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1.
Nucleic Acids Res ; 30(20): 4452-9, 2002 Oct 15.
Article in English | MEDLINE | ID: mdl-12384592

ABSTRACT

We examined, by 1H and 31P NMR, the solution structure of a 16 bp non-palindromic DNA fragment (16M2) containing the HIV-1 NF-kappaB-binding site, in which the sequences flanking the kappaB site had been mutated. 31P NMR was particularly useful for obtaining structural information on the phosphodiester backbone conformation. Structural features were then compared with those of the two previously studied DNA fragments corresponding, respectively, to the native kappaB fragment (16N) and a fragment in which mutations have been introduced at the 5' end of the kappaB site (16M1). For the mutated 16M2 duplex, NMR data showed that the BI-BII equilibrium, previously reported for the native fragment (16N) at the kappaB flanking steps, was lost. The role of the BI-BII equilibrium in NF-kappaB recognition by DNA was then investigated by electrophoretic mobility shift assay. We found that the isolated kappaB site has the potential to bind efficiently due to the BI-BII equilibrium of the kappaB flanking sequences.


Subject(s)
DNA/chemistry , DNA/metabolism , NF-kappa B/metabolism , Phosphorus/analysis , Animals , Base Sequence , Binding Sites , Cell Line , Electrophoretic Mobility Shift Assay , Macromolecular Substances , Molecular Sequence Data , Nuclear Magnetic Resonance, Biomolecular , Nucleic Acid Conformation , Organophosphates/chemistry , Protein Binding
2.
Eur J Biochem ; 269(15): 3779-88, 2002 Aug.
Article in English | MEDLINE | ID: mdl-12153575

ABSTRACT

The human immunodeficiency virus type 1, HIV-1, genome encodes a highly conserved regulatory gene product, Vpr (96 amino acids), which is incorporated into virions in quantities equivalent to those of the viral Gag protein. In infected cells, Vpr is believed to function during the early stages of HIV-1 replication (such as transcription of the proviral genome and migration of preintegration nuclear complex), blocks cells in G2 phase and triggers apoptosis. Vpr also plays a critical role in long-term AIDS disease by inducing viral infection in nondividing cells such as monocytes and macrophages. To gain deeper insight of the structure-function relationship of Vpr, the intact protein (residues 1-96) was synthesized. Its three-dimensional structure was analysed using circular dichroism and two-dimensional 1H- and 15N-NMR and refined by restrained molecular dynamics. In addition, 15N relaxation parameters (T1, T2) and heteronuclear 1H-15N NOEs were measured. The structure of the protein is characterized by a well-defined gamma turn(14-16)-alpha helix(17-33)-turn(34-36), followed by a alpha helix(40-48)-loop(49-54)-alpha helix(55-83) domain and ends with a very flexible C-terminal sequence. This structural determination of the whole intact Vpr molecule provide insights into the biological role played by this protein during the virus life cycle, as such amphipathic helices are believed to be involved in protein-lipid bilayers, protein-protein and/or protein-nucleic acid interactions.


Subject(s)
Gene Products, vpr/chemistry , Amino Acid Sequence , Circular Dichroism , Magnetic Resonance Spectroscopy/methods , Models, Molecular , Molecular Sequence Data , Protein Conformation , Protein Structure, Tertiary , Solutions , Trifluoroethanol/chemistry , Water
3.
Eur J Biochem ; 266(2): 359-69, 1999 Dec.
Article in English | MEDLINE | ID: mdl-10561576

ABSTRACT

The human immunodeficiency virus type 1 (HIV-1) genome encodes a highly conserved 16 kDa regulatory gene product, Vpr (viral protein of regulation, 96 amino acid residues), which is incorporated into virions, in quantities equivalent to those of the viral Gag proteins. In the infected cells, Vpr is believed to function in the early phase of HIV-1 replication, including nuclear migration of preintegration complex, transcription of the provirus genome and viral multiplication by blocking cells in the G2 phase. Vpr has a critical role in long-term AIDS disease by inducing infection in nondividing cells such as monocytes and macrophages. Mutations have suggested that the N-terminal domain of Vpr encompassing the first 40 residues could be required for nuclear localization, packaging into virions and binding of transcription factor (TFIIB, Sp1), viral proteins (p6) and cellular proteins (RIP1, UNG, karyopherins). To gain insight into the structure-function relationship of Vpr, (1-51)Vpr was synthesized and its structure analyzed by circular dichroism and two-dimensional 1H NMR in aqueous trifluoroethanol (30%) solution and refined by restrained molecular dynamics. The structure is characterized by three turns around the first three prolines, Pro5, Pro10, Pro14, followed by a long amphipathic alpha helix-turn-alpha helix (Asp17-Ile46) motif ended by a turn extending from Tyr47 to Thr49. The alpha helix-turn-alpha helix motif and the amphipathic helix are well known for being implicated in protein-protein or protein-nucleic acid interaction. Therefore structural characteristics of the (1-51) N-terminal fragment of Vpr could explain why this region of Vpr plays a role in several biological functions of this protein.


Subject(s)
Gene Products, vpr/chemistry , Gene Products, vpr/genetics , Magnetic Resonance Spectroscopy/methods , Amino Acid Motifs , Amino Acid Sequence , Cell Nucleus/metabolism , Circular Dichroism , Humans , Lipid Metabolism , Models, Molecular , Molecular Sequence Data , Peptide Biosynthesis , Peptides/chemistry , Protein Structure, Secondary , Protein Structure, Tertiary , Temperature
4.
J Pept Res ; 54(5): 427-35, 1999 Nov.
Article in English | MEDLINE | ID: mdl-10563508

ABSTRACT

The 96-amino acid protein Vpr functions as a regulator of cellular processes involved in the human immunodeficiency virus, type 1 (HIV-1) life cycle, including cell-cycle arrest at the G2/M check point, promotion of the HIV-1 preintegration complex for nuclear transport, induction of apoptosis and transcriptional activation of a variety of viral and cellular promoters. Preliminary 1H NMR experiments performed on Vpr fragments showed the presence of several helical regions. However, the assignment of many protons in the amide region of the complete sequence of Vpr proved to be impossible due to the overlap of multiple NOE cross peaks. Moreover, because of its cytotoxicity, it is difficult to produce large quantities of 15N- and 13C-labeled Vpr using molecular biology approaches. Therefore, the solid-phase peptide synthesis of (1-96)Vpr, labeled at 22 selected positions, using recently commercially available uniformly 13C-, 15N-labeled fmoc amino acids, has been optimized to produce large quantities (104 mg, 15% yield) of pure compound, while minimizing the quantity of labeled amino acids used for each coupling. As expected two-dimensional heteronuclear NMR experiments performed with this protein allowed the unequivocal assignments of all the proton signals. This study shows that introduction of few labeled 13C/15N labeled amino acids in selected positions facilitates the determination of structure solution of small protein accessible by solid-phase peptide synthesis, and could allow dynamic studies of their conformational behavior to be carried out.


Subject(s)
Gene Products, vpr/chemical synthesis , HIV-1/chemistry , Amino Acid Sequence , Amino Acids/chemistry , Carbon Isotopes , Fluorenes/chemistry , Gene Products, vpr/chemistry , Magnetic Resonance Spectroscopy , Molecular Sequence Data , Nitrogen Isotopes , Peptide Fragments/chemistry , Protein Conformation , Protein Structure, Secondary , vpr Gene Products, Human Immunodeficiency Virus
5.
Biochemistry ; 38(40): 12984-94, 1999 Oct 05.
Article in English | MEDLINE | ID: mdl-10529168

ABSTRACT

The structure of the 56 amino acid nucleocapsid protein NCp10 of retrovirus MoMuLV, which contains a single CX(2)CX(4)HX(4)C-type zinc finger, has been determined previously by NMR. The important role of NCp10 (or NCp7 for HIV-1) in the retroviral life cycle seems mainly related to their preferential binding to single-stranded nucleic acids. We report here the structure of the complex formed between the biologically active (14-53)NCp10 and the oligonucleotide d(ACGCC) in aqueous solution determined by 2D (1)H NMR based methods. The aromatic residue Trp(35) of NCp10 directs nucleic acid complexation as shown by its complete fluorescence quenching upon addition of d(ACGCC). (1)H and (31)P NMR studies support the insertion of Trp(35) between the G(3) and C(4) bases. A total of 577 NOE distance restraints, of which 40 were intermolecular, were used for the structure determination. The zinc finger provides a well-defined surface for the binding of d(ACGCC) through hydrophobic interactions and tryptophan stacking on the guanine. This latter interaction was also observed in the NMR-derived structures of the complexes between NCp7, which contains two successive zinc fingers, and single-stranded DNA and RNA, supporting the proposal for a major role played by aromatic residues of NCp proteins in nucleic acid recognition. Upon binding to the nucleotide a new loop in NCp10 that participates in the intermolecular interaction is formed. Additional interactions provided by positively charged residues surrounding the zinc finger appear necessary for tight binding. The structure of the complex NCp10-d(ACGCC) gives a structural explanation for the loss of virus infectivity following point mutations in the finger domain.


Subject(s)
Capsid Proteins , Capsid/chemistry , Gene Products, gag/chemistry , HIV-1/chemistry , Moloney murine leukemia virus/chemistry , Oligonucleotides/chemistry , Viral Core Proteins/chemistry , Viral Proteins , Zinc Fingers , Amino Acid Sequence , Humans , Macromolecular Substances , Models, Molecular , Molecular Sequence Data , Nuclear Magnetic Resonance, Biomolecular/methods , Phosphorus Isotopes , Protein Conformation , Protons , Sequence Homology, Amino Acid , Spectrometry, Fluorescence , Structure-Activity Relationship , gag Gene Products, Human Immunodeficiency Virus
6.
J Mol Biol ; 285(5): 2105-17, 1999 Feb 05.
Article in English | MEDLINE | ID: mdl-9925788

ABSTRACT

The HIV-1 regulatory protein Vpr (96 amino acid residues) is incorporated into the virus particle through a mechanism involving its interaction with the C-terminal portion of Gag. Vpr potentiates virus replication by interrupting cell division in the G2 phase and participates in the nuclear transport of proviral DNA. The domain encompassing the 40 C-terminal residues of Vpr was shown to be involved in cell cycle arrest and binding of nucleocapsid protein NCp7, and suggested to promote nuclear provirus transfer. Accordingly, we show here that the synthetic 52-96 but not 1-51 sequences of Vpr interact with HIV-1 RNA. Based on these results, the structure of (52-96)Vpr was analysed by two-dimensional 1H-NMR in aqueous TFE (30%) solution and refined by restrained molecular dynamics. The structure is characterized by a long (53-78) amphipathic alpha-helix, followed by a less defined (79-96) C-terminal domain. The Leu60 and Leu67 side-chains are located on the hydrophobic side of the helix, suggesting their involvement in Vpr dimerization through a leucine zipper-type mechanism. Accordingly, their replacement by Ala eliminates Vpr dimerization in the two hybrid systems, while mutations of Ile74 and Ile81 have no effect. This was confirmed by gel filtration measurements and circular dichroism, which also showed that the alpha-helix still exists in (52-96)Vpr and its Ala60, Ala67 mutant in the presence and absence of TFE. Based on these results, a model of the coiled-coil Vpr dimer has been described, and its biological relevance as well as that of the structural characteristics of the 52-96 domain for the different functions of Vpr, including HIV-1 RNA binding, are discussed.


Subject(s)
Capsid Proteins , Gene Products, vpr/chemistry , Gene Products, vpr/metabolism , Viral Proteins , Amino Acid Sequence , Capsid/chemistry , Capsid/metabolism , Circular Dichroism , Dimerization , Gene Products, gag/chemistry , Gene Products, gag/metabolism , Gene Products, vpr/genetics , Magnetic Resonance Spectroscopy , Models, Molecular , Molecular Sequence Data , Nucleic Acids/chemistry , Nucleic Acids/metabolism , Peptide Fragments/chemistry , Peptide Fragments/metabolism , Protein Conformation , Protein Structure, Secondary , RNA, Viral/chemistry , RNA, Viral/metabolism , Recombinant Proteins/chemistry , Recombinant Proteins/genetics , Recombinant Proteins/metabolism , Yeasts/genetics , Zinc Fingers , gag Gene Products, Human Immunodeficiency Virus
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