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1.
J Chem Inf Model ; 61(11): 5589-5600, 2021 11 22.
Article in English | MEDLINE | ID: mdl-34633194

ABSTRACT

Here, we report the implementation and application of a simple, structure-aware framework to generate target-specific screening libraries. Our approach combines advances in generative artificial intelligence (AI) with conventional molecular docking to explore chemical space conditioned on the unique physicochemical properties of the active site of a biomolecular target. As a demonstration, we used our framework, which we refer to as sample-and-dock, to construct focused libraries for cyclin-dependent kinase type-2 (CDK2) and the active site of the main protease (Mpro) of the SARS-CoV-2 virus. We envision that the sample-and-dock framework could be used to generate theoretical maps of the chemical space specific to a given target and so provide information about its molecular recognition characteristics.


Subject(s)
Artificial Intelligence , COVID-19 , Antiviral Agents , Humans , Molecular Docking Simulation , Protease Inhibitors , SARS-CoV-2
2.
Org Biomol Chem ; 18(45): 9181-9190, 2020 12 07.
Article in English | MEDLINE | ID: mdl-33155598

ABSTRACT

This review describes O2-dependent photoreactions for possible routes to double-bond isomerizations. E,Z-isomerizations triggered by O2 and visible light are a new area of potential synthetic interest. The reaction involves the reversible addition of O2 to form a peroxy intermediate with oxygen evolution and partial regeneration of the compound as its isomer. Targeting of O2-dependent photoisomerizations also relates to a practical use of visible light, for example the improved light penetration depth for visible as opposed to UV photons in batch sensitized reactions. This review is intended to draw a link between visible-light formation of a peroxy intermediate and its dark degradation with O2 release for unsaturated compound isomerization. This review should be of interest both to photochemists and synthetic organic chemists, as it ties together mechanistic and synthetic work, drawing attention to an overlooked subject.

3.
Proc Natl Acad Sci U S A ; 115(37): 9228-9233, 2018 09 11.
Article in English | MEDLINE | ID: mdl-30150385

ABSTRACT

Histone posttranslational modifications (PTMs) regulate chromatin dynamics, DNA accessibility, and transcription to expand the genetic code. Many of these PTMs are produced through cellular metabolism to offer both feedback and feedforward regulation. Herein we describe the existence of Lys and Arg modifications on histones by a glycolytic by-product, methylglyoxal (MGO). Our data demonstrate that adduction of histones by MGO is an abundant modification, present at the same order of magnitude as Arg methylation. These modifications were detected on all four core histones at critical residues involved in both nucleosome stability and reader domain binding. In addition, MGO treatment of cells lacking the major detoxifying enzyme, glyoxalase 1, results in marked disruption of H2B acetylation and ubiquitylation without affecting H2A, H3, and H4 modifications. Using RNA sequencing, we show that MGO is capable of altering gene transcription, most notably in cells lacking GLO1. Finally, we show that the deglycase DJ-1 protects histones from adduction by MGO. Collectively, our findings demonstrate the existence of a previously undetected histone modification derived from glycolysis, which may have far-reaching implications for the control of gene expression and protein transcription linked to metabolism.


Subject(s)
Arginine/metabolism , Histones/metabolism , Lactoylglutathione Lyase/metabolism , Protein Processing, Post-Translational/drug effects , Pyruvaldehyde , Transcription, Genetic/drug effects , HEK293 Cells , Humans , Pyruvaldehyde/metabolism , Pyruvaldehyde/pharmacology
4.
Nucleic Acids Res ; 46(7): 3458-3467, 2018 04 20.
Article in English | MEDLINE | ID: mdl-29438559

ABSTRACT

Reactive oxygen species (ROS) are formed in mitochondria during electron transport and energy generation. Elevated levels of ROS lead to increased amounts of mitochondrial DNA (mtDNA) damage. We report that levels of M1dG, a major endogenous peroxidation-derived DNA adduct, are 50-100-fold higher in mtDNA than in nuclear DNA in several different human cell lines. Treatment of cells with agents that either increase or decrease mitochondrial superoxide levels leads to increased or decreased levels of M1dG in mtDNA, respectively. Sequence analysis of adducted mtDNA suggests that M1dG residues are randomly distributed throughout the mitochondrial genome. Basal levels of M1dG in mtDNA from pulmonary microvascular endothelial cells (PMVECs) from transgenic bone morphogenetic protein receptor 2 mutant mice (BMPR2R899X) (four adducts per 106 dG) are twice as high as adduct levels in wild-type cells. A similar increase was observed in mtDNA from heterozygous null (BMPR2+/-) compared to wild-type PMVECs. Pulmonary arterial hypertension is observed in the presence of BMPR2 signaling disruptions, which are also associated with mitochondrial dysfunction and oxidant injury to endothelial tissue. Persistence of M1dG adducts in mtDNA could have implications for mutagenesis and mitochondrial gene expression, thereby contributing to the role of mitochondrial dysfunction in diseases.


Subject(s)
DNA, Mitochondrial/metabolism , Mitochondria/genetics , Oxidative Stress/genetics , Purine Nucleosides/metabolism , Animals , Bone Morphogenetic Protein Receptors, Type II/genetics , DNA Adducts/genetics , DNA Adducts/metabolism , DNA, Mitochondrial/genetics , Electron Transport/drug effects , Endothelial Cells/drug effects , Endothelial Cells/metabolism , Gene Expression Regulation/genetics , Humans , Hypertension, Pulmonary/genetics , Hypertension, Pulmonary/metabolism , Hypertension, Pulmonary/pathology , Lipid Peroxidation/genetics , Mice , Mice, Transgenic , Mitochondria/pathology , Mutagenesis/genetics , Oxidants/pharmacology , Purine Nucleosides/biosynthesis , Reactive Oxygen Species/chemistry , Superoxides/metabolism
5.
Anal Chem ; 89(2): 1299-1306, 2017 01 17.
Article in English | MEDLINE | ID: mdl-27982582

ABSTRACT

Post-translational modifications (PTMs) affect protein function, localization, and stability, yet very little is known about the ratios of these modifications. Here, we describe a novel method to quantitate and assess the relative stoichiometry of Lys and Arg modifications (QuARKMod) in complex biological settings. We demonstrate the versatility of this platform in monitoring recombinant protein modification of peptide substrates, PTMs of individual histones, and the relative abundance of these PTMs as a function of subcellular location. Lastly, we describe a product ion scanning technique that offers the potential to discover unexpected and possibly novel Lys and Arg modifications. In summary, this approach yields accurate quantitation and discovery of protein PTMs in complex biological systems without the requirement of high mass accuracy instrumentation.


Subject(s)
Arginine/analysis , Chromatography, High Pressure Liquid/methods , Histones/chemistry , Lysine/analysis , Peptides/chemistry , Protein Processing, Post-Translational , Tandem Mass Spectrometry/methods , HEK293 Cells , Humans , Hydrolysis , Jumonji Domain-Containing Histone Demethylases/chemistry , Recombinant Proteins/chemistry
6.
Chem Res Toxicol ; 28(12): 2334-42, 2015 Dec 21.
Article in English | MEDLINE | ID: mdl-26469224

ABSTRACT

Chronic inflammation results in increased production of reactive oxygen species (ROS), which can oxidize cellular molecules including lipids and DNA. Our laboratory has shown that 3-(2-deoxy-ß-d-erythro-pentofuranosyl)pyrimido[1,2-α]purin-10(3H)-one (M1dG) is the most abundant DNA adduct formed from the lipid peroxidation product, malondialdehyde, or the DNA peroxidation product, base propenal. M1dG is mutagenic in bacterial and mammalian cells and is repaired via the nucleotide excision repair system. Here, we report that M1dG levels in intact DNA were increased from basal levels of 1 adduct per 10(8) nucleotides to 2 adducts per 10(6) nucleotides following adenine propenal treatment of RKO, HEK293, or HepG2 cells. We also found that M1dG in genomic DNA was oxidized in a time-dependent fashion to a single product, 6-oxo-M1dG (to ∼ 5 adducts per 10(7) nucleotides), and that this oxidation correlated with a decline in M1dG levels. Investigations in RAW264.7 macrophages indicate the presence of high basal levels of M1dG (1 adduct per 10(6) nucleotides) and the endogenous formation of 6-oxo-M1dG. This is the first report of the production of 6-oxo-M1dG in genomic DNA in intact cells, and it has significant implications for understanding the role of inflammation in DNA damage, mutagenesis, and repair.


Subject(s)
DNA Adducts/chemistry , Purine Nucleosides/chemistry , Adenine/analogs & derivatives , Adenine/toxicity , Animals , Cell Nucleus/genetics , Cells, Cultured , Chromatography, Liquid , HEK293 Cells , Humans , Lipid Peroxidation , Macrophages/drug effects , Mass Spectrometry , Oxidation-Reduction
7.
Chem Res Toxicol ; 27(10): 1732-42, 2014 Oct 20.
Article in English | MEDLINE | ID: mdl-25211669

ABSTRACT

Base propenals are products of the reaction of DNA with oxidants such as peroxynitrite and bleomycin. The most reactive base propenal, adenine propenal, is mutagenic in Escherichia coli and reacts with DNA to form covalent adducts; however, the reaction of adenine propenal with protein has not yet been investigated. A survey of the reaction of adenine propenal with amino acids revealed that lysine and cysteine form adducts, whereas histidine and arginine do not. N(ε)-Oxopropenyllysine, a lysine-lysine cross-link, and S-oxopropenyl cysteine are the major products. Comprehensive profiling of the reaction of adenine propenal with human serum albumin and the DNA repair protein, XPA, revealed that the only stable adduct is N(ε)-oxopropenyllysine. The most reactive sites for modification in human albumin are K190 and K351. Three sites of modification of XPA are in the DNA-binding domain, and two sites are subject to regulatory acetylation. Modification by adenine propenal dramatically reduces XPA's ability to bind to a DNA substrate.


Subject(s)
Adenine/analogs & derivatives , Serum Albumin/chemistry , Xeroderma Pigmentosum Group A Protein/chemistry , Adenine/chemistry , Amino Acid Sequence , Chromatography, High Pressure Liquid , Cysteine/chemistry , Fluorescence Polarization , Humans , Lysine/chemistry , Molecular Sequence Data , Peptides/analysis , Peptides/chemistry , Tandem Mass Spectrometry
8.
Bioorg Med Chem ; 20(9): 3009-15, 2012 May 01.
Article in English | MEDLINE | ID: mdl-22464686

ABSTRACT

Introducing structural diversity into the nucleoside scaffold for use as potential chemotherapeutics has long been considered an important approach to drug design. In that regard, we have designed and synthesized a number of innovative 2'-deoxy expanded nucleosides where a heteroaromatic thiophene spacer ring has been inserted in between the imidazole and pyrimidine ring systems of the natural purine scaffold. The synthetic efforts towards realizing the expanded 2'-deoxy-guanosine and -adenosine tricyclic analogues as well as the preliminary biological results are presented herein.


Subject(s)
Antineoplastic Agents/chemical synthesis , Antiviral Agents/chemical synthesis , Purine Nucleosides/chemistry , Antineoplastic Agents/chemistry , Antineoplastic Agents/pharmacology , Antiviral Agents/chemistry , Antiviral Agents/pharmacology , Cell Line, Tumor , Cell Proliferation/drug effects , Drug Design , Guanosine/chemistry , HeLa Cells , Humans , Imidazoles/chemistry , Purine Nucleosides/chemical synthesis , Purine Nucleosides/pharmacology , Pyrimidines/chemistry , Thiophenes/chemistry , Virus Replication/drug effects
9.
Synthesis (Stuttg) ; 44(22): 3496-3504, 2012.
Article in English | MEDLINE | ID: mdl-24465059

ABSTRACT

Two series of innovative 2'-deoxy nucleoside analogues have been designed where the nucleobase has been split into its imidazole and pyrimidine subunits. This structural modification serves to introduce flexibility into the nucleobase scaffold while still retaining the elements required for recognition. The synthetic efforts to realize these analogues are described within.

10.
Biochemistry ; 50(48): 10540-9, 2011 Dec 06.
Article in English | MEDLINE | ID: mdl-22032234

ABSTRACT

AP endonuclease 1 (APE1) is a multifaceted protein with essential roles in DNA repair and transcriptional regulation. APE1 (ref-1) activates many transcription factors (TF), including AP-1 and NF-κB. While the mechanism of APE1 redox activity remains unknown, it may involve reduction of an oxidized Cys in the TF DNA-binding domain. Several small molecules inhibit APE1-mediated TF activation, including the quinone derivative E3330. It has been proposed some inhibitors bind near C65, a residue suggested to be important for TF activation, but the binding site has not been determined for any inhibitor. Remarkably, NMR and molecular docking studies here reveal E3330 binds in the DNA repair active site of APE1, far removed from C65. Accordingly, AP endonuclease activity is substantially inhibited by E3330 (100 µM), suggesting that E3330 may not selectively inhibit APE1 redox activity in cells, in contrast with previous proposals. A naphthoquinone analogue of E3330, RN7-60, binds a site removed from both C65 and the repair active site. While a detailed understanding of how these inhibitors work requires further studies into the mechanism of redox activity, our results do not support proposals that E3330 binds selectively (and slowly) to locally unfolded APE1 or that E3330 promotes formation of disulfide bonds in APE1. Rather, we suggest E3330 may suppress a conformational change needed for redox activity, disrupt productive APE1-TF binding, or block the proposed redox chaperone activity of APE1. Our results provide the first structural information for any APE1 redox inhibitor and could facilitate development of improved inhibitors for research and perhaps clinical purposes.


Subject(s)
Benzoquinones/chemistry , Benzoquinones/metabolism , DNA-(Apurinic or Apyrimidinic Site) Lyase/chemistry , DNA-(Apurinic or Apyrimidinic Site) Lyase/metabolism , Enzyme Inhibitors/chemistry , Enzyme Inhibitors/metabolism , Methacrylates/chemical synthesis , Methacrylates/metabolism , Naphthoquinones/chemical synthesis , Naphthoquinones/metabolism , Propionates/chemistry , Propionates/metabolism , Animals , Benzoquinones/pharmacology , Binding Sites/drug effects , DNA Repair/drug effects , DNA-(Apurinic or Apyrimidinic Site) Lyase/antagonists & inhibitors , Enzyme Inhibitors/pharmacology , Humans , Magnetic Resonance Spectroscopy , Methacrylates/pharmacology , Mice , Naphthoquinones/pharmacology , Oxidation-Reduction/drug effects , Propionates/pharmacology , Protein Binding/drug effects
11.
Org Lett ; 12(20): 4466-9, 2010 Oct 15.
Article in English | MEDLINE | ID: mdl-20845910

ABSTRACT

Promising biological activity in a number of therapeutic areas has been reported for both tricyclic nucleosides and 2'-modified nucleosides. In particular, disubstitution at the C-2' position of nucleosides has resulted in significant activity against the hepatitis C virus (HCV). Combining this with the observation that tricyclic nucleosides developed in our laboratory have been shown to inhibit the RNA-dependent RNA polymerase NS5B led to the design of a series of 2'-modified tricyclic nucleosides. Details of the synthesis, structural characterization, and preliminary biological results are reported.


Subject(s)
Antiviral Agents/chemical synthesis , Hepacivirus/drug effects , Heterocyclic Compounds, 3-Ring/chemical synthesis , Nucleosides/chemical synthesis , Molecular Structure
12.
Nucleic Acids Symp Ser (Oxf) ; (52): 635-6, 2008.
Article in English | MEDLINE | ID: mdl-18776540

ABSTRACT

Several thieno-expanded purine nucleoside analogues were synthesized for use as tools in ongoing investigations into nucleic acid structure and function in our laboratories. The inclusion of the thiophene ring system in the nucleoside endows the purine scaffold with advantages not previously available in other reported expanded purines. The synthesis and preliminary biological studies are reported herein.


Subject(s)
Enzyme Inhibitors/chemical synthesis , Purine Nucleosides/chemical synthesis , Purine Nucleosides/pharmacology , RNA-Dependent RNA Polymerase/antagonists & inhibitors , Drug Design , Enzyme Inhibitors/chemistry , Enzyme Inhibitors/pharmacology , Purine Nucleosides/chemistry , RNA-Dependent RNA Polymerase/metabolism
13.
Tetrahedron ; 64(48): 10791-10797, 2008 Nov 24.
Article in English | MEDLINE | ID: mdl-19946353

ABSTRACT

During the synthetic pursuit of guanosine (triG) and xanthosine (triX) tricyclic nucleosides analogues, an interesting side product was discovered. In an effort to uncover the mechanistic factors leading to this result, a series of reaction conditions were investigated. It was found that by varying the conditions, the appearance of the side product could be controlled. In addition, the yield of the desired products could be manipulated to afford either a 50:50 mix of both triG and triX, or a majority of one or the other. To demonstrate the broad utility of the method, it was also adapted to the synthesis of guanosine and xanthosine from 5-amino-1-beta-D-ribofuranosyl-4-imidazolecarboxyamide (AICAR). The mechanistic details surrounding the synthetic efforts are reported herein.

14.
Mol Divers ; 11(3-4): 115-8, 2007.
Article in English | MEDLINE | ID: mdl-18027097

ABSTRACT

This paper examines ring size patterns of natural product macrocycles. Evidence is presented that natural macrocycles containing 14-, 16-, and 18-membered rings are of frequent occurrence based on a data mining study. The results raise a question about the limited diversity of macrocycle ring sizes and the nature of the constraints that may cause them. The data suggest that the preference bears no relationship to the odd-even frequency in natural fatty acids. The trends reported here, along with those reported previously (Wessjohann et al. (2005) Mol Divers 9:171), may be generalized to better understand the possible structure preferences of natural macrocycles.


Subject(s)
Biological Products/chemistry , Macrocyclic Compounds/chemistry , Cyclization , Fatty Acids/chemistry , Molecular Structure , Oceans and Seas
15.
J Nat Prod ; 67(7): 1141-6, 2004 Jul.
Article in English | MEDLINE | ID: mdl-15270568

ABSTRACT

A literature survey and theoretical calculations have been applied to explore bilateral symmetry in natural product systems. Molecular bilateral symmetry is defined to include C(2) (sigma plane or axis), C(s)(), and C(2)(v)() point groups in molecules. Natural products that possess chirality in the form of C(2)-axes or sigma planes of symmetry are present in higher proportions (69%) compared to molecules bearing achiral C(s)() or C(2)(v)() point groups (14% and 16%, respectively). Density functional theoretical and semiempirical calculations indicate that the dimers 3,3'-dibromo-5,5'-[N-(2-(3-bromo-4-hydroxyphenyl)ethyl)-2-hydroxyiminoacetamide]biphenyl-2,2'-diol (1), (S,S)-1,2-bis(2-amino-3H-imidazol-4-yl)-(R,R)-3,4-bis(1H-pyrrole-2-amido)cyclobutane (2), 2-oxo-dimethyl-1,3-bis(3,4-dibromobenzene-1,2-diol) (11), 1,7-bis(4-hydroxy-3-methoxyphenyl)hepta-1,6-diene-3,5-dione (12), and bis(5-isopropyl-8-methylazulene)methane (13) evolve more energy per connecting bond than the corresponding trimers or tetramers would. This we propose is a guiding parameter that may adjust molecule growth. The corresponding trimers, tetramers, or higher oligomers of 1, 2, and 11-13 appear to represent "missing" compounds in nature. Natural products 1, 2, and 11-13, having 3-fold and higher levels of symmetry, would founder on the lack of a facile method of synthesis and on the prohibitively high-energy costs caused by steric crowding at their core.


Subject(s)
Biological Products/chemistry , Models, Molecular , Molecular Structure , Stereoisomerism , Structure-Activity Relationship
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