Alterations of the intestinal microbiome and metabolome in women with rheumatoid arthritis.

Rheumatoid arthritis (RA) is more common in women, and many reports of sex differences have been reported in various aspects of RA. However, there has been a lack of specific research on women's gut flora. To assess the association between the gut flora and RA patients, this study combined the microbiome with metabolomics. Fecal samples from RA patients and healthy controls were collected for 16S rRNA sequencing. Nontargeted liquid chromatography-mass spectrometry was used to detect metabolites in fecal samples. We comprehensively used various analytical methods to reveal changes in intestinal flora and metabolites in female patients. The gut flora of RA patients was significantly different from that of healthy women. The abundance of Bacteroides, Megamonas and Oscillospira was higher in RA patients, while the abundance of Prevotella, Gemmiger and Roseburia was lower than that of healthy women. Gemmiger, Bilophila and Odoribacter represented large differences in microflora between RA and healthy women and could be used as potential microorganisms in the diagnosis. Fatty acid biosynthesis was significantly different between RA patients and healthy women in terms of metabolic pathways. There were different degrees of correlation between the gut flora and metabolites. Lys-Phe-Lys and heptadecasphin-4-enine can be used as potential markers for RA diagnosis. There was an extremely significant positive correlation between Megamonas, Dialister and rheumatoid factors, which was found for the first time. These findings indicated that alterations of these gut microbiome and metabolome may contribute to the diagnosis and treatment of RA patients.

Distributed transverse stress measurement along an optic fiber using polarimetric OFDR.

We report a novel polarimetric optical frequency domain reflectometer (P-OFDR) that can simultaneously measure both space-resolved transverse stresses and light back-reflections along an optic fiber with sub-mm spatial resolution. By inducing transversal stresses and optical back-reflections at multiple points along a length of optic fiber, we demonstrate that our system can unambiguously distinguish the stresses from the back-reflections of a fiber with a fiber length longer than 800 m, a spatial resolution of 0.5 mm, a maximum stress level of up to 200 kpsi (1379 Mpa), a minimum stress of about 10 kpsi (69 Mpa), and a stress measurement uncertainty of 10%. We show that our P-OFDR can clearly identify the locations and magnitudes of the stresses inside a fiber coil induced during a fiber winding process. The P-OFDR can be used for fiber health monitoring for critical fiber links, fiber gyro coil characterization, and other distributed fiber sensing applications.

A hop count based heuristic routing protocol for mobile delay tolerant networks.

Routing in delay tolerant networks (DTNs) is a challenge since it must handle network partitioning, long delays, and dynamic topology. Meanwhile, routing protocols of the traditional mobile ad hoc networks (MANETs) cannot work well due to the failure of its assumption that most network connections are available. In this paper, we propose a hop count based heuristic routing protocol by utilizing the information carried by the peripatetic packets in the network. A heuristic function is defined to help in making the routing decision. We formally define a custom operation for square matrices so as to transform the heuristic value calculation into matrix manipulation. Finally, the performance of our proposed algorithm is evaluated by the simulation results, which show the advantage of such self-adaptive routing protocol in the diverse circumstance of DTNs.

Curcumin suppresses proliferation and invasion in non-small cell lung cancer by modulation of MTA1-mediated Wnt/ß-catenin pathway.

Curcumin, a naturally occurring phenolic compound, has a diversity of antitumor activities. It has been previously demonstrated that curcumin can inhibit the invasion and metastasis of tumors through activation of the tumor suppressor DnaJ-like heat shock protein 40 (HLJ1). However, the specific roles and mechanisms of curcumin in regulating the malignant behaviors of non-small cell lung cancer (NSCLC) cells still remain unclear. In this study, we found that curcumin could inhibit the proliferation and invasion of NSCLC cells and induce G0/G1 phase arrest. Metastasis-associated protein 1 (MTA1) overexpression has been detected in a wide variety of aggressive tumors and plays an important role on cell invasion and metastasis. Our results showed that curcumin could effectively inhibit the MTA1 expression of NSCLC cells. Further research on the subsequent mechanism showed that curcumin inhibited the proliferation and invasion of NSCLC cells through MTA1-mediated inactivation of Wnt/ß-catenin pathway. Wnt/ß-catenin signaling was reported to play a critical cooperative role on promoting lung tumorigenesis. Thus, these investigations provided novel insights into the mechanisms of curcumin on inhibition of NSCLC cell growth and invasion and showed potential therapeutic strategies for NSCLC.