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2.
Clin Exp Immunol ; 187(2): 304-315, 2017 Feb.
Article in English | MEDLINE | ID: mdl-27784126

ABSTRACT

Various complement-mediated renal disorders are treated currently with the complement inhibitor eculizumab. By blocking the cleavage of C5, this monoclonal antibody prevents cell damage caused by complement-mediated inflammation. We included 23 patients with atypical haemolytic uraemic syndrome (aHUS, n = 12), C3 glomerulopathies (C3G, n = 9) and acute antibody-mediated renal graft rejection (AMR, n = 2), treated with eculizumab in 12 hospitals in Germany. We explored the course of complement activation biomarkers and the benefit of therapeutic drug monitoring of eculizumab. Complement activation was assessed by analysing the haemolytic complement function of the classical (CH50) and the alternative pathway (APH50), C3 and the activation products C3d, C5a and sC5b-9 prior to, 3 and 6 months after eculizumab treatment. Eculizumab concentrations were determined by a newly established specific enzyme-linked immunosorbent assay (ELISA). Serum eculizumab concentrations up to 1082 µg/ml point to drug accumulation, especially in paediatric patients. Loss of the therapeutic antibody via urine with concentrations up to 56 µg/ml correlated with proteinuria. In aHUS patients, effective complement inhibition was demonstrated by significant reductions of CH50, APH50, C3d and sC5b-9 levels, whereas C5a levels were only reduced significantly after 6 months' treatment. C3G patients presented increased C3d and consistently low C3 levels, reflecting ongoing complement activation and consumption at the C3 level, despite eculizumab treatment. A comprehensive complement analysis together with drug monitoring is required to distinguish mode of complement activation and efficacy of eculizumab treatment in distinct renal disorders. Accumulation of the anti-C5 antibody points to the need for a patient-orientated tailored therapy.


Subject(s)
Antibodies, Monoclonal, Humanized/therapeutic use , Atypical Hemolytic Uremic Syndrome/drug therapy , Complement C3/immunology , Glomerulonephritis, Membranous/drug therapy , Graft Rejection/prevention & control , Immunosuppressive Agents/therapeutic use , Kidney Transplantation , Adolescent , Adult , Antibody-Dependent Cell Cytotoxicity/drug effects , Biomarkers/metabolism , Child , Child, Preschool , Complement Activation/drug effects , Complement C5/immunology , Female , Humans , Infant , Male , Middle Aged , Young Adult
3.
Eur Cell Mater ; 30: 118-30; discussion 130-1, 2015 Sep 21.
Article in English | MEDLINE | ID: mdl-26388615

ABSTRACT

Large segmental defects in bone fail to heal and remain a clinical problem. Muscle is highly osteogenic, and preliminary data suggest that autologous muscle tissue expressing bone morphogenetic protein-2 (BMP-2) efficiently heals critical size defects in rats. Translation into possible human clinical trials requires, inter alia, demonstration of efficacy in a large animal, such as the sheep. Scale-up is fraught with numerous biological, anatomical, mechanical and structural variables, which cannot be addressed systematically because of cost and other practical issues. For this reason, we developed a translational model enabling us to isolate the biological question of whether sheep muscle, transduced with adenovirus expressing BMP-2, could heal critical size defects in vivo. Initial experiments in athymic rats noted strong healing in only about one-third of animals because of unexpected immune responses to sheep antigens. For this reason, subsequent experiments were performed with Fischer rats under transient immunosuppression. Such experiments confirmed remarkably rapid and reliable healing of the defects in all rats, with bridging by 2 weeks and remodelling as early as 3-4 weeks, despite BMP-2 production only in nanogram quantities and persisting for only 1-3 weeks. By 8 weeks the healed defects contained well-organised new bone with advanced neo-cortication and abundant marrow. Bone mineral content and mechanical strength were close to normal values. These data demonstrate the utility of this model when adapting this technology for bone healing in sheep, as a prelude to human clinical trials.


Subject(s)
Bone Morphogenetic Protein 2/metabolism , Bone Regeneration/genetics , Bone and Bones/injuries , Bone and Bones/metabolism , Fracture Healing/genetics , Muscle, Skeletal/metabolism , Animals , Animals, Genetically Modified , Bone Morphogenetic Protein 2/genetics , Genetic Therapy , Genetic Vectors/therapeutic use , Male , Rats , Sheep , Transforming Growth Factor beta/genetics
6.
Am J Physiol Lung Cell Mol Physiol ; 289(6): L1083-93, 2005 Dec.
Article in English | MEDLINE | ID: mdl-16085670

ABSTRACT

Loss of PKC-epsilon limits the magnitude of acute hypoxic pulmonary vasoconstriction (HPV) in the mouse. Therefore, we hypothesized that loss of PKC-epsilon would decrease the contractile and/or structural response of the murine pulmonary circulation to chronic hypoxia (Hx). However, the pattern of lung vascular responses to chronic Hx may or may not be predicted by the acute HPV response. Adult PKC-epsilon wild-type (PKC-epsilon(+/+)), heterozygous null, and homozygous null (PKC-epsilon(-/-)) mice were exposed to normoxia or Hx for 5 wk. PKC-epsilon(-/-) mice actually had a greater increase in right ventricular (RV) systolic pressure, RV mass, and hematocrit in response to chronic Hx than PKC-epsilon(+/+) mice. In contrast to the augmented PA pressure and RV hypertrophy, pulmonary vascular remodeling was increased less than expected (i.e., equal to PKC-epsilon(+/+) mice) in both the proximal and distal PKC-epsilon(-/-) pulmonary vasculature. The contribution of increased vascular tone to this pulmonary hypertension (PHTN) was assessed by measuring the acute vasodilator response to nitric oxide (NO). Acute inhalation of NO reversed the increased PA pressure in hypoxic PKC-epsilon(-/-) mice, implying that the exaggerated PHTN may be due to a relative deficiency in nitric oxide synthase (NOS). Despite the higher PA pressure, chronic Hx stimulated less of an increase in lung endothelial (e) and inducible (i) NOS expression in PKC-epsilon(-/-) than PKC-epsilon(+/+) mice. In contrast, expression of nNOS in PKC-epsilon(+/+) mice decreased in response to chronic Hx, while lung levels in PKC-epsilon(-/-) mice remained unchanged. In summary, loss of PKC-epsilon results in increased vascular tone, but not pulmonary vascular remodeling in response to chronic Hx. Blunting of Hx-induced eNOS and iNOS expression may contribute to the increased vascular tone. PKC-epsilon appears to be an important signaling intermediate in the hypoxic regulation of each NOS isoform.


Subject(s)
Hypoxia/enzymology , Lung/blood supply , Lung/enzymology , Protein Kinase C-epsilon/metabolism , Pulmonary Circulation , Animals , Blood Pressure/drug effects , Blood Pressure/genetics , Female , Gene Expression Regulation, Enzymologic/drug effects , Gene Expression Regulation, Enzymologic/genetics , Hypertension, Pulmonary/enzymology , Hypertension, Pulmonary/genetics , Hypertension, Pulmonary/pathology , Hypoxia/genetics , Hypoxia/pathology , Lung/pathology , Male , Mice , Mice, Knockout , Nitric Oxide/administration & dosage , Nitric Oxide/metabolism , Nitric Oxide Synthase Type II/biosynthesis , Nitric Oxide Synthase Type III , Protein Kinase C-epsilon/deficiency , Protein Kinase C-epsilon/genetics , Pulmonary Circulation/drug effects , Pulmonary Circulation/genetics , Signal Transduction/drug effects , Signal Transduction/genetics
7.
Hosp Community Psychiatry ; 38(9): 988-91, 1987 Sep.
Article in English | MEDLINE | ID: mdl-3679106

ABSTRACT

Art therapy has lagged behind other therapeutic modalities in being subjected to rigorous evaluation of its effectiveness. This study examines psychosocial outcome for a group of chronic psychiatric outpatients. Half the patients were randomly assigned to a ten-week supportive art therapy group as an adjunct to treatment; the other patients served as a control group. Patients who remained in the art therapy group for the full ten weeks reported a significant improvement in their attitudes toward themselves as measured by the Progress Evaluation Scales, and their therapists rated them as significantly better able to get along with others. The authors believe the study demonstrates the potential of supportive art therapy to enhance functioning of chronic psychiatric patients in the short run. Empirical attention to long-term gains and to the efficacy of specific forms of art therapy is needed in the future.


Subject(s)
Art Therapy , Mental Disorders/therapy , Adult , Chronic Disease , Female , Humans , Interpersonal Relations , Male , Self Concept
9.
Neurology ; 33(4): 483-8, 1983 Apr.
Article in English | MEDLINE | ID: mdl-6188077

ABSTRACT

We report muscle biopsy abnormalities in four patients with a chronic cholestatic syndrome, low serum vitamin E levels, absent reflexes, mild limb weakness, ataxia, and sensory loss in arms and legs. Skeletal muscle fibers contained multiple autofluorescent inclusions that show strong acid phosphatase and esterase reactivity. By electronmicroscopy, the inclusions lying between myofibrils were membrane-bound dense bodies having characteristics of both lysosomes and lipopigment material. The material was similar to that observed in vitamin E-deficient animals and probably formed in response to disordered intracellular lipid peroxidation.


Subject(s)
Muscles/pathology , Vitamin E Deficiency/pathology , Ceroid/analysis , Child , Child, Preschool , Cholestasis/complications , Humans , Inclusion Bodies/analysis , Inclusion Bodies/ultrastructure , Lipofuscin/analysis , Lysosomes/analysis , Lysosomes/ultrastructure , Muscles/ultrastructure , Neuromuscular Diseases/etiology , Neuromuscular Diseases/pathology , Staining and Labeling , Vitamin E Deficiency/etiology
10.
Arch Neurol ; 39(3): 157-63, 1982 Mar.
Article in English | MEDLINE | ID: mdl-7039564

ABSTRACT

Of four patients with Sjögren's syndrome, three had polymyositis and one had dermatomyositis. In all, deposition of IgG, IgA, IgM, and C3 was observed in muscle by immunofluorescent techniques. Serologic studies revealed elevated levels of serum IgG and IgM, rheumatoid factor, and antinuclear antibody with specificity for SS-A and SS-B antigens. In muscle there was a mononuclear cell infiltrate with plasma cell predominance around small vessels and capillaries. Ultrastructural changes in the vessels included reduplication of the basement membrane, endothelial thickening, and numerous tubuloreticular and dense inclusions. In two patients, electrondense deposits were noted in the microvasculature. This combination of immunoglobulin deposition in muscle, prominent microvascular changes, and characteristic serology suggests that the myositis in Sjögren's syndrome may result from small-vessel injury by autoantibodies or circulating immune complexes.


Subject(s)
Dermatomyositis/immunology , Myositis/immunology , Sjogren's Syndrome/immunology , Adult , Antibodies, Antinuclear/analysis , Autoantibodies/analysis , Biopsy , Complement C3/analysis , Dermatomyositis/diagnosis , Female , Fluorescent Antibody Technique , Humans , Immunoglobulin A/analysis , Immunoglobulin G/analysis , Immunoglobulin M/analysis , Middle Aged , Muscles/pathology , Myositis/diagnosis , Sjogren's Syndrome/diagnosis , Skin/pathology
11.
Invest Ophthalmol Vis Sci ; 19(1): 75-86, 1980 Jan.
Article in English | MEDLINE | ID: mdl-7350137

ABSTRACT

Studies of the retina in 6- and 22-month-old English setters with progressive blindness, ataxia, and muscle weakness demonstrated a marked accumulation of abnormal cytosomes within neurons and retinal pigmented epithelial cells. Ganglion cells contained abundant cytosomes with evenly spaced stacks of membranes; bipolar and amacrine cell cytosomes consisted of dense, amorphous material with closely spaced configurations of light and dark lines; cytosomes within photoreceptor cells contained faintly staining curved profiles. All three cytosomes resembled those previously reported in brain neurons of CCL dogs. In retinal pigmented epithelial cells, there were prominent accumulations of lamellar fragments, either free in the cytoplasm or incorporated into melanin granules. These retinal abnormalities are likely to be related to deficiences of peroxidase and defects of lipid peroxidation. The pathologic and biochemical changes seen in these dogs are similar in many respects to those reported in human patients with Batten disease. As such, these dogs provide a convenient model for the study disease mechanisms and for therapeutic approaches to blindness in Batten disease.


Subject(s)
Dog Diseases/pathology , Pigment Epithelium of Eye/pathology , Retinal Degeneration/veterinary , Animals , Ceroid , Disease Models, Animal , Dog Diseases/genetics , Dogs , Humans , Lipidoses/pathology , Lipofuscin , Neurons/pathology , Pigment Epithelium of Eye/ultrastructure , Retina/pathology , Retina/ultrastructure , Retinal Degeneration/pathology
12.
Neurochem Int ; 1C: 405-26, 1980.
Article in English | MEDLINE | ID: mdl-20487751

ABSTRACT

Human ceroid-lipofuscinosis is marked by blindness, dementia, ataxia, and premature death. A canine model for this disease exists in English setters whose clinical, pathological and biochemical changes resemble the human disorder. In both syndromes, autofluorescent lipopigments, i.e.; lipofuscin and ceroid ("granular", "fingerprint" and/or "curvilinear bodies") are found in the nervous system, viscera, retina, and pigment epithelium (RPE). Retinal neurons of affected animals between 6 and 22 months of age, contain a variety of abnormal intracellular pigment inclusions. Pigment epithelial cells also contain distinctive cytosomes. Electroretinograms from affected animals showed a reduction in b-wave amplitude. Leukocyte, retinal, and RPE peroxidases, were decreased in affected animals, and also showed age-related changes. In the normal canine eye, peroxidase was associated with fractions containing plasma membranes and melanolysosomes. Improved fractionation techniques localized normal peroxidase to "heavy" fractions (1.24-1.28 g/ml), and peroxidase was decreased in these fractions in CCL animals. A new particle containing hexosaminidase, galactosidase, and acid lipase was observed in affected animals. When retinal homogenates from CCL dogs were injected into the vitreous of rabbit eyes they completely abolished the ERG recording. No such change was observed with homogenates from unaffected animals. The accumulation of large numbers of dense bodies in the retina and RPE in dogs with CCL, along with a decrease in peroxidase, suggests an impairment of degradative mechanisms. Furthermore, ceroid appears to be cytotoxic to the retina and RPE. The relationship of these cytotoxic properties to the accumulation of ceroid in the eye, is the subject of our future research.

13.
Pathology ; 9(1): 39-41, 1977 Jan.
Article in English | MEDLINE | ID: mdl-854361

ABSTRACT

Urinary sediment from a patient with the juvenile form of Batten disease was examined by electron microscopy. Membrane bound fingerprint profiles were found which were similar to those previously described in a biopsy from this patient's thyroid gland. These findings might represent a useful diagnostic tool.


Subject(s)
Lipidoses/urine , Humans , Lipidoses/pathology , Microscopy, Electron
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