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1.
Front Endocrinol (Lausanne) ; 14: 1054674, 2023.
Article in English | MEDLINE | ID: mdl-37033234

ABSTRACT

Goals: To assess the efficacy and safety of Chinese Medicine Prescription "W-LHIT" in subjects with simple obesity, and to explore its potential mechanism of action. Methods: Thirty-seven patients aged 18 to 60 from Wei-En hospital (Weifang City, Shandong, China), participated in a double blinded, placebo-controlled study. Subjects were randomly divided into 2 groups, 18 in treatment and 19 in placebo group. The treatment group took the "W-LHIT" capsules for two months, while the control group received placebo capsules. Both groups accepted healthy lifestyle education materials. After a 2-month treatment, the placebo group transferred to open-label treatment after unblinding. Results: 72.22% participants in the treatment group lost more than 5% of their body weight, compared with 36.84% in the placebo group (p < 0.001). Body weight loss and body mass index reduction of the treatment group were also significantly higher than those of the placebo group (p < 0.05). These changes were accompanied by increased abundance of Akkermansia muciniphila and Enterococcus faecium, and decreased abundance of Proteobacteria in gut microbiota. Furthermore, the treatment group also showed improvement in obesity-related comorbidities such as hypertension and elevation of liver enzymes. No serious adverse reactions were found during the study period. Weight did not rebound at a follow-up visit 2 months after treatment. Conclusion: W-LHIT significantly improved body weight and comorbid conditions without obvious adverse reaction or rebound weight gain. These effects were associated with increased abundance of probiotics in gut microbiota. W-LHIT may have a potential for treating obesity in conjunction with healthy lifestyle modifications.


Subject(s)
Gastrointestinal Microbiome , Humans , Obesity/complications , Obesity/drug therapy , Weight Loss , Treatment Outcome , Life Style
2.
Ther Adv Med Oncol ; 12: 1758835920965853, 2020.
Article in English | MEDLINE | ID: mdl-33193827

ABSTRACT

BACKGROUND: Hypo-fractionation radiotherapy (HFRT) was considered to be an important treatment for non-small cell lung cancer (NSCLC), but the radiobiological effects of HFRT on NSCLC remain unclear. The aim of this study was to investigate specific biological effect of HFRT on tumor angiogenesis, compared with conventional radiotherapy (CRT). METHODS: The subcutaneous xenograft models and the dorsal skinfold window chamber (DSWC) models of nude mice bearing H460 and HCC827 NSCLC cells were irradiated with doses of 0 Gy (sham group), 22 Gy delivered into 11 fractions (CRT group) or 12 Gy delivered into 1 fraction (HFRT group). At certain time-points after irradiation, the volumes, hypoxic area, coverage rate of pericyte and micro-vessel density (MVD) of the subcutaneous xenograft models were detected, and the tumor vasculature was visualized in the DSMC model. The expressions of phosphorylated signal transducer and activator of transcription (p-STAT3), hypoxia-inducible factor 1-α (HIF-1α), CXCL12 and VEGFA were detected. RESULTS: Compared with the CRT groups, HFRT showed more-efficient tumor growth-suppression, accompanied by a HFRT-induced window-period, during which vasculature was normalized, tumor hypoxia was improved and MVD was decreased. Moreover, during the window-period, the signal levels of p-STAT3/HIF-1α pathway and the expressions of its downstream angiogenic factors (VEGFA and CXCL12) were inhibited by HFRT. CONCLUSION: Compared with CRT, HFRT induced tumor vasculature normalization by rendering the remaining vessels less tortuous and increasing pericyte coverage of tumor blood vessels, thereby ameliorating tumor hypoxia and enhancing the tumor-killing effect. Moreover, HFRT might exert the aforementioned effects through p-STAT3/HIF-1α signaling pathway.

3.
J Hematol Oncol ; 13(1): 142, 2020 Oct 22.
Article in English | MEDLINE | ID: mdl-33092612

ABSTRACT

An amendment to this paper has been published and can be accessed via the original article.

4.
Oncol Lett ; 20(4): 61, 2020 Oct.
Article in English | MEDLINE | ID: mdl-32863894

ABSTRACT

The epidermal growth factor receptor-tyrosine kinase inhibitors (EGFR-TKIs) contribute to an increased response rate, compared with chemotherapy, in patients with inhibitor-sensitive EGFR mutations. The present study evaluated the association between the maximum standardized uptake value (SUVmax) of 18F-fluorodeoxyglucose positron emission tomography-computed tomography (FDG PET/CT), as well as serum carcinoembryonic antigen (CEA) levels and EGFR mutations prior to treatment, in patients with non-small cell lung cancer (NSCLC). Patients with histologically confirmed NSCLC (n=167), who underwent an 18F-FDG PET/CT scan, EGFR mutation analysis and a serum CEA test participated in the present study. Multivariate logistic regression analysis was used to analyze predictors of EGFR mutations. Receiver-operating characteristic (ROC) curve analysis was performed to determine the efficient cut-off value. Survival rate analysis was evaluated according to SUVmax and EGFR mutation status. A decreased SUVmax and an increased CEA level was observed in patients with EGFR-mutations, compared with patients with wild-type primary lesions and metastatic lymph nodes. The exon 19 EGFR mutation was associated with increased SUVmax, compared with the exon 21 L858R mutation. The ROC analysis indicated that an 18F-FDG PET/CT uptake SUVmax >11.5 may be a predictor of the wild-type EGFR genotype and increased CEA levels (CEA >9.4 ng/ml) were associated with EGFR mutations. Furthermore, patients with no smoking history, low SUVmax of the primary tumor, metastatic lymph nodes and a high CEA level were significantly associated with EGFR mutation status. The results of the present study indicated that patients with advanced NSCLC, particularly Chinese patients, with decreased SUVmax and increased CEA levels are associated with EGFR mutations, which may serve as predictors for the EGFR-TKI therapeutic response.

8.
Interact Cardiovasc Thorac Surg ; 26(5): 761-767, 2018 05 01.
Article in English | MEDLINE | ID: mdl-29346581

ABSTRACT

OBJECTIVES: Lung ischaemia-reperfusion injury is a complex pathophysiological process due to the production of reactive oxygen species and the generation of inflammatory reaction. We investigated the protective effects and the corresponding mechanism of melatonin (MT), a potent free-radical scavenger, on lung injury induced by ischaemia-reperfusion in a mouse model. METHODS: Adult male C57BL/6J mice (n = 30) were randomly and equally allocated into 5 groups: sham controls, IR, IR + 10 mg/kg MT, IR + 20 mg/kg MT and IR + 30 mg/kg MT. Lung ischaemia-reperfusion injury was induced by thoracotomy followed by clamping of the left hilum for 1 h and subsequent reperfusion for 2 h. RESULTS: Histological scoring analysis showed that lung parenchymal damage was ameliorated in the melatonin pretreatment groups when compared with the IR group, with the IR + 20 mg/kg MT group showing the strongest effect among the melatonin pretreatment groups. Wet-to-dry weight ratio, detection of malondialdehyde, protein expressions of inflammatory factors (tumour necrosis factor-α, interleukin-1ß, NF-κB and IKK-γ) and apoptotic factors (cleaved caspase-3 and Bax/Bcl-2), as well as TUNEL assay showed changes similar to those of the lung injury scores in all groups. In contrast, the examination of superoxide dismutase showed a pattern contrary to that of the lung injury score in all groups. In addition, immunohistochemistry staining showed that the expressions of the antioxidants glutathione peroxidase and glutathione reductase were increased in the melatonin pretreatment groups. CONCLUSIONS: This study demonstrated that melatonin pretreatment attenuated lung ischaemia-reperfusion injury via inhibition of oxidative stress, inflammation and apoptosis.


Subject(s)
Antioxidants/therapeutic use , Lung Injury/prevention & control , Melatonin/therapeutic use , Reperfusion Injury/prevention & control , Animals , Apoptosis/drug effects , Caspase 3/metabolism , Disease Models, Animal , I-kappa B Kinase/metabolism , Inflammation/pathology , Inflammation/prevention & control , Interleukin-1beta/metabolism , Lung Injury/pathology , Male , Malondialdehyde/metabolism , Mice , Mice, Inbred C57BL , NF-kappa B/metabolism , Oxidative Stress/drug effects , Random Allocation , Reperfusion Injury/pathology , Superoxide Dismutase/metabolism , Tumor Necrosis Factor-alpha/metabolism
9.
J Hematol Oncol ; 10(1): 125, 2017 06 19.
Article in English | MEDLINE | ID: mdl-28629431

ABSTRACT

BACKGROUND: Brain metastasis (BM) is associated with poor prognosis in patients with non-small cell lung cancer (NSCLC). Recent studies demonstrated that microRNA-330-3p (miR-330-3p) was involved in NSCLC brain metastasis (BM). However, the exact parts played by miR-330-3p in BM of NSCLC remain unknown. Discovery and development of biomarkers and elucidation of the mechanism underlying BM in NSCLC is critical for effective prophylactic interventions. Here, we evaluated the expression and biological effects of miR-330-3p in NSCLC cells and explored the underlying mechanism of miR-330-3p in promoting cell migration and invasion in NSCLC. METHODS: Stable over-expression and knockdown of miR-330-3p in NSCLC cells was constructed with lentivirus. Expression levels of miR-330-3p in NSCLC cells were quantified by quantitive real-time PCR (qRT-PCR). The effects of miR-330-3p on NSCLC cells were investigated using assays of cell viability, migration, invasion, cell cycle, apoptosis, western blotting, immunohistochemical, and immunofluorescence staining. A xenograft nude mouse model and in situ brain metastasis model were used to observe tumor growth and brain metastasis. The potential target of miR-330-3p in NSCLC cells was explored using the luciferase reporter assay, qRT-PCR, and western blotting. The miR-330-3p targets were identified using bioinformatics analysis and verified by luciferase reporter assay. The correlation between GRIA3 and DNA methyltransferase (DNMT) 1 and DNMT3A was tested by RT-PCR, western blotting, and co-immunoprecipitation (IP). RESULTS: miR-330-3p was significantly up-regulated in NSCLC cell lines. MTT assay, transwell migration, and invasion assays showed that miR-330-3p promoted the growth, migration, and invasion of NSCLC cells in vitro and induced tumor growth and metastasis in vivo. Luciferase reporter assays showed that GRIA3 was a target of miR-330-3p. qRT-PCR and western blotting exhibited that miR-330-3p promoted the growth, invasion, and migration of NSCLC cells by activating mitogen-activated protein kinase (MAPK)/extracellular-regulated protein kinases (ERK) signaling pathway. Furthermore, miR-330-3p up-regulated the total DNA methylation in NSCLC cells, and co-IP-demonstrated GRIA3 was directly related with DNMT1 and DNMT3A. CONCLUSIONS: miR-330-3p promoted the progression of NSCLC and might be a potential target for the further research of NSCLC brain metastasis.


Subject(s)
Brain Neoplasms/genetics , Brain Neoplasms/secondary , Carcinoma, Non-Small-Cell Lung/genetics , Lung Neoplasms/genetics , MAP Kinase Signaling System , MicroRNAs/genetics , Receptors, AMPA/genetics , Animals , Brain Neoplasms/enzymology , Brain Neoplasms/pathology , Carcinoma, Non-Small-Cell Lung/enzymology , Carcinoma, Non-Small-Cell Lung/pathology , Cell Cycle , Cell Line, Tumor , Cell Movement , Cell Proliferation , DNA Methylation , Enzyme Activation , Female , Gene Expression Regulation, Neoplastic , Humans , Lung Neoplasms/enzymology , Lung Neoplasms/pathology , Mice , Mice, Inbred BALB C , Mice, Nude , Neoplasm Invasiveness/genetics , Neoplasm Invasiveness/pathology , Up-Regulation
10.
Arch Virol ; 162(3): 823-833, 2017 Mar.
Article in English | MEDLINE | ID: mdl-27858288

ABSTRACT

Porcine reproductive and respiratory syndrome (PRRS) is considered one of the most devastating swine diseases worldwide, resulting in immense economic losses. PRRS virus (PRRSV) is divided into two major genotypes, European (type 1) and the North American (type 2). Type 1 PRRSV have recently emerged in Fujian province (South China), and this might have a significant impact on the Chinese pig industry. From 2013 to 2014, two type 1 PRRSV strains, named FJEU13 and FJQEU14, were isolated from piglets and sows with respiratory problems and reproductive disorders in Fujian province. The full genome length of the two isolates was 14,869-15,062 nucleotides (nt), excluding the poly(A) tail. These isolates shared 86.0-89.9% sequence identity with the prototypic strains Lelystad virus (LV) and 82.8-92% with Chinese type 1 PRRSV strains, but only 59.9-60.1% with the North American reference strain VR-2332. However, they were 82.9% identical to each other. Nonstructural protein 2 (Nsp2) and ORF3-ORF5 were the most variable regions when compared to other type 1 PRRSV strains. Nsp2 and ORF3 contained multiple discontinuous deletions and a 204-bp deletion in NSP2 in isolate FJQEU14, which has never been described in other Chinese type 1 PRRSV strains. All of these results might be useful for understanding the epidemic status of type 1 PRRSV in China.


Subject(s)
Genome, Viral , Porcine Reproductive and Respiratory Syndrome/virology , Porcine respiratory and reproductive syndrome virus/genetics , Porcine respiratory and reproductive syndrome virus/isolation & purification , Amino Acid Sequence , Animals , China , Genetic Variation , Genomics , Genotype , Molecular Sequence Data , Open Reading Frames , Phylogeny , Porcine respiratory and reproductive syndrome virus/chemistry , Porcine respiratory and reproductive syndrome virus/classification , RNA, Viral/genetics , Sequence Alignment , Swine , Viral Proteins/chemistry , Viral Proteins/genetics
11.
Sci Rep ; 6: 29067, 2016 07 01.
Article in English | MEDLINE | ID: mdl-27364081

ABSTRACT

The yellow peach moth (YPM), Conogethes punctiferalis (Guenée), is a multivoltine insect pest of crops and fruits. Antennal-expressed receptors are important for insects to detect olfactory cues for host finding, mate attraction and oviposition site selection. However, few olfactory related genes were reported in YPM until now. In the present study, we sequenced and characterized the antennal transcriptomes of male and female YPM. In total, 15 putative odorant binding proteins (OBPs), 46 putative odorant receptors (ORs) and 7 putative ionotropic receptors (IRs) were annotated and identified as olfactory-related genes of C. punctiferalis. Further analysis of RT-qPCR revealed that all these olfactory genes are primarily or uniquely expressed in male and female antennae. Among which, 3 OBPs (OBP4, OBP8 and PBP2) and 4 ORs (OR22, OR26, OR44 and OR46) were specially expressed in male antennae, whereas 4 ORs (OR5, OR16, OR25 and OR42) were primarily expressed in female antennae. The predicted protein sequences were compared with homologs in other lepidopteran species and model insects, which showed high sequence homologies between C. punctiferalis and O. furnacalis. Our work allows for further functional studies of pheromone and general odorant detection genes, which might be meaningful targets for pest management.


Subject(s)
Arthropod Antennae/physiology , Olfactory Perception/genetics , Smell/genetics , Transcriptome/genetics , Animals , Female , Gene Expression Regulation , Insect Proteins/genetics , Male , Moths/genetics , Moths/physiology , Olfactory Perception/physiology , Pheromones/genetics , Phylogeny , Receptors, Odorant/genetics , Smell/physiology
12.
Zhongguo Zhong Yao Za Zhi ; 41(17): 3181-3185, 2016 Sep.
Article in Chinese | MEDLINE | ID: mdl-28920368

ABSTRACT

The application of ArcGIS and Maxent modelto analyze the ecological suitability of Gardenia jasminoides.Taking 85 batches of Gardenia as the basis of analysis, the selection of ecological factors for the growth of Gardenia. The results showed that the average precipitation in April, the average precipitation in November and the average precipitation in August were the most important factors affecting the growth of Gardenia. The relative concentration of Gardenia suitable growth region,north to the south of Shaanxi province, south of Henan, central Anhui, south to the north of Hainan province, west to central Sichuan province, east of Zhejiang coastal area, northeast of Taiwan.


Subject(s)
Gardenia/growth & development , China , Climate , Ecology , Geographic Information Systems
13.
Ying Yong Sheng Tai Xue Bao ; 27(6): 1942-1952, 2016 Jun.
Article in Chinese | MEDLINE | ID: mdl-29737703

ABSTRACT

With the continuous drought stress treatment to 12 pot-grown watermelon genotypes originated from different regions, the influence of drought stress on plant height, root length, fresh mass and dry mass was studied, and the physiological responses of these genotypes to drought stress were compared. Drought resistance of these watermelon genotypes was preliminarily determined according to the drought injury, and then confirmed by membership function evaluation method. We found that the watermelon genotypes exhibited great difference in drought tolerance based on the occurrence of drought injury and the degree of injury severity. Drought stress reduced plant height, shoot- and root- fresh mass as well as shoot- and root- dry mass, while increased the root/shoot ratio for most genotypes; but for root length and root dry mass, it had both positive and negative effects depending on the tested genotype. Compared with the control, all drought-treated watermelon genotypes exhibited a decline in leaf relative water content and chlorophyll content, as well as increases in MDA, H2O2, O2-· and proline contents. Different watermelon genotypes displayed diversity in soluble protein content and antioxidant enzyme activity. Our results showed that three wild watermelon genotypes including M20, Y-2, and KY-3 were drought tolerant while Y34, 04-1-2 and Golden Girl were drought sensitive, and the rest genotypes were among the medium.


Subject(s)
Citrullus/physiology , Droughts , Stress, Physiological , Water/physiology , Chlorophyll , Citrullus/genetics , Genotype , Plant Leaves , Plant Roots
14.
Zhongguo Shi Yan Xue Ye Xue Za Zhi ; 23(3): 838-42, 2015 Jun.
Article in Chinese | MEDLINE | ID: mdl-26117046

ABSTRACT

OBJECTIVE: To study the interactions between hemoglobin (Hb) and other proteins within human erythrocytes by using the electrophoresis release test (ERT) and co-immunoprecipitation. METHODS: First, the fresh normal adult anti-coagulated whole blood was washed to prepare packed RBCs, which were further prepared to erythrocyte suspension and hemolysate.The erythrocyte suspension and hemolysate were analyzed by the electrophoresis release test (ERT) at the same time, and then the band of HbA of erythrocyte sample (RA) and the corresponding band of hemolysate sample (HA) were cut out from the gel and were enriched by freeze-thaw method. Then, the samples were bound with hemoglobin ß antibody (37-8) AC, the complexes were separated through 5%-12% SDS-PAGE followed by Q-TOF. RESULTS: Five bands were found in the gel, each of which was treated by hemoglobin ß antibody (37-8) AC, the protein bands of 16,20,22,28 and 50 kD were emerged in RA, HA and RBC lysis.The bands were identified by MS, and the results showed that these bands were hemoglobin, band 3, peroxiredoxin2 (Prx2), band 3 and ß-actin, band 3 respectively. CONCLUSION: HbA may interact with Prx2, Band 3 and ß-actin, and then the complexes are formed with each other within erythrocytes.


Subject(s)
Electrophoresis, Polyacrylamide Gel , Immunoprecipitation , Erythrocytes , Hemoglobins , Humans , Proteins
15.
Shanghai Kou Qiang Yi Xue ; 23(4): 427-30, 2014 Aug.
Article in Chinese | MEDLINE | ID: mdl-25338792

ABSTRACT

PURPOSE: To evaluate the effect of impressions made by agar/alginate compared with silicon rubber material. METHODS: Five moulds of bi-root canal molar, with different root canal angles of 0°, 10°, 20°, 30° and 40° were made. Twenty impressions were made for each mould with either silicon rubber or agar/alginate. The effects of two methods were evaluated and the influence of angles between root canals on the impression methods was analyzed. The data was analyzed with SPSS16.0 software package. RESULTS: Significant difference of integrality rate (IR) of impression was not found in silicon rubber group among different angle groups (P>0.05). IR of 40° group (60%) was significantly lower than that of 0°(95%) and general IR (83%) when using agar (P<0.05). IR of agar (60%) was statistically lower than silicon rubber (90%) in 40° group (P<0.05).The success rate of agar method was significantly lower than silicon rubber method in 40° group. The general IR of agar (83%) was significantly lower than that of silicon rubber (95%) (P<0.05). A significant negative correlation was observed between root canal angles and IR (r=-0.901,P=0.037). CONCLUSIONS: Silicon rubber impression materials are suggested for impression taking when root canal angles are over 30° in molars, while agar/alginate impression method is qualified for smaller angles. The general IR of silicon rubber material is better than that of agar when taking the impression of post and core in molar.


Subject(s)
Agar , Dental Pulp Cavity , Alginates , Dental Impression Technique , Glucuronic Acid , Hexuronic Acids , Humans , Molar , Root Canal Therapy
16.
Acta Crystallogr D Biol Crystallogr ; 70(Pt 2): 421-35, 2014 Feb.
Article in English | MEDLINE | ID: mdl-24531476

ABSTRACT

Dual-specificity protein phosphatases (DUSPs), which dephosphorylate both phosphoserine/threonine and phosphotyrosine, play vital roles in immune activation, brain function and cell-growth signalling. A family-wide structural library of human DUSPs was constructed based on experimental structure determination supplemented with homology modelling. The catalytic domain of each individual DUSP has characteristic features in the active site and in surface-charge distribution, indicating substrate-interaction specificity. The active-site loop-to-strand switch occurs in a subtype-specific manner, indicating that the switch process is necessary for characteristic substrate interactions in the corresponding DUSPs. A comprehensive analysis of the activity-inhibition profile and active-site geometry of DUSPs revealed a novel role of the active-pocket structure in the substrate specificity of DUSPs. A structure-based analysis of redox responses indicated that the additional cysteine residues are important for the protection of enzyme activity. The family-wide structures of DUSPs form a basis for the understanding of phosphorylation-mediated signal transduction and the development of therapeutics.


Subject(s)
Dual-Specificity Phosphatases/chemistry , Dual-Specificity Phosphatases/classification , Enzyme Inhibitors/chemistry , Phylogeny , Catalytic Domain , Crystallography, X-Ray , Cysteine/chemistry , Dual-Specificity Phosphatases/antagonists & inhibitors , Dual-Specificity Phosphatases/genetics , Escherichia coli/genetics , Escherichia coli/metabolism , Humans , Hydrolysis , Models, Molecular , Oxidation-Reduction , Phosphoserine/chemistry , Phosphothreonine/chemistry , Phosphotyrosine/chemistry , Protein Structure, Secondary , Recombinant Proteins/chemistry , Recombinant Proteins/classification , Recombinant Proteins/genetics , Signal Transduction , Structural Homology, Protein , Substrate Specificity
17.
Res Vet Sci ; 96(2): 283-7, 2014 Apr.
Article in English | MEDLINE | ID: mdl-24569297

ABSTRACT

Mucosal immunization is advantageous over other routes of antigen delivery because it can induce both mucosal and systemic immune responses. In this study, we have developed fimbriae protein of enterotoxigenic Escherichia coli (ETEC) F41 was stably expressed on the surface Lactobacillus casei 525. The method of expressing vaccine antigens in L. casei induces both systemic and mucosal immunity after oral or intranasal administration. We demonstrate that an oral or intranasal vaccine based on live recombinant L. casei 525 protects infant mice from ETEC F41 infection. This platform technology can be applied to design oral or intranasal vaccine delivery vehicles against several microbial pathogens.


Subject(s)
Enterotoxigenic Escherichia coli/immunology , Escherichia coli Infections/immunology , Escherichia coli Vaccines/immunology , Fimbriae, Bacterial/immunology , Vaccination/methods , Administration, Intranasal , Administration, Oral , Animals , Animals, Newborn , Blotting, Western , Enzyme-Linked Immunosorbent Assay , Escherichia coli Infections/microbiology , Escherichia coli Infections/prevention & control , Escherichia coli Vaccines/administration & dosage , Female , Immunoglobulin A/analysis , Immunoglobulin G/blood , Mice , Mice, Inbred BALB C , Random Allocation , Specific Pathogen-Free Organisms , Vaccination/standards
18.
Arch Virol ; 158(8): 1811-6, 2013 Aug.
Article in English | MEDLINE | ID: mdl-23525729

ABSTRACT

To more fully understand the extent of genetic diversity of PRRSV in China, we analyzed the Nsp2 and ORF5 gene sequences of 35 representative PRRSV isolates from 2008 to 2012. Sequence analysis revealed that the Nsp2 and ORF5 genes have undergone genetic variation. Furthermore, the isolate FJLYDX04 contains five insertions at positions 599 to 603 and is the first isolate from China reported to have an insertion in Nsp2. Our results suggest that the highly pathogenic PRRSV has become the dominant strain in China and that Chinese PRRSV has undergone rapid evolution and can circumvent immune responses induced by currently used vaccines.


Subject(s)
Genetic Variation , Porcine Reproductive and Respiratory Syndrome/virology , Porcine respiratory and reproductive syndrome virus/isolation & purification , Viral Envelope Proteins/genetics , Viral Nonstructural Proteins/genetics , Animals , China , Cluster Analysis , Evolution, Molecular , Molecular Sequence Data , Phylogeny , Porcine respiratory and reproductive syndrome virus/genetics , Sequence Analysis, DNA , Swine
19.
Mol Cell Probes ; 27(3-4): 149-52, 2013.
Article in English | MEDLINE | ID: mdl-23524252

ABSTRACT

A multiplex polymerase chain reaction (PCR) was designed for the simultaneous detection of three viruses involved in reproductive and respiratory failure in pigs: porcine reproductive and respiratory syndrome virus (PRRSV), classical swine fever virus (CSFV) and porcine circovirus type 2 (PCV-2). Each target produced a specific amplicon with a size of 718 bp (PRRSV), 288 bp (CSFV), or 466 bp (PCV-2). The sensitivity of the multiplex PCR using purified plasmid constructs containing the specific viral target fragments was 2.0 × 10(4), 2.5 × 10(3), and 6.0 × 10(2) copies for PRRSV, CSFV, and PCV-2, respectively. Non-specific reactions were not observed when other viruses, bacteria, and PK-15/Marc-145 cells were used to assess the multiplex PCR. Among 82 clinical samples from Fujian province, co-infection by PRRSV and CSFV was 12.19%, co-infection by PRRSV and PCV-2 was 21.95%, CSFV and PCV-2 was 13.41%, and co-infection by the three viruses was 3.66%. In conclusion, the multiplex PCR should be useful for routine molecular diagnosis and epidemiology. The multiplex PCR was effective in detecting various combinations of one or more of these viruses in pig specimens.


Subject(s)
Circovirus/genetics , Classical Swine Fever Virus/genetics , Multiplex Polymerase Chain Reaction/methods , Porcine respiratory and reproductive syndrome virus/genetics , Animals , RNA, Viral/analysis , Swine , Swine Diseases/virology
20.
Exp Mol Med ; 44(7): 413-23, 2012 Jul 31.
Article in English | MEDLINE | ID: mdl-22573111

ABSTRACT

Homophilic interaction of the L1 family of cell adhesion molecules plays a pivotal role in regulating neurite outgrowth and neural cell networking in vivo. Functional defects in L1 family members are associated with neurological disorders such as X-linked mental retardation, multiple sclerosis, low-IQ syndrome, developmental delay, and schizophrenia. Various human tumors with poor prognosis also implicate the role of L1, a representative member of the L1 family of cell adhesion molecules, and ectopic expression of L1 in fibroblastic cells induces metastasis-associated gene expression. Previous studies on L1 homologs indicated that four N-terminal immunoglobulin-like domains form a horseshoe-like structure that mediates homophilic interactions. Various models including the zipper, domain-swap, and symmetry-related models are proposed to be involved in structural mechanism of homophilic interaction of the L1 family members. Recently, cryo-electron tomography of L1 and crystal structure studies of neurofascin, an L1 family protein, have been performed. This review focuses on recent discoveries of different models and describes the possible structural mechanisms of homophilic interactions of L1 family members. Understanding structural mechanisms of homophilic interactions in various cell adhesion proteins should aid the development of therapeutic strategies for L1 family cell adhesion molecule-associated diseases.


Subject(s)
Neural Cell Adhesion Molecule L1/chemistry , Neural Cell Adhesion Molecule L1/metabolism , Neurites , Protein Interaction Domains and Motifs , Cell Adhesion , Crystallography, X-Ray , Escherichia coli , Humans , Immunoglobulins/chemistry , Neurites/chemistry , Neurites/metabolism , Protein Conformation
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