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1.
Clin Genet ; 77(6): 563-71, 2010 Jun.
Article in English | MEDLINE | ID: mdl-20132242

ABSTRACT

Myosin VIIA mutations have been associated with non-syndromic hearing loss (DFNB2; DFNA11) and Usher syndrome type 1B (USH1B). We report clinical and genetic analyses of a consanguineous Iranian family segregating autosomal recessive non-syndromic hearing loss (ARNSHL). The hearing impairment was mapped to the DFNB2 locus using Affymetrix 50K GeneChips; direct sequencing of the MYO7A gene was completed. The Iranian family (L-1419) was shown to segregate a novel homozygous missense mutation (c.1184G>A) that results in a p.R395H amino acid substitution in the motor domain of the myosin VIIA protein. As one affected family member had significantly less severe hearing loss, we used a candidate approach to search for a genetic modifier. This novel MYO7A mutation is the first reported to cause DFNB2 in the Iranian population and this DFNB2 family is the first to be associated with a potential modifier. The absence of vestibular and retinal defects, and less severe low frequency hearing loss, is consistent with the phenotype of a recently reported Pakistani DFNB2 family. Thus, we conclude this family has non-syndromic hearing loss (DFNB2) rather than USH1B, providing further evidence that these two diseases represent discrete disorders.


Subject(s)
Hearing Loss/genetics , Mutation, Missense , Myosins/genetics , Adult , Amino Acid Sequence , Base Sequence , Chromosome Mapping , Consanguinity , Family , Female , Hearing Loss, Sensorineural/genetics , Humans , Male , Middle Aged , Molecular Sequence Data , Myosin VIIa
2.
Breast Cancer Res Treat ; 123(2): 397-404, 2010 Sep.
Article in English | MEDLINE | ID: mdl-19949854

ABSTRACT

Recent advances in the study of the tumor microenvironment have revealed significant interaction between tumor cells and their surrounding stroma in model systems. We have previously shown that two distinct stromal signatures derived from a macrophage (CSF1) response and a fibroblastic (DTF-like) response are present in subsets of invasive breast cancers and show a correlation with clinical outcome. In the present study we explore whether these signatures also exist in the stroma of ductal carcinoma in situ (DCIS). We studied the signatures by both gene expression profile analysis of a publically available data set of DCIS and by immunohistochemistry (IHC) on a tissue microarray of DCIS and invasive breast cancer cases. Both the gene expression and immunohistochemical data show that the macrophage response and fibroblast expression signatures are present in the stroma of subsets of DCIS cases. The incidence of the stromal signatures in DCIS is similar to the incidence in invasive breast cancer that we have previously reported. We also find that the macrophage response signature is associated with higher grade DCIS and cases which are ER and PR negative, whereas the fibroblast signature was not associated with any clinicopathologic features in DCIS. A comparison of 115 matched cases of DCIS and invasive breast cancer found a correlation between the type of stromal response in DCIS and invasive ductal carcinoma (IDC) within the same patient for both the macrophage response and the fibroblast stromal signatures (P = 0.03 and 0.08, respectively). This study is a first characterization of these signatures in DCIS. These signatures have significant clinicopathologic associations and tend to be conserved as the tumor progresses from DCIS to invasive breast cancer.


Subject(s)
Biomarkers, Tumor/analysis , Breast Neoplasms/chemistry , Breast Neoplasms/genetics , Carcinoma, Ductal, Breast/chemistry , Carcinoma, Ductal, Breast/genetics , Carcinoma, Intraductal, Noninfiltrating/chemistry , Carcinoma, Intraductal, Noninfiltrating/genetics , Stromal Cells/chemistry , Biomarkers, Tumor/genetics , Breast Neoplasms/pathology , Carcinoma, Ductal, Breast/pathology , Carcinoma, Intraductal, Noninfiltrating/pathology , Disease Progression , Female , Fibroblasts/chemistry , Gene Expression Profiling/methods , Gene Expression Regulation, Neoplastic , Genotype , Humans , Immunohistochemistry , Macrophages/chemistry , Neoplasm Invasiveness , Neoplasm Staging , Oligonucleotide Array Sequence Analysis , Phenotype , Stromal Cells/pathology , Tissue Array Analysis
3.
J Thorac Cardiovasc Surg ; 121(4 Suppl): S8-11, 2001 Apr.
Article in English | MEDLINE | ID: mdl-11279438

ABSTRACT

Building a multi-institutional cardiothoracic surgical program has the same guiding principles and values as a traditional single institutional program: ensuring high-quality patient care, training and fostering residents, recruiting and retaining quality faculty, and contributing to basic and clinical research. With a well-designed infrastructure and support system, this more complicated type of organization may permit academic cardiothoracic surgical programs to compete effectively and grow in a constantly changing economic and political environment.


Subject(s)
Education, Medical, Graduate , Internship and Residency , Multi-Institutional Systems/organization & administration , Thoracic Surgery/education , Thoracic Surgery/organization & administration , Humans , Program Development
4.
J Econ Entomol ; 93(4): 1315-8, 2000 Aug.
Article in English | MEDLINE | ID: mdl-10985048

ABSTRACT

Durable resistance to greenbug, Schizaphis graminum (Rondani), in wheat is a goal of wheat improvement teams, and one that has been complicated by the regular occurrence of damaging biotypes. Simulation modeling studies suggest that pyramiding resistance genes, i.e., combining more than one resistance gene in a single cultivar or hybrid, may provide more durable resistance than sequential releases of single genes. We examined this theory by pyramiding resistance genes in wheat and testing a series of greenbug biotypes. Resistance genes Gb2, Gb3, and Gb6, and pyramided genes Gb2/Gb3, Gb2/Gb6, and Gb3/Gb6 were tested for effectiveness against biotypes E, F, G, H, and I. By comparing reactions of plants with pyramided genes to those with single resistance genes, we found that pyramiding provided no additional protection over that conferred by the single resistance genes. Based on the results of this test, we concluded that the sequential release of single resistance genes, combined with careful monitoring of greenbug population biotypes, is the most effective gene deployment strategy for greenbug resistance in wheat.


Subject(s)
Aphids/physiology , Genes, Plant , Pest Control, Biological , Triticum/genetics , Animals
5.
J Econ Entomol ; 93(3): 1000-4, 2000 Jun.
Article in English | MEDLINE | ID: mdl-10902362

ABSTRACT

Several biotypes of the greenbug, Schizaphis graminum (Rondani), attack winter wheat, Triticum aestivum L., on the Southern Plains every year. Two wheat germplasm sources of resistance ('Largo' and 'GRS 1201') have been developed that provide protection against the three predominant greenbug biotypes (E, I, and K). Each source has agronomic and end-use quality advantages and disadvantages for the breeder to consider in choosing a greenbug-resistant breeding line. We compared these two germplasms to determine their levels of resistance against biotype E. Components of resistance (i.e., antibiosis, antixenosis, and tolerance) were measured on seedlings of GRS 1201, Largo, and 'TAM W-101' (a susceptible control). Several aphid and plant measurements (e.g., total number of aphids produced per plant, aphid selection preferences, and plant damage ratings) were recorded for each plant entry. Select data recorded for each resistance component were normalized and combined to derive a plant resistance index for each wheat entry. Results indicated that GRS 1201 had a higher level of combined resistance components than did Largo, followed by TAM W-101, the susceptible control. These data provide additional information for the breeder to consider in selecting a greenbug-resistant breeding line.


Subject(s)
Aphids , Insect Control/methods , Triticum/physiology , Animals
6.
Int J Syst Bacteriol ; 49 Pt 2: 489-502, 1999 Apr.
Article in English | MEDLINE | ID: mdl-10319469

ABSTRACT

In accordance with Recommendation 30b of the International Code of Nomenclature of Bacteria, minimal standards are proposed for the genus Staphylococcus and the description of newly recognized species in this genus. Assignment of a strain to the genus Staphylococcus requires that it is a Gram-positive coccus that forms clusters, produces catalase, has an appropriate cell wall structure (including peptidoglycan type and teichoic acid presence) and G + C content of DNA in a range of 30-40 mol%. The recommended minimal standards for describing a new Staphylococcus species are based on the results of phenotypic and genomic studies of at least five independently isolated strains. They include colony morphology and the results of the following conventional tests: pigment production, growth requirements, fermentative and oxidative activity on carbohydrates, novobiocin susceptibility, enzymic activities (nitrate reductase, alkaline phosphatase, arginine dihydrolase, ornithine decarboxylase, urease, cytochrome oxidase, staphylocoagulase in rabbit plasma, heat-stable nuclease, amidases, oxidases, clumping factor, and haemolytic activity on sheep or bovine blood agar). DNA-DNA hybridization experiments may distinguish species when the difference between the binding in the homologous reaction and the binding in the heterologous reaction expressed as a percentage is less than 70%. In addition, rRNA signature sequence criteria, ribotyping characterization of the nomenclature type strain and other strains of the species, and reference strains of other species is recommended to describe the strains of the new species with sets of genetic attributes and reveal possible grouping errors. This proposal has been endorsed by the members of the Subcommittee on the taxonomy of staphylococci and streptococci of the international Committee on Systematic Bacteriology.


Subject(s)
Bacterial Typing Techniques , Staphylococcus/classification , Staphylococcus/genetics , Terminology as Topic , Animals , Cattle , Genotype , Phenotype , Rabbits , Species Specificity , Staphylococcus/cytology , Staphylococcus/physiology
7.
Int J Syst Bacteriol ; 48 Pt 3: 859-77, 1998 Jul.
Article in English | MEDLINE | ID: mdl-9734040

ABSTRACT

Four species of the newly proposed genus Macrococcus, namely macrococcus caseolyticus gen. nov., comb. nov. (formerly Staphylococcus caseolyticus Schleifer, Kilpper-Bälz, Fischer, Faller and Endl 1982, 19VP), Macrococcus equipercicus sp. nov., Macrococcus bovicus sp. nov. Macrococcus carouselicus sp. nov., are described on the basis of a phylogenetic analysis comparing 16S rRNA sequences, DNA-DNA liquid hybridization, DNA base composition, normalized ribotype patterns, macrorestriction pattern analysis and estimation of genome size using PFGE, cell wall composition, phenotypic characteristics and plasmid profiles. Compared with their closet relatives, members of the genus Staphylococcus, these organisms demonstrated significantly lower 16S rRNA sequence similarities (93.4-95.3%), higher DNA G+C content (38-45 mol%), absence of cell wall teichoic acids (with the possible exception of M. caseolyticus), unique ribotype pattern types and macrorestriction patterns, smaller genome size (approx. 1500-1800 kb) and generally larger Gram-stained cell size (1.1-2.5% microns in diameter). Macrococci can be distinguished from most species of staphylococci (except Staphylococcus sciuri, Staphylococcus vitulus and Staphylococcus lentus) by thier oxidase activity. The four Macrococcus species can be distinguished from one another on the basis of DNA-DNA hybridization, ribotype pattern types, macrorestriction patterns and their phenotypic properties, including colony morphology, cell morphology, haemolysins, Staphy Latex agglutination, acid production from a variety of carbohydrates, acetoin production, nitrate reduction, aesculin hydrolysis, and DNase and urease activities. The type species is M. equipercicus. The type strains of M. equipercicus, M. caseolyticus, M. bovicus and M. carouselicus are ATTCC 51831T (= DD 9350T) ATCC 13548T (= TDD 4508T) (Schleifer et al. 1982, ATCC 51825T (= DD 4516T) and ATCC 51828T (= DD 9348), respectively.


Subject(s)
Micrococcaceae/classification , Staphylococcus/classification , Animals , Base Composition , Base Sequence , Cell Wall/chemistry , DNA, Ribosomal/chemistry , Horses , Molecular Sequence Data , Nucleic Acid Hybridization , Phenotype , Phylogeny , Plasmids , RNA, Ribosomal, 16S/genetics
8.
J Behav Med ; 21(1): 83-102, 1998 Feb.
Article in English | MEDLINE | ID: mdl-9547423

ABSTRACT

This study examined the relative impact of different self-reward strategies on maintenance of breast self-examination (BSE) practice among 1649 women trained to do BSE. Training groups were randomized into four conditions: (a) self-reward instructions and materials delivered at the end of the BSE training session; (b) self-reward suggestions delivered through the mail each month, contingent upon the BSE performance; (c) external monetary rewards and self-reward suggestions delivered through the mail each month on an intermittent schedule, contingent upon BSE practice; and (d) a no-reward control condition. Follow-up assessments 12 months following training revealed a pattern of evidence in support of the benefits of external monetary rewards and self-reward prompts on BSE frequency and quality; however, it is likely that the value of that condition lies in the external reward component.


Subject(s)
Breast Self-Examination/psychology , Motivation , Personality Assessment , Reward , Adolescent , Adult , Aged , Female , Follow-Up Studies , Health Education , Humans , Internal-External Control , Middle Aged
9.
Drug Dev Ind Pharm ; 24(4): 395-9, 1998 Apr.
Article in English | MEDLINE | ID: mdl-9876601

ABSTRACT

The in vitro release characteristics of amoxycillin from different lipophilic suppository bases were investigated using the USP rotating basket method. Suppositories containing 250 mg amoxycillin were prepared in theobroma oil and in the semi-synthetic bases Witepsol W35, Suppocire A32, Novata BD, and Novata 299. Both freshly prepared and 1-month-old suppositories were tested. Analysis of amoxycillin was performed using a validated high-performance liquid chromatographic (HPLC) technique. Release profiles differed significantly between bases, with the greatest amount of amoxycillin being released from both newly made and 1-month-old Novata BD bases (87.57 +/- 8.18 and 99.66 +/- 6.63%, respectively), and the lowest amount released from the newly manufactured theobroma suppositories (8.82 +/- 0.75%) and the 1-month-old Suppocire A32 suppositories (7.78 +/- 0.27%).


Subject(s)
Amoxicillin/administration & dosage , Amoxicillin/pharmacokinetics , Penicillins/administration & dosage , Penicillins/pharmacokinetics , Amoxicillin/isolation & purification , Biological Availability , Chromatography, High Pressure Liquid , Drug Compounding/methods , Humans , In Vitro Techniques , Lipids , Penicillins/isolation & purification , Pharmaceutical Vehicles , Suppositories
10.
Insect Mol Biol ; 6(3): 203-9, 1997 Aug.
Article in English | MEDLINE | ID: mdl-9272438

ABSTRACT

In the United States, the greenbug, Schizaphis graminum (Rondani), reproduces primarily by apomictic parthenogenesis. Although a periodic sexual cycle exists, the extent to which it occurs naturally and its influence on the genetic variability of greenbug populations is unclear. Length variation in the intergenic spacer (IGS) of the rRNA cistron in the greenbug indicates that populations are made up of many genetically distinct clones. Previous laboratory studies have shown the stability of the IGS within parthenogenetic clones. By inducing the sexual reproductive cycle of the greenbug, we conducted both Intra- and inter-clone matings and studied the inheritance of the IGS in the offspring. In both mating schemes, rearrangements in the IGS were apparent. IGS diversity found among the offspring could be attributed to unequal cross-over and probably other molecular drive events during meiosis. Periodic sexual reproduction is a primary mechanism for the generation and maintenance of genetic variability in greenbug populations, and explains the level of clonal diversity found in previous studies.


Subject(s)
Aphids/genetics , DNA, Ribosomal/genetics , Genetic Variation/genetics , Animals , Aphids/physiology , Crosses, Genetic , Female , Male , Reproduction
11.
Int J Syst Bacteriol ; 47(2): 313-23, 1997 Apr.
Article in English | MEDLINE | ID: mdl-9103615

ABSTRACT

Three subspecies of Staphylococcus sciuri, S. sciuri subsp. sciuri Kloos, Schleifer, and Smith 1976, 23AL emend. Kloos et al. 1997 [corrected], S. sciuri subsp. carnaticus subsp. nov., and S. sciuri subsp. rodentium subsp. nov., are described on the basis of their ribotype patterns, DNA-DNA liquid hybridization data, and phenotypic characteristics. Normalized ribotyping subdivided the S. sciuri patterns into three blocks of patterns, each corresponding to a subspecies. Each subspecies formed a separate, well-defined DNA similarity group when DNA-DNA hybridizations were conducted under stringent (70 degrees C) reassociation conditions. S. sciuri subsp. sciuri could be distinguished from the other subspecies on the basis of its ability to produce acid from D-cellobiose, alkaline phosphatase activity, and inability to produce either clumping factor or protein A. S. sciuri subsp. carnaticus could be distinguished by its ability to produce acid aerobically from D-xylose and maltose, inability to produce acid from D-melezitose, and smaller colony size on P agar. S. sciuri subsp. rodentium could be distinguished by its positive reaction in the latex agglutination test for clumping factor and/or protein A and generally higher frequencies and levels of oxacillin and methicillin resistance. All 40 strains of S. sciuri tested (including representatives of all three subspecies) hybridized with the mecA gene probe. All strains of S. sciuri subsp. sciuri, 79% of the strains of S. sciuri subsp. carnaticus and 89% of the strains of S. sciuri subsp. rodentium exhibited extracellular, staphylolytic enzyme activity. This activity was associated with an enzyme(s) that immunoblotted with a lysostaphin-specific monoclonal antibody; however, only three strains hybridized with a lysostaphin (end) gene probe. The type strain of S. sciuri subsp. carnaticus is DD 791 (= ATCC 700058), and the type strain of S. sciuri subsp. rodentium is DD 4761 (= ATCC 700061).


Subject(s)
Staphylococcus/classification , Bacterial Typing Techniques , Base Composition , Cell Wall/chemistry , DNA, Bacterial/chemistry , DNA, Bacterial/genetics , Enzymes/genetics , Genes, Bacterial , Methicillin Resistance/genetics , Nucleic Acid Hybridization , Phenotype , Species Specificity , Staphylococcus/drug effects , Staphylococcus/genetics
12.
Int J Food Microbiol ; 32(3): 263-78, 1996 Oct.
Article in English | MEDLINE | ID: mdl-8913799

ABSTRACT

Seven laboratories participated in a WHO-sponsored international collaborative study, to evaluate methods for subtyping Listeria monocytogenes, by performing restriction fragment length polymorph sm (RFLP) analysis-based subtyping of an international study set of 80 strains of L. monocytogenes that included 22 epidemiologically related groups. The RFLP analysis was done by Southern hybridization with one of two types of probes found in multiple copies on the chromosome of L. monocytogenes. Six laboratories performed ribotyping. These laboratories used EcoRI enzyme to restrict the L. monocytogenes DNA and ribosomal RNA or DNA as the probe for Southern hybridizations. The seventh laboratory used Ncil to restrict the DNA, and two probes, one randomly cloned and the other containing repeat sequences cloned from L. monocytogenes DNA. The overall discriminating power of ribotyping, as estimated by calculation of Simpson's index of diversity, ranged from 0.83 to 0.88 for the six laboratories. The discriminating power of the combination of two probes used by Laboratory 7 was 0.91. Ribotyping and the cloned probes used by Laboratory 7 discriminated poorly between serotype 4b strains. Neither method identified three atypical strains (identified by other subtyping methods) included in three apparently epidemiologically related groups. Ribotyping did not discriminate between strains of serotypes 4b and 4b(X) in one epidemiologically related group of strains; one cloned probe used by Laboratory 7 discriminated between these strains. Intra-laboratory reproducibilities for the seven laboratories ranged from 80.0 to 100%. as determined by their abilities to correctly identify 11 pairs of duplicate strains included in the study set. Inter-laboratory reproducibilities were generally very good considering that no attempt was made to standardize protocols used by the participants.


Subject(s)
Bacterial Typing Techniques , Listeria monocytogenes/classification , Blotting, Southern , Chromosomes, Bacterial , DNA, Ribosomal/analysis , Listeria monocytogenes/genetics , Polymorphism, Restriction Fragment Length , RNA, Ribosomal, 16S/genetics , RNA, Ribosomal, 23S/genetics , World Health Organization
13.
J Athl Train ; 31(2): 179-81, 1996 Apr.
Article in English | MEDLINE | ID: mdl-16558395
14.
Microb Drug Resist ; 2(4): 377-91, 1996.
Article in English | MEDLINE | ID: mdl-9158808

ABSTRACT

In an effort to explore the origin and/or reservoirs of the genetic determinant(s) of methicillin resistance in Staphylococcus aureus, we examined over 200 strains representing 13 different species within the genus Staphylococcus for the presence of the mecA gene, using a DNA probe internal to this gene prepared from a methicillin-resistant strain of S. aureus. Occasional mecA- positive isolates were detected among several staphylococcal species. On the other hand, each one of the 134 isolates of Staphylococcus sciuri, a species considered taxonomically the most primitive among staphylococci and found primarily on rodents and primitive mammals, gave positive reaction with the DNA probe when tested under conditions of high stringency. About two thirds (99) of these isolates, all of which belonged to S. sciuri subspecies "sciuri," as well as 9 of the 11 species carnaticum isolates, showed only marginal, if any, resistance to methicillin (minimal inhibitory concentration of 0.75-6.0 micrograms/ml), while most of the remaining isolates that belonged to the subspecies "rodentius" (13 isolates in all) expressed antibiotic resistance with a heterogeneous phenotype similar to those seen in many methicillin-resistance strains of S. aureus In SmaI digests of chromosomal DNA isolated from such "methicillin-resistant S. aureus-like" strains, the mecA probe hybridized with DNA fragments in the range of 145-180 kb, while in subspecies "sciuri" and carnaticum isolates the mecA hybridizing fragment was located in the SmaI fragment with the highest molecular size (> or = 400 kb). A DNA probe comprising an internal sequence to the regulatory gene mecI from Staphylococcus epidermidis identified the presence of sequences with low degree of homology in isolates of the three S. sciuri subspecies. The mecA-reacting sequences in these bacteria differed from mecA of S. aureus in several respects (e.g., by the absence of a ClaI restriction site from mecA of subspecies "sciuri" and carnaticum, and in some isolates of subspecies "rodentius." The uniform presence of mecA in each one of a large number of S. sciuri strains belonging to distinct ribotypes and macrorestriction patterns and recovered over a 20-year period from a wide variety of animal sources and geographic sites suggests that mecA may be a native genetic element with an as yet unidentified physiologic function in this staphylococcal species.


Subject(s)
Bacterial Proteins/genetics , Genes, Bacterial/genetics , Staphylococcus/metabolism , Animals , DNA Probes , Electrophoresis, Polyacrylamide Gel , Genotype , Immunoblotting , In Situ Hybridization , Methicillin Resistance , Polymorphism, Genetic , Rodentia , Staphylococcus/genetics , beta-Lactamases/biosynthesis
15.
Proc Natl Acad Sci U S A ; 92(11): 5229-33, 1995 May 23.
Article in English | MEDLINE | ID: mdl-7539145

ABSTRACT

To classify Listeria monocytogenes using taxonomic characters derived from the rRNA operons and their flanking sequences, we studied a sample of 1346 strains within the taxon. DNA from each strain was digested with a restriction endonuclease, EcoRI. The fragments were separated by gel electrophoresis, immobilized on a membrane, and hybridized with a labeled rRNA operon from Escherichia coli. The pattern of bands, positions, and intensities of hybridized fragments were electronically captured. Software was used to normalize the band positions relative to standards, scale the signal intensity, and reduce the background so that each strain was reproducibly represented in a data base as a pattern. With these methods, L. monocytogenes was resolved into 50 pattern types differing in the length of at least one polymorphic fragment. Pattern types representing multiple strains were recorded as the mathematical average of the strain patterns. Pattern types were arranged by size polymorphisms of assigned rRNA regions into subsets, which revealed the branching genetic structure of the species. Subtracting the polymorphic variants of a specific assigned region from the pattern types and averaging the types within each subset resulted in reduced sets of conserved fragments that could be used to recognize strains of the species. Pattern types and reduced sets of conserved fragments were conserved among different strains of L. monocytogenes but were not observed in total among strains of other species.


Subject(s)
DNA, Bacterial/chemistry , DNA, Ribosomal/chemistry , Listeria monocytogenes/genetics , Base Sequence , Conserved Sequence , DNA, Bacterial/genetics , DNA, Ribosomal/genetics , Deoxyribonuclease EcoRI , Genes, Bacterial , Listeria monocytogenes/classification , Operon , RNA, Bacterial/chemistry , RNA, Bacterial/genetics , RNA, Ribosomal/biosynthesis , RNA, Ribosomal/genetics , Species Specificity
16.
Proc Natl Acad Sci U S A ; 92(11): 5234-8, 1995 May 23.
Article in English | MEDLINE | ID: mdl-7539146

ABSTRACT

By using taxonomic characters derived from EcoRI restriction endonuclease digestion of genomic DNA and hybridization with a labeled rRNA operon from Escherichia coli, a polymorphic structure of Listeria monocytogenes, characterized by fragments with different frequencies of occurrence, was observed. This structure was expanded by creating predicted patterns through a recursive process of observation, expectation, prediction, and assessment of completeness. This process was applied, in turn, to normalized strain patterns, fragment bands, and positions of EcoRI recognition sites relative to rRNA regions. Analysis of 1346 strains provided observed patterns, fragment sizes, and their frequencies of occurrence in the patterns. Fragment size statistics led to the creation of unobserved combinations of bands, predicted pattern types. The observed fragment bands revealed positions of EcoRI sites relative to rRNA sequences. Each EcoRI site had a frequency of occurrence, and unobserved fragment sizes were postulated on the basis of knowing the restriction site locations. The result of the recursion process applied to the components of the strain data was an extended classification with observed and predicted members.


Subject(s)
DNA, Ribosomal/genetics , Listeria monocytogenes/classification , Listeria monocytogenes/genetics , Polymorphism, Genetic , RNA, Ribosomal/genetics , Base Sequence , DNA, Bacterial/chemistry , DNA, Bacterial/genetics , DNA, Ribosomal/chemistry , Deoxyribonuclease EcoRI , Models, Theoretical , Probability , RNA, Bacterial/chemistry , RNA, Bacterial/genetics , RNA, Ribosomal/chemistry
17.
Int J Syst Bacteriol ; 44(3): 454-60, 1994 Jul.
Article in English | MEDLINE | ID: mdl-7520736

ABSTRACT

Strains of a new species, Staphylococcus vitulus, were isolated from food and a variety of mammals. This species was recognized on the basis of the results of an analysis of genomic EcoRI fragments containing portions of the rRNA operons. The patterns of hybridized fragments obtained from strains belonging to the new taxon were sorted into a distinguishable cluster and were distinct from the Staphylococcus lentus and Staphylococcus sciuri patterns. The results of DNA-DNA hybridization reactions demonstrated that strains in this cluster were more closely related to S. lentus and S. sciuri than to other Staphylococcus species and yet were significantly different. While these strains had some of the phenotypic characteristics of the S. sciuri species group, the newly recognized taxon could be distinguished by its very small colonies on P agar, absence of alkaline phosphatase activity, and lack of acid production from L-arabinose, maltose, N-acetylglucosamine, D-mannose, and raffinose. The type strain of the new species is strain DD 756 (= ATCC 51145).


Subject(s)
RNA, Bacterial/genetics , RNA, Ribosomal/genetics , Staphylococcus/classification , Staphylococcus/genetics , Animals , Base Composition , Cell Wall/chemistry , DNA, Bacterial/chemistry , DNA, Bacterial/genetics , DNA, Ribosomal/chemistry , DNA, Ribosomal/genetics , Deoxyribonuclease EcoRI , Food Microbiology , Humans , Mammals/microbiology , Nucleic Acid Hybridization , Operon , Peptidoglycan/analysis , Species Specificity , Staphylococcus/isolation & purification , Teichoic Acids/analysis
18.
Appl Environ Microbiol ; 59(4): 945-52, 1993 Apr.
Article in English | MEDLINE | ID: mdl-8476298

ABSTRACT

To facilitate genus and species level identification of a broad range of bacteria without the requirement of presumptive identification, we have developed a unified set of primers and polymerase chain reaction conditions to amplify spacer regions between the 16S and 23S genes in the prokaryotic rRNA genetic loci. Spacer regions within these loci show a significant level of length and sequence polymorphism across both genus and species lines. A generic pair of priming sequences was selected for the amplification of these polymorphisms from highly conserved sequences in the 16S and 23S genes occurring adjacent to these polymorphic regions. This single set of primers and reaction conditions was used for the amplification of 16S-23S spacer regions for over 300 strains of bacteria belonging to eight genera and 28 species or serotypes, including Listeria, Staphylococcus, and Salmonella species and additional species related to these pathogenic organisms. When the spacer amplification products were resolved by electrophoresis, the resulting patterns could be used to distinguish all of the species of bacteria within the test group. Unique elements in the amplification product patterns generally clustered at the species level, although some genus-specific characteristics were also observed. On the basis of the results obtained with our test group of 300 bacterial strains, amplification of the 16S-23S ribosomal spacer region is a suitable process for generating a data base for use in a polymerase chain reaction-based identification method, which can be comprehensively applied to the bacterial kingdom.


Subject(s)
Listeria/classification , RNA, Ribosomal, 16S/chemistry , RNA, Ribosomal, 23S/chemistry , RNA, Ribosomal, 5S/chemistry , Salmonella/classification , Sequence Analysis, RNA , Staphylococcus/classification , Base Sequence , Gene Amplification , Listeria/genetics , Molecular Sequence Data , Polymerase Chain Reaction/methods , Salmonella/genetics , Staphylococcus/genetics
19.
BMJ ; 305(6863): 1187-93, 1992 Nov 14.
Article in English | MEDLINE | ID: mdl-1467721

ABSTRACT

OBJECTIVE: To observe the effects of introducing an acute pain service to the general surgical wards of a large teaching hospital. DESIGN: A study in seven stages: (1) an audit of current hospital practice succeeded by the sequential introduction to the general surgical wards of (2) pain assessment charts; (3) an algorithm to allow more frequent use of intramuscular analgesia; (4) increased use of local anaesthetic techniques of wound infiltration and nerve blocks; (5) an information sheet for patients about postoperative pain; (6) the introduction of patient controlled analgesia; (7) a repeat audit of hospital practice. Data were collected on each patient 24 hours after operation. SETTING: University Hospital of Wales, which has both district general and tertiary referral functions. PATIENTS: 2035 patients over nine months from all surgical specialties (excluding cardiac) at the hospital. General surgical operations were studied in detail and separated into major, intermediate, and minor for data collection. MAIN OUTCOME MEASURES: A change in the median visual analogue pain scores 24 hours after surgery for pain during relaxation, pain on movement, and pain on deep inspiration at each stage of the study. RESULTS: There was a reduction in median visual analogue scores during the study. The median (95% confidence interval) scores for pain during relaxation decreased from 45 (34 to 53) in stage 1 to 16 (10 to 20) in stage 7 for major surgical procedures. Pain on movement decreased from 78 (66 to 80) to 46 (38 to 48), and pain on deep inspiration decreased from 64 (48 to 78) to 36 (31 to 38). The reductions in median scores for intermediate and minor operative procedures showed similar patterns. CONCLUSIONS: The introduction of an acute pain service to the general surgical wards led to considerable improvement in the level of postoperative pain as assessed by visual analogue scores. Simple techniques of regular pain assessment and the more frequent use of intramuscular analgesia as a result of using an algorithm were particularly effective.


Subject(s)
Hospital Units/organization & administration , Pain, Postoperative/therapy , Patient Care Team/organization & administration , Algorithms , Analgesia, Patient-Controlled , Clinical Protocols , Data Collection , Hospitals, University/organization & administration , Humans , Organizational Policy , Pain Measurement , Pain, Postoperative/etiology , Pain, Postoperative/prevention & control
20.
J Gerontol Nurs ; 14(12): 9-15, 1988 Dec.
Article in English | MEDLINE | ID: mdl-3204261
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