothers as compared with controls. This difference has disappeared on day 7 of life after receiving the same protein and energy intake.


Subject(s)
Infant, Premature/blood , Leucine/blood , Pre-Eclampsia/blood , Aging , Birth Weight , Carbon Isotopes , Dietary Proteins/administration & dosage , Energy Intake , Female , Gestational Age , Humans , Infant, Newborn , Keto Acids/blood , Pregnancy , Proteins/metabolism
2.
Pediatr Res ; 49(3): 373-8, 2001 Mar.
Article in English | MEDLINE | ID: mdl-11228263

ABSTRACT

The objective of this study was to determine whether insulin administration would prevent the well-documented catabolic effect of dexamethasone given to preterm infants with chronic lung disease. We studied leucine metabolism in 11 very-low-birth-weight infants before dexamethasone treatment and on d 2, 4, and 7 thereafter. During the first 4 d of dexamethasone, insulin was administered i.v. at a dose of 0.5 (n = 7) or 1.0 (n = 5) IU/kg/d. Leucine turnover was not significantly different between d 0 (337 +/- 41.3 micromol leucine/kg/h), d 2 (288 +/- 27.2 micromol leucine/kg/h), d 4 (302 +/- 22.1 micromol leucine/kg/h), and d 7 (321 +/- 21.2 micromol leucine/kg/h), and neither was leucine breakdown (272 +/- 21.9 micromol leucine/kg/h on d 0, 225 +/- 21.5 micromol leucine/kg/h on d 2, 231 +/- 21 micromol leucine/kg/h on d 4, and 242 +/- 17.6 micromol leucine/kg/h on d 7). Weight gain rates were significantly lower during the first week of dexamethasone treatment compared with the week before treatment or the second and third week. We conclude that during insulin and corticosteroid administration in very-low-birth-weight infants, no changes were observed in leucine kinetics in contrast to previous studies. The decrease in weight gain was not reversed.


Subject(s)
Anti-Inflammatory Agents/administration & dosage , Dexamethasone/administration & dosage , Hypoglycemic Agents/administration & dosage , Infant, Premature , Insulin/administration & dosage , Leucine/metabolism , Lung Diseases/drug therapy , Lung Diseases/metabolism , Drug Interactions , Humans , Infant, Newborn , Infant, Very Low Birth Weight , Lung Diseases/physiopathology
3.
Pediatr Res ; 40(1): 169-74, 1996 Jul.
Article in English | MEDLINE | ID: mdl-8798265

ABSTRACT

Infants fed formulas devoid of long-chain polyunsaturated fatty acids (LCP) exhibit low plasma LCP concentrations and have poorer retinal and neurologic development in comparison with their human milk-fed counterparts. It is not known whether the low plasma LCP concentrations result from an impaired biosynthetic capacity, a high need or a low dietary intake. With stable isotope technology and high sensitivity tracer detection using gas chromatography-isotope ratio mass spectrometry we measured the conversion of [13C]linoleic acid (C18:2n-6) and [13C]linolenic acid (C18:3n-3) into their longer chain derivatives in five 1-mo-old formula-fed preterm infants (birth weight 1.17 +/- 0.12.kg and gestational age 28.4 +/- 1.3 wk). Carbon-13-labeled linoleic acid and inolenic were mixed with the formula and administered continuously for 48 h. Both tracers were rapidly incorporated in plasma phospholipids, and their metabolic products including arachidonic acid (C20:4n-6) and docosahexaenoic acid (C22:6n-3) became highly enriched. We demonstrate that the preterm infant is capable of synthesizing LCP from their 18-carbon precursors, and our data do not support the hypothesis that a reduced delta 6 desaturation is a main factor leading to low arachidonic acid and docosahexaenoic acid levels.


Subject(s)
Arachidonic Acid/biosynthesis , Docosahexaenoic Acids/metabolism , Infant, Premature/metabolism , Infant, Very Low Birth Weight/metabolism , Linoleic Acids/metabolism , alpha-Linolenic Acid/metabolism , Carbon Isotopes , Gas Chromatography-Mass Spectrometry/methods , Humans , Infant, Newborn , Linoleic Acid , Phospholipids/blood
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