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1.
Mycopathologia ; 186(1): 71-80, 2021 Mar.
Article in English | MEDLINE | ID: mdl-33216273

ABSTRACT

Dermatomycoses caused by Candida spp. are increasingly common, however there are few reports in the literature regarding their epidemiology, pathogenesis and antifungal susceptibility of these fungal pathogens. This study aimed to identify, characterize virulence and determine the antifungal susceptibility of Candida species isolated from patients with dermatomycoses. We studied a total of 45 yeast samples isolated from dermal scrapings from patients treated at a public hospital, Bauru, São Paulo, Brazil. After identification, the samples were analyzed for protease activity, phospholipase, biofilm formation and antifungal susceptibility. The most common species was Candida albicans (40%), followed by C. krusei (22.22%), C. parapsilosis complex (22.22%) and C. famata (6.67%). Regarding virulence mechanisms, 57.78% and 28.89% of the samples showed enzymatic activity for protease and phospholipase, respectively. A total of 13.33% of the samples showed secretion of both enzymes. All isolates were biofilm producers, and a higher production was observed in C. tropicalis isolates. Regarding the antifungal susceptibility test, 97.78% of the samples had the highest levels of the minimal inhibitory concentration (MIC) for terbinafine, and one C. krusei sample showed intermediate susceptibility to fluconazole. The correct identification of dermatomycosis-related microorganisms, as well as a better knowledge of their pathogenicity and susceptibility against the antifungals used in the clinic, is extremely important for an efficient preventive and therapeutic action.


Subject(s)
Antifungal Agents , Dermatomycoses , Antifungal Agents/pharmacology , Brazil , Candida/drug effects , Dermatomycoses/drug therapy , Drug Resistance, Fungal , Humans , Microbial Sensitivity Tests , Virulence Factors
2.
Rev. Salusvita (Online) ; 40(3): 166-172, 2021.
Article in Portuguese | LILACS | ID: biblio-1412375

ABSTRACT

O objetivo do presenteestudo foi avaliar a atividade antibacteriana dos cimentos endodôn-ticos MTA Fillapex, Sealapex, Sealer 26 e Endofill puros e acrescidos de amoxicilina e ci-profloxacina frente a duas linhagens ATCC de Enterococcus faecalis (ATCC 29212 e ATCC 4083). Os testes foram realizados pela técnica de difusão radial dos cimentos aplicados di-retamente no ágar e impregnados em discos de papel e pelo teste de contato direto. Para a análise estatística, utilizaram-se os testes de ANOVA e Tukey com nível de significância de 5%. Os resultados mostraram que pela técnica da difusão radial, considerando os cimentos puros, o Sealapex foi o único que apresentou halos de inibição frente às duas linhagens. Já quando da associação de amoxicilina e ciprofloxacina, o MTA Fillapex revelou os melhores halos de inibição para as duas linhagens. Todos os cimentos tiveram a ação potencializada pela adição dos antibióticos (p<0,05). Pela técnica de difusão dos cimentos impregnados em discos de papel, o cimento Sealapex puro fresco demonstrou atividade antimicrobiana em todas as variáveis de tempo. Quando acrescidos dos antibióticos, todos os cimentos ti-veram a ação antimicrobiana potencializada pelas drogas, em todas as variáveis de tempo. Esse mesmo resultado foi observado na técnica do contato direto dos cimentos com ambas as linhagens. A atividade antibacteriana, frente ao E. faecalis, dos cimentos MTA Fillapex, Endofill, Sealer e Sealapex, acrescidos dos antibióticos amoxicilina e ciprofloxacina, foi potencializada mesmo após um longo período da presa dos cimentos.


The objective of this study was to evaluate the antibacterial activity of different endodon-tic cements (MTA Fillapex, Sealapex, Sealer 26, and Endofill), both pure and mixed with 5% of amoxicillin or ciprofloxacin, against two strains of Enterococcus faecalis (ATCC 29212 and ATCC 4083). Bacterial susceptibility testing was conducted by radial diffusion methods and direct contact test after different periods. Data were subjected to ANO-VA and Tukey test with a significance level of 5%. Sealapex was the only pure sealer to promote the inhibition of bacterial growth against both strains in different experimental periods. However, all cements present an increase in antibacterial activity by the addition of antibiotics (p<0.05). MTA Fillapex and Sealapex associated with amoxicillin promoted increased values for bacterial growth against ATCC 29212 and ATCC 4083 in comparison to other groups (p<0.001), including extended periods after setting time. The association of antibiotic agents increased the antibacterial effect of the evaluated sealers against the strains of E. faecalis.


Subject(s)
Endodontics/instrumentation , Enterococcus faecalis
3.
Antibiotics (Basel) ; 9(12)2020 Dec 11.
Article in English | MEDLINE | ID: mdl-33322526

ABSTRACT

Dermatophyte fungal infections are difficult to treat because they need long-term treatments. 4-Nerolidylcatechol (4-NC) is a compound found in Piper umbellatum that has been reported to demonstrate significant antifungal activity, but is easily oxidizable. Due to this characteristic, the incorporation in nanostructured systems represents a strategy to guarantee the compound's stability compared to the isolated form and the possibility of improving antifungal activity. The objective of this study was to incorporate 4-NC into polymeric nanoparticles to evaluate, in vitro and in vivo, the growth inhibition of Microsporum canis. 4-NC was isolated from fresh leaves of P. umbellatum, and polymer nanoparticles of polycaprolactone were developed by nanoprecipitation using a 1:5 weight ratio (drug:polymer). Nanoparticles exhibited excellent encapsulation efficiency, and the antifungal activity was observed in nanoparticles with 4-NC incorporated. Polymeric nanoparticles can be a strategy employed for decreased cytotoxicity, increasing the stability and solubility of substances, as well as improving the efficacy of 4-NC.

4.
s.l; s.n; 2020. 16 p. ilus.
Non-conventional in English | Sec. Est. Saúde SP, CONASS, SESSP-ILSLPROD, Sec. Est. Saúde SP, SESSP-ILSLACERVO, Sec. Est. Saúde SP | ID: biblio-1146694

ABSTRACT

Dermatophyte fungal infections are difficult to treat because they need long-term treatments. 4-Nerolidylcatechol (4-NC) is a compound found in Piper umbellatum that has been reported to demonstrate significant antifungal activity, but is easily oxidizable. Due to this characteristic, the incorporation in nanostructured systems represents a strategy to guarantee the compound's stability compared to the isolated form and the possibility of improving antifungal activity. The objective of this study was to incorporate 4-NC into polymeric nanoparticles to evaluate, in vitro and in vivo, the growth inhibition of Microsporum canis. 4-NC was isolated from fresh leaves of P. Umbellatum, and polymer nanoparticles of polycaprolactone were developed by nanoprecipitation using a 1:5 weight ratio (drug:polymer). Nanoparticles exhibited excellent encapsulation efficiency, and the antifungal activity was observed in nanoparticles with 4-NC incorporated. Polymeric nanoparticles can be a strategy employed for decreased cytotoxicity, increasing the stability and solubility of substances, as well as improving the efficacy of 4-NC(AU).


Subject(s)
Nanoparticles/therapeutic use , Microsporum/growth & development , Antifungal Agents
5.
s.l; s.n; 2020. 10 p. tab.
Non-conventional in English | Sec. Est. Saúde SP, CONASS, SESSP-ILSLPROD, Sec. Est. Saúde SP, SESSP-ILSLACERVO, Sec. Est. Saúde SP | ID: biblio-1146782

ABSTRACT

Dermatomycoses caused by Candida spp. are increasingly common, however there are few reports in the literature regarding their epidemiology, pathogenesis and antifungal susceptibility of these fungal pathogens. This study aimed to identify, characterize virulence and determine the antifungal susceptibility of Candida species isolated from patients with dermatomycoses. We studied a total of 45 yeast samples isolated from dermal scrapings from patients treated at a public hospital, Bauru, São Paulo, Brazil. After identification, the samples were analyzed for protease activity, phospholipase, biofilm formation and antifungal susceptibility. The most common species was Candida albicans (40%), followed by C. krusei (22.22%), C. parapsilosis complex (22.22%) and C. famata (6.67%). Regarding virulence mechanisms, 57.78% and 28.89% of the samples showed enzymatic activity for protease and phospholipase, respectively. A total of 13.33% of the samples showed secretion of both enzymes. All isolates were biofilm producers, and a higher production was observed in C. tropicalis isolates. Regarding the antifungal susceptibility test, 97.78% of the samples had the highest levels of the minimal inhibitory concentration (MIC) for terbinafine, and one C. krusei sample showed intermediate susceptibility to fluconazole. The correct identification of dermatomycosis-related microorganisms, as well as a better knowledge of their pathogenicity and susceptibility against the antifungals used in the clinic, is extremely important for an efficient preventive and therapeutic action(AU).


Subject(s)
Humans , Candida/pathogenicity , Dermatomycoses/epidemiology , Virulence , Antifungal Agents/therapeutic use
6.
Dent. press endod ; 9(1): 43-47, jan.-mar. 2019. Ilus
Article in Portuguese | LILACS, BBO - Dentistry | ID: biblio-1008596

ABSTRACT

Introdução: a frequência de micoses invasivas causadas por patógenos fúngicos oportunistas tem aumentado significativamente nas últimas décadas. Entre os principais agentes etiológicos de micoses oportunistas está a Candida albicans. Essa levedura apresenta vários fatores de virulência importantes para produção de doença. Alguns estudos têm demonstrado que C. albicans coloniza canais radiculares e túbulos dentinários. O espectro de atividade antimicrobiana do curativo de hidróxido de cálcio deve incluir essa levedura. Objetivos: o objetivo da presente pesquisa foi determinar a suscetibilidade in vitro de quatro linhagens de C. albicans coletadas da cavidade oral e uma estirpe padrão ATCC 10231 a pastas de hidróxido de cálcio associadas a drogas antifúngicas, antibióticas e anti-inflamatórias. A eficiência das pastas de Ca(OH)2 associadas às drogas sobre as leveduras foram analisadas pelo método da difusão radial e, também, pela técnica do contato direto com os medicamentos. Os dados foram analisados pelo teste de Kruskal-Wallis e o pós-teste de Dunn, para indicar as diferenças entres os grupos, com um nível de significância de 5%. Resultados: todas as medicações antifúngicas potencializaram a ação das pastas de hidróxido de cálcio contra a Candida albicans. Conclusões: a associação de medicações antifúngicas às pastas de Ca(OH)2 pode ser uma alternativa como medicação intracanal. (AU)


Introduction: the frequency of invasive mycoses caused by opportunistic fungal pathogens has increased significantly in the last decades. Among the main etiological agents of opportunistic mycoses is Candida albicans. This yeast has several important virulence factors for producing disease. Some studies have demonstrated that C. albicans colonized root canals and dentinal tubules. The spectrum of antimicrobial activity of calcium hydroxide dressing should include this yeast. Objectives: the aim of the present study was to determine the in vitro susceptibility of four C. albicans strains collected from the oral cavity and a standard strain ATCC 10231 to calcium hydroxide paste associated with antifungal, antibiotic and anti-inflammatory drugs. The efficiency of the Ca(OH)2 paste associated with the drugs on the yeasts was analyzed by the radial diffusion method and also by the direct contact method. Data were analyzed by Kruskal Wallis test and the Dunn post-test were used to indicate the differences between the groups with a significance level of 5%. Results: all antifungal drugs increased the action of the calcium hydroxide pastes against Candida albicans. Conclusions: the association of antifungals with Ca(OH)2 may be considered for use as intracanal medicaments (AU).


Subject(s)
Calcium Hydroxide , Candida albicans , Endodontics , Root Canal Therapy
7.
J Endod ; 43(1): 131-134, 2017 Jan.
Article in English | MEDLINE | ID: mdl-27939738

ABSTRACT

INTRODUCTION: The objective of the present study was to evaluate the in vitro antibiofilm activity and pH of calcium hydroxide associated with different nonsteroidal anti-inflammatory drugs (NSAIDs). METHODS: The groups analyzed were as follows: group 1, calcium hydroxide paste with propylene glycol; group 2, calcium hydroxide paste with propylene glycol + 5% diclofenac sodium; group 3, calcium hydroxide paste with propylene glycol + 5% ibuprofen; group 4, calcium hydroxide paste with propylene glycol + 5% ciprofloxacin; and group 6, positive control (without medication). For analysis of the pH, the pastes were inserted into tubes and immersed in flasks containing ultrapure water. At the time intervals of 3, 24, 72, and 168 hours, the pH was measured with a calibrated pH meter. For microbial analysis, biofilm was induced in 30 bovine dentin blocks for 21 days. Subsequently, the pastes were placed on the blocks with biofilm for 7 days. Afterward, the pastes were removed by irrigation with sterile water, and the specimens were analyzed with a laser scanning confocal microscope with the 50 µL Live/Dead BacLight Bacterial Viability solution L7012 Kit (Molecular Probes, Inc, Eugene, OR). Data were subjected to statistical analysis at a significance level of 5%. RESULTS: The highest pH values were found for calcium hydroxide associated with ciprofloxacin in all periods analyzed. With the exception of pure calcium hydroxide paste, the other groups showed statistically significant differences (P < .05) in comparison with the positive control. CONCLUSIONS: The association of NSAIDs or antibiotic did not interfere with the pH of calcium hydroxide paste and increased the antimicrobial action of calcium hydroxide paste against Enterococcus faecalis biofilm formation.


Subject(s)
Anti-Bacterial Agents/therapeutic use , Anti-Inflammatory Agents, Non-Steroidal/therapeutic use , Biofilms/drug effects , Calcium Hydroxide/therapeutic use , Dental Cements/therapeutic use , Enterococcus faecalis/drug effects , Anti-Bacterial Agents/administration & dosage , Anti-Inflammatory Agents, Non-Steroidal/administration & dosage , Diclofenac/administration & dosage , Diclofenac/therapeutic use , Drug Therapy, Combination , Enterococcus faecalis/growth & development , Hydrogen-Ion Concentration/drug effects , In Vitro Techniques
8.
Cleft Palate Craniofac J ; 51(2): 145-53, 2014 Mar.
Article in English | MEDLINE | ID: mdl-22594401

ABSTRACT

OBJECTIVE: To characterize the microbial etiology of chronic suppurative otitis media comparing the methods of classical bacteriological culture and polymerase chain reaction. DESIGN/SETTING/PATIENTS: Bacteriological analysis by classical culture and by molecular polymerase chain reaction of 35 effusion otitis samples from patients with cleft lip and palate attending the Hospital for Rehabilitation of Craniofacial Anomalies of the University of São Paulo, Bauru, Brazil. INTERVENTIONS: Collection of clinical samples of otitis by effusion through the external auditory tube. MAIN OUTCOME MEASURE: Otolaryngologic diagnosis of chronic suppurative otitis media. RESULTS: Positive cultures were obtained from 83% of patients. Among the 31 bacterial lineages the following were isolated. In order of decreasing frequency: Pseudomonas aeruginosa (54.9%), Staphylococcus aureus (25.9%), and Enterococcus faecalis (19.2%). No anaerobes were isolated by culture. The polymerase chain reaction was positive for one or more bacteria investigated in 97.1% of samples. Anaerobe lineages were detected by the polymerase chain reaction method, such as Fusobacterium nucleatum , Bacteroides fragilis , and Peptostreptococcus anaerobius . CONCLUSIONS: Patients with cleft lip and palate with chronic suppurative otitis media presented high frequency of bacterial infection in the middle ear. The classical bacteriological culture did not detect strict anaerobes, whose presence was identified by the polymerase chain reaction method.


Subject(s)
Bacterial Infections/microbiology , Cleft Lip/complications , Cleft Palate/complications , Otitis Media, Suppurative/microbiology , Chronic Disease , DNA, Bacterial/analysis , Female , Humans , Male , Polymerase Chain Reaction
9.
Bauru; s.n; 2014. 26 p. ilus.
Non-conventional in Portuguese | Sec. Est. Saúde SP, SESSP-ILSLPROD, Sec. Est. Saúde SP, SESSP-PAPSESSP, Sec. Est. Saúde SP | ID: biblio-1083599

ABSTRACT

A dermatofitose é uma doença fúngica, contagiosa, causada por um grupo de fungos chamados dermatófitos que tem uma forte afinidade por tecidos queratinizados da pele e seus apêndices (pêlo e unha). O uso de antifúngicos à longo prazo; de forma indiscriminada; associados a doses relativamente elevadas, pode selecionar micro-organismos resistentes e provocar efeitos colaterais severos, faz com que busquemos saídas para amenizar este quadro. Diante do exposto, avaliamos se os fitoterápicos em estudo ( óleo de Cravo-da-Índia, óleo de Melaleuca, óleo de Eucalipto e óleo de Citronela) possuem efeitos inibidores no controle e tratamento dos dermatófitos mais frequentes.OBJETIVOS: Avaliar se os óleos essenciais de Cravo-da-India, Melaleuca, Eucalipto e Citronela possuem efeitos inibitórios no controle e tratamento dos dermatófitos estudados, buscando alternativas naturais. MATERIAL E MÉTODOS: Foram utilizadas cepas de linhagens de dermatófitos Trichophyton rubrum e Trichophyton mentagrophytes isolados da rotina laboratorial do Laboratório de Micologia do Instituto Lauro de Souza Lima/Bauru, por demanda espontânea, de diferentes sítios anatômicos, sendo estes inoculados em placas de Petri contendo óleos de: cravo-da-India, melaleuca, eucalipto e citronela em 5 concentrações diferentes. Estas placas foram incubadas em estufa bacteriológica a 25°C durante 7 dias. Foram feitos controles positivos e controles negativos para cada amostra e também realizadas o mesmo procedimento com 4 cepas padrão (American Type Culture Collection) das mesmas linhagens de dermatófitos. Após o sétimo dia de inoculação, as leituras do diâmetro das colônias foram feitas e os resultados foram avaliados quanto ao óleo, ao agente etiológico, ao sexo e ao sítio anatômico. RESULTADOS E DISCUSSÃO: Os resultados mostraram a inibição dos microrganismos nas amostras em 99% do óleo de Cravo-da-índia, em 84% na Citronela, em 40% na Melaleuca e 15% no Eucalipto independente da e, do sítio ...


Subject(s)
Antifungal Agents , Phytotherapy , Phytotherapeutic Drugs , Tinea/drug therapy , Cymbopogon , Eucalyptus , Eugenia , Plant Preparations/therapeutic use , Trichophyton/isolation & purification , In Vitro Techniques , Tea Tree Oil/therapeutic use
10.
Braz Dent J ; 23(3): 192-8, 2012.
Article in English | MEDLINE | ID: mdl-22814685

ABSTRACT

Candida albicans is present in the oral cavity and in the whole digestive tract of humans and other animals, being frequently related to endodontic treatment failure. The present study determined the incidence of C. albicans in the oral cavity and the susceptibility of isolates to different pH values and saturated calcium hydroxide aqueous solution at pH 12.5. Sixty-five patients attending the Endodontic Clinic at the Sagrado Coração University participated in the study. The collected samples were cultivated in selective media for C. albicans and the isolates were tested in terms of resistance to both alkaline pH and saturated aqueous solution of calcium hydroxide. In relation to time variables, yeast viability was assessed by the Sabouraud's agar culture and fluorescein diacetate and ethidium bromide fluorescent staining method. Results from the different pHs and experimental times, including those from different techniques measuring fungal viability, were compared using the chi-square and Fisher's exact tests (α=0.05). The yeasts became completely inviable after 48 h of contact with the calcium hydroxide solution. On the other hand, when exposed to the alkaline culture broth, the yeasts were found to be viable at pHs 9.5 and 10.5 for up to 7 days. In conclusion, C. albicans can only be completely inhibited by direct contact with saturated calcium hydroxide aqueous solution after 48 h of exposure.


Subject(s)
Calcium Hydroxide/pharmacology , Candida albicans/drug effects , Mouth/microbiology , Root Canal Irrigants/pharmacology , Yeasts/drug effects , Candida albicans/isolation & purification , Humans , Hydrogen-Ion Concentration , Microbial Sensitivity Tests , Mouth/drug effects , Staining and Labeling
11.
s.l; s.n; 2012. 7 p. graf.
Non-conventional in English | Sec. Est. Saúde SP, SESSP-ILSLPROD, Sec. Est. Saúde SP, SESSP-ILSLACERVO, Sec. Est. Saúde SP | ID: biblio-1096110

ABSTRACT

Candida albicans is present in the oral cavity and in the whole digestive tract of humans and other animals, being frequently related to endodontic treatment failure. The present study determined the incidence of C. albicans in the oral cavity and the susceptibility of isolates to different pH values and saturated calcium hydroxide aqueous solution at pH 12.5. Sixty-five patients attending the Endodontic Clinic at the Sagrado Coração University participated in the study. The collected samples were cultivated in selective media for C. albicans and the isolates were tested in terms of resistance to both alkaline pH and saturated aqueous solution of calcium hydroxide. In relation to time variables, yeast viability was assessed by the Sabouraud's agar culture and fluorescein diacetate and ethidium bromide fluorescent staining method. Results from the different pHs and experimental times, including those from different techniques measuring fungal viability, were compared using the chi-square and Fisher's exact tests (α=0.05). The yeasts became completely inviable after 48 h of contact with the calcium hydroxide solution. On the other hand, when exposed to the alkaline culture broth, the yeasts were found to be viable at pHs 9.5 and 10.5 for up to 7 days. In conclusion, C. albicans can only be completely inhibited by direct contact with saturated calcium hydroxide aqueous solution after 48 h of exposure.


Candida albicans está presente na cavidade oral e em todo trato digestivo de humanos e outros animais, podendo estar relacionada a fracassos do tratamento endodôntico. O presente trabalho determinou a incidência de C. albicans na cavidade oral e a susceptibilidade das amostras isoladas frente a diferentes valores de pH e à solução aquosa saturada de hidróxido de cálcio em pH 12,5. Sessenta e cinco pacientes atendidos na Clínica de Endodontia da Universidade Sagrado Coração participaram da pesquisa. As amostras coletadas foram cultivadas em meios seletivos para C. albicans. As cepas isoladas foram testadas quanto a resistência ao pH alcalino e à solução saturada de hidróxido de cálcio. Frente às variáveis de tempo, a viabilidade das leveduras foi avaliada pelos métodos da cultura sobre ágar Sabouraud e de fluorescência pela técnica do diacetato de fluoresceína e brometo de etídio. Foram isoladas 30 linhagens de C. albicans coletadas da cavidade oral dos pacientes. Quando em contato com a solução de hidróxido de cálcio as leveduras foram totalmente inviabilizadas após 48 h de exposição. Quando expostas ao caldo de cultura alcalinizado as leveduras mantiveram-se viáveis em pH 9,5 e 10,5 por até 7 dias. Em conclusão, C. albicans pode ser completamente inibida pelo contato direto com solução aquosa saturada de hidróxido de cálcio após 48 h de exposição.


Subject(s)
Humans , Root Canal Irrigants/pharmacology , Staining and Labeling , Yeasts/drug effects , Calcium Hydroxide/pharmacology , Candida albicans/isolation & purification , Candida albicans/drug effects , Hydrogen-Ion Concentration , Mouth/drug effects , Mouth/microbiology
12.
Braz. dent. j ; 23(3): 192-198, 2012. ilus
Article in English | LILACS | ID: lil-641586

ABSTRACT

Candida albicans is present in the oral cavity and in the whole digestive tract of humans and other animals, being frequently related to endodontic treatment failure. The present study determined the incidence of C. albicans in the oral cavity and the susceptibility of isolates to different pH values and saturated calcium hydroxide aqueous solution at pH 12.5. Sixty-five patients attending the Endodontic Clinic at the Sagrado Coração University participated in the study. The collected samples were cultivated in selective media for C. albicans and the isolates were tested in terms of resistance to both alkaline pH and saturated aqueous solution of calcium hydroxide. In relation to time variables, yeast viability was assessed by the Sabouraud's agar culture and fluorescein diacetate and ethidium bromide fluorescent staining method. Results from the different pHs and experimental times, including those from different techniques measuring fungal viability, were compared using the chi-square and Fisher's exact tests (α=0.05). The yeasts became completely inviable after 48 h of contact with the calcium hydroxide solution. On the other hand, when exposed to the alkaline culture broth, the yeasts were found to be viable at pHs 9.5 and 10.5 for up to 7 days. In conclusion, C. albicans can only be completely inhibited by direct contact with saturated calcium hydroxide aqueous solution after 48 h of exposure.


Candida albicans está presente na cavidade oral e em todo trato digestivo de humanos e outros animais, podendo estar relacionada a fracassos do tratamento endodôntico. O presente trabalho determinou a incidência de C. albicans na cavidade oral e a susceptibilidade das amostras isoladas frente a diferentes valores de pH e à solução aquosa saturada de hidróxido de cálcio em pH 12,5. Sessenta e cinco pacientes atendidos na Clínica de Endodontia da Universidade Sagrado Coração participaram da pesquisa. As amostras coletadas foram cultivadas em meios seletivos para C. albicans. As cepas isoladas foram testadas quanto a resistência ao pH alcalino e à solução saturada de hidróxido de cálcio. Frente às variáveis de tempo, a viabilidade das leveduras foi avaliada pelos métodos da cultura sobre ágar Sabouraud e de fluorescência pela técnica do diacetato de fluoresceína e brometo de etídio. Foram isoladas 30 linhagens de C. albicans coletadas da cavidade oral dos pacientes. Quando em contato com a solução de hidróxido de cálcio as leveduras foram totalmente inviabilizadas após 48 h de exposição. Quando expostas ao caldo de cultura alcalinizado as leveduras mantiveram-se viáveis em pH 9,5 e 10,5 por até 7 dias. Em conclusão, C. albicans pode ser completamente inibida pelo contato direto com solução aquosa saturada de hidróxido de cálcio após 48 h de exposição.


Subject(s)
Humans , Calcium Hydroxide/pharmacology , Candida albicans/drug effects , Mouth/microbiology , Root Canal Irrigants/pharmacology , Yeasts/drug effects , Candida albicans/isolation & purification , Hydrogen-Ion Concentration , Microbial Sensitivity Tests , Mouth/drug effects , Staining and Labeling
13.
Dent. press endod ; 1(1): 46-51, 2011. tab
Article in English, Portuguese | LILACS, Sec. Est. Saúde SP | ID: lil-685762

ABSTRACT

Objetivo: determinar o efeito antimicrobiano em contato direto de Casearia sylvestris Swart (guaçatonga), propilenoglicol,e da clorexidina associada ao hidróxido de cálcio contra 40 cepas de Enterococcus faecalis isoladas da cavidade bucal. Métodos: após a ativação, as cepas bacterianas foram suspensas em solução salina estéril até a escala 1,0 de MacFarland. A suspensão foi colocada em contato direto com hidróxido de cálcio [Ca(OH)2] + propilenoglicol puro, Ca(OH)2 + clorexidina a 1% em propilenoglicol, e Ca(OH)2 + extrato de guaçatonga em propilenoglicol, cobrindo pontas de papel, previamente contaminadas com a solução por 3 minutos, com as diferentes pastas. A atividade antimicrobiana foi avaliada em 6, 24, 48, 72 horas, e aos 7 dias. Após o período de incubação, os cones foram retirados das pastas e incubados em caldo Letheen a 37ºC por 48 horas. Em seguida, 0,1 ml do caldo Letheen foi transferido para tubos contendo infusão de cérebro e coração(BHI) e incubados em caldo novamente a 37ºC por 48 horas.A turbidez foi observada no meio. Depois disso, placas de ágar M-Enterococcus foram semeadas com caldo BHI de cada tubo e o crescimento colonial foi avaliado. Resultados: todas as cepas bacterianas foram inibidas por todas as pastas, nos períodos avaliados. Conclusões: concluiu-se que a adição dessas substâncias ao hidróxido de cálcio não interferiu no seu efeito antimicrobiano direto.


Objective: To determinate the direct antimicrobial effectsof Casearia sylvestris Swart (guaçatonga), propylene glycol,and of chlorhexidine associated to calcium hydroxidepaste against 40 Enterococcus faecalis strains isolated fromthe oral cavity when direct contact. Methods: After activation,the bacterial strains were suspended in sterile salineto 1.0 McFarland standard. The suspension was placedin direct contact with calcium hydroxide paste [Ca(OH)2]+ pure propylene glycol, Ca(OH)2 + chlorhexidine 1%in propylene glycol, and Ca(OH)2 + guaçatonga extractin propylene glycol by covering paper points, previouslycontaminated for 3 minutes, with the different pastes. Antimicrobialactivity was evaluated at 6, 24, 48, 72 hours,and at 7 days. After the incubation period, the points wereremoved from the pastes and incubated in Letheen brothat 37oC for 48 hours. Following that, 0.1ml of the Letheenbroth was transferred to tubes containing brain heart infusion(BHI) broth and incubated again at 37oC for 48hours. Turbidity was observed in the medium. After that,M-Enterococcus agar plates were seeded with BHI brothfrom each tube and colony growth was assessed. Results:All the bacterial strains were inhibited by all pastes at theevaluated periods. Conclusions: It was concluded that theaddition of these substances to calcium hydroxide did notinterfere with its direct antimicrobial effect.


Subject(s)
Casearia , Enterococcus faecalis , Calcium Hydroxide , Microbiology
14.
In. Jornada Dermatológica Paulista (141. : 2011 : Bauru); Sociedade Brasileira de Dermatologia. 141ª Jornada Dermatológica Paulista. Bauru, Instituto Lauro de Souza Lima, 2011. p.12-12.
Monography in Portuguese | Sec. Est. Saúde SP, SESSP-ILSLPROD, Sec. Est. Saúde SP, SESSP-ILSLACERVO, Sec. Est. Saúde SP | ID: biblio-1086800
15.
Cleft Palate Craniofac J ; 46(5): 461-7, 2009 Sep.
Article in English | MEDLINE | ID: mdl-19929097

ABSTRACT

OBJECTIVE: To investigate the microbial etiology of suppurative chronic otitis media (SCOM) in patients with complete cleft lip and palate and isolated cleft palate and to determine the sensitivity of isolated microorganisms to antibiotics by drug diffusion from impregnated discs in agar and the minimum inhibitory concentration of each drug to these microorganisms by drug dilution in agar. DESIGN/PATIENTS: Effusion samples of SCOM obtained from 40 patients with cleft lip and palate registered at the Hospital for Rehabilitation of Craniofacial Anomalies, University of São Paulo, at Bauru, Brazil, were bacteriologically analyzed by cultures. The isolated bacteria were submitted to an in vitro susceptibility test to clinically used drugs. RESULTS: Positive cultures were obtained in 100% of studied cases. Among the 57 strains observed, the most frequent were Pseudomonas aeruginosa (35%), Staphylococcus aureus (15.5%), Enterococcus faecalis (14%), and Proteus mirabilis (12%). The frequency of Gram-negative bacilli (enterobacteriaceae and nonfermentative bacilli) was 67%. Pseudomonas aeruginosa presented the highest sensitivity to ciprofloxacin, and enterobacteriaceae exhibited the highest sensitivity to gentamicin. The strains of S. aureus and E. faecalis presented the highest sensitivity to imipenem and sulfamethoxazole/trimethoprim, respectively. CONCLUSION: Patients with cleft lip and palate presenting with SCOM exhibited 100% positive cultures, with the highest frequency of Pseudomonas and enterobacteriaceae. With regard to the action of antibiotics, imipenem was effective against the four species of isolated microorganisms, followed by ciprofloxacin, which was effective against 75% of isolated species.


Subject(s)
Bacteria/classification , Cleft Palate/complications , Drug Resistance, Bacterial , Otitis Media, Suppurative/microbiology , Adolescent , Adult , Anti-Bacterial Agents/pharmacology , Child , Child, Preschool , Chronic Disease , Ciprofloxacin/pharmacology , Cleft Lip/complications , Enterococcus faecalis/drug effects , Female , Gentamicins/pharmacology , Gram-Positive Bacterial Infections/diagnosis , Humans , Imipenem/pharmacology , Infant , Male , Middle Aged , Proteus Infections/diagnosis , Proteus mirabilis/drug effects , Pseudomonas Infections/diagnosis , Pseudomonas aeruginosa/drug effects , Staphylococcal Infections/diagnosis , Staphylococcus aureus/drug effects , Trimethoprim, Sulfamethoxazole Drug Combination/pharmacology , Young Adult
16.
s.l; s.n; 2009. 7 p. tab.
Non-conventional in English | Sec. Est. Saúde SP, SESSP-ILSLPROD, Sec. Est. Saúde SP, SESSP-ILSLACERVO, Sec. Est. Saúde SP | ID: biblio-1096358

ABSTRACT

OBJECTIVE: To investigate the microbial etiology of suppurative chronic otitis media (SCOM) in patients with complete cleft lip and palate and isolated cleft palate and to determine the sensitivity of isolated microorganisms to antibiotics by drug diffusion from impregnated discs in agar and the minimum inhibitory concentration of each drug to these microorganisms by drug dilution in agar. DESIGN/PATIENTS Effusion samples of SCOM obtained from 40 patients with cleft lip and palate registered at the Hospital for Rehabilitation of Craniofacial Anomalies, University of São Paulo, at Bauru, Brazil, were bacteriologically analyzed by cultures. The isolated bacteria were submitted to an in vitro susceptibility test to clinically used drugs. RESULTS: Positive cultures were obtained in 100% of studied cases. Among the 57 strains observed, the most frequent were Pseudomonas aeruginosa (35%), Staphylococcus aureus (15.5%), Enterococcus faecalis (14%), and Proteus mirabilis (12%). The frequency of Gram-negative bacilli (enterobacteriaceae and nonfermentative bacilli) was 67%. Pseudomonas aeruginosa presented the highest sensitivity to ciprofloxacin, and enterobacteriaceae exhibited the highest sensitivity to gentamicin. The strains of S. aureus and E. faecalis presented the highest sensitivity to imipenem and sulfamethoxazole/trimethoprim, respectively. CONCLUSION: Patients with cleft lip and palate presenting with SCOM exhibited 100% positive cultures, with the highest frequency of Pseudomonas and enterobacteriaceae. With regard to the action of antibiotics, imipenem was effective against the four species of isolated microorganisms, followed by ciprofloxacin, which was effective against 75% of isolated species.


Subject(s)
Humans , Male , Infant , Child, Preschool , Child , Middle Aged , Young Adult , Otitis Media, Suppurative/microbiology , Proteus mirabilis/drug effects , Pseudomonas Infections/diagnosis , Bacteria/classification , Gentamicins/pharmacology , Ciprofloxacin/pharmacology , Chronic Disease , Cleft Lip/complications , Cleft Palate/complications , Gram-Positive Bacterial Infections/diagnosis , Enterococcus faecalis/drug effects , Drug Resistance, Bacterial , Anti-Bacterial Agents/pharmacology
17.
Rev. Salusvita (Online) ; 27(2): 119-134, 2008.
Article in Portuguese | LILACS, Sec. Est. Saúde SP, SESSP-ILSLPROD, Sec. Est. Saúde SP, SESSP-ILSLACERVO, Sec. Est. Saúde SP | ID: lil-562306

ABSTRACT

Enterococcus faecalis são cocos Gram positivos, elipsóides, em cadeias curtas, anaeróbios facultativos, habitantes dos tratos intestinal e genital e da cavidade oral de humanos e animais. Podem causar uma gama muito grande de doenças no homem como cistite, endocardite e infecções de feridas. Os enterococos têm sido implicados também em infecções endodônticas, comumente detectados em canais radiculares obturados exibindo sinais de periodontite apical crônica e lesões refratárias periapicais. É de preocupação do cirurgião dentista um completo saneamento do sistema de canais radiculares pela aplicação de medicação antisséptica entre sessões. Diante desta problemática, este estudo comparou a atividade antimicrobiana de extratos alcoólicos e aquoso da Casearia sylvestris Sw (Guaçatonga) com a atividade antimicrobiana da amoxicilina, azitromicina, vancomicina, penicilina, clindamicina, cloranfenicol e da clorexidina frente a 50 linhagens de E. faecalis isolados da cavidade oral de pacientes da Clínica de Endodontia da USC. Os testes foram realizadospelo método de difusão das drogas a partir de discos impregnados com as substâncias sobre a superfície de placas de ágar Mueller-Hinton. A droga de melhor eficiência foi amoxicilina com 100% das linhagens sensíveis, estatisticamente signifi cativo (p<0,05) quando comparadas às outras drogas. A droga de menor eficiência foi a clindamicina com 94% de linhagens resistentes. Dos extratos obtidos daplanta, o extrato propilenoglicólico mostrou melhor efi ciência, inibindo34 (68%) das estirpes (média de 4,31mm de halo de inibição). A clorexidina inibiu 100% das estirpes (média de 17,09mm de halo de inibição) (CNPq).


Subject(s)
Humans , Anti-Bacterial Agents/therapeutic use , Casearia , Enterococcus faecalis , Chlorhexidine , Endodontics
19.
JBC j. bras. clin. odontol. integr ; 6(32): 155-157, mar.-abr. 2002. tab
Article in Portuguese | LILACS, BBO - Dentistry | ID: lil-345081

ABSTRACT

A contaminaçäo bacteriana e fúngica presente no MTA-Angelus (cinza e branco) e do cimento Portland de um saco recém-aberto e de outro aberto há 2 meses foi avaliada. Os materiais foram colocados em 3 mL de caldo BHI ágar e incubados a 37ºC por 24 horas e em 3mL de caldo Sabouraud e incubados a 25ºC por 72 horas. Posteriormente, foi realizada a agitaçäo e repique dos caldos em placas com meios específicos para o crescimento de Gram+, Gram-, Staphylococcus, Pseudomonas, Enterococcus e fungos. As placas com meio específico para bactérias foram incubadas a 37ºC por 24 horas e as com meio específico para fungos mantidas a 25ºC por 15 dias. Os resultados mostraram näo haver contaminaçäo nos materiais testados


Subject(s)
Biological Contamination , Dental Cements , Oxides , Bacteria , Fungi
20.
Bauru; s.n; 2000. 14 p. tab, 30cm.
Non-conventional in Portuguese | Sec. Est. Saúde SP, SESSP-ILSLPROD, Sec. Est. Saúde SP, SESSP-ILSLACERVO, Sec. Est. Saúde SP, SESSP-PAPSESSP, Sec. Est. Saúde SP | ID: biblio-1083764
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