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1.
Pol J Microbiol ; 73(1): 29-38, 2024 Mar 01.
Article in English | MEDLINE | ID: mdl-38437465

ABSTRACT

Fungal diseases form perforated disease spots in tobacco plants, resulting in a decline in tobacco yield and quality. The present study investigated the antagonistic effect of Bacillus subtilis CTXW 7-6-2 against Rhizoctonia solani, its ability to promote the growth of tobacco seedlings, and the expression of disease resistance-related genes for efficient and eco-friendly plant disease control. Our results showed that CTXW 7-6-2 had the most vigorous growth after being cultured for 96 h, and its rate of inhibition of R. solani growth in vitro was 94.02%. The volatile compounds produced by CTXW 7-6-2 inhibited the growth of R. solani significantly (by 96.62%). The fungal growthinhibition rate of the B. subtilis CTXW 7-6-2 broth obtained after high-temperature and no-high-temperature sterile fermentation was low, at 50.88% and 54.63%, respectively. The lipopeptides extracted from the B. subtilis CTXW 7-6-2 fermentation broth showed a 74.88% fungal growth inhibition rate at a concentration of 100 mg/l. Scanning and transmission electron microscopy showed some organelle structural abnormalities, collapse, shrinkage, blurring, and dissolution in the R. solani mycelia. In addition, CTXW 7-6-2 increased tobacco seedling growth and improved leaf and root weight compared to the control. After CTXW 7-6-2 inoculation, tobacco leaves showed the upregulation of the PDF1.2, PPO, and PAL genes, which are closely related to target spot disease resistance. In conclusion, B. subtilis CTXW 7-6-2 may be an efficient biological control agent in tobacco agriculture and enhance plant growth potential.


Subject(s)
Bacillus subtilis , Nicotiana , Bacillus subtilis/genetics , Disease Resistance , Rhizoctonia
2.
Basic & Clinical Medicine ; (12): 451-457, 2018.
Article in Chinese | WPRIM (Western Pacific) | ID: wpr-693921

ABSTRACT

Objective To investigate the effect of TRIM11(tripartite motif-containing protein 11) target regulating miR-24-3p on the proliferation and invasion of breast cancer cells,and potentiol relation between TRIM11 high ex-pression and the prognosis of breast cancer. Methods Immunohistochemical method was used to detect the expres-sion of TRIM11 in 31 cases of breast cancer and 31 cases of adjacent breast cancer normal tissues. The siRNA TRIM11 lentivirus and miR-24-3p lentivirus were transfected into human breast cancer MCF7 cell lines,observing the correlation expression of TRIM11 and miR- 24- 3p mRNA and protein by using real-time fluorescence quantitative PCR (RT-qPCR) and Western bolt,luciferase reporter experiments were used to verify that the miR-24-3p as a direct target of TRIM11,MTT and Transwell were used to investigate the cells proliferation,activity and invasion. Results The expression of TRIM11 in breast cancer tissues or MCF7 cells was significantly higher (P<0.05),and TRIM11 high expression predicts poor prognosis of breast cancer(P<0.05). siRNA TRIM11 significantly inhibited the expression of TRIM11 in MCF7 cells,moreover,which inhibited the MCF7 cells proliferation,viability and invasion (P<0.05). miR-24-3p significantly reduced 3′-UTR TRIM11 luciferase activity in wild-type (P<0.05),but no effect was found in mutant type.The expression of miR-24-3p was decreased,miR-24-3p and TRIM11 mRNA expression was negatively correlated in MCF7 cells(P<0.05),miR-24-3p inhibition protein and mRNA ex-pression of TRIM11 in MCF7 cells,and inhibit MCF7 cells proliferation,viability and invasion (P<0.05). Conclu-sions The expression of TRIM11 is up-regulated in breast cancer and cells,promote the proliferation and invasion of breast cancer cells though regulating miR-24-3p expression,TRIM11 high expression predicts poor prognosis of breast cancer,TRIM11/miR-24-3p axis is expected to become a new target for treatment of breast cancer.

3.
Article in English | WPRIM (Western Pacific) | ID: wpr-773609

ABSTRACT

Fibroblast-like synoviocytes (FLS) play a pivotal role in Rheumatoid arthritis (RA) pathogenesis through aggressive migration and invasion. Madecassoside (Madec), a triterpenoid saponin present in Centella asiatica herbs, has a potent anti-inflammatory effect. In the present study, Madec exerted an obvious therapeutic effect in reversing the histological lesions in adjuvant-induced arthritis (AIA) rats. To recognize the anti-rheumatoid potentials of Madec, we further investigated whether Madec interfered with FLS invasion and metalloproteinase (MMP) expression. In cultures of primary FLS isolated from the AIA rats, Madec (10 and 30 μmol·L) was proven to considerably inhibit migration and invasion of FLS induced by interleukin 1β (IL-1β), but exhibiting no obvious effect on cell proliferation. Madec repressed IL-1β-triggered FLS invasion by prohibiting the expression of MMP-13. Additionally, Madec suppressed MMP-13 transcription via inhibiting the MMP-13 promoter-binding activity of NF-κB. Our results further showed that Madec down-regulated the translocation and phosphorylation of NF-κB as demonstrated by Western blotting and immunofluorescence assays. In conclusion, our results suggest that Madec exerts anti-RA activity via inhibiting the NF-κB/MMP-13 pathway.


Subject(s)
Animals , Rats , Antirheumatic Agents , Chemistry , Pharmacology , Therapeutic Uses , Arthritis, Experimental , Drug Therapy , Pathology , Cell Movement , Cell Nucleus , Metabolism , Cells, Cultured , Gene Expression Regulation, Enzymologic , Interleukin-1beta , Pharmacology , Matrix Metalloproteinase 13 , Genetics , NF-kappa B , Genetics , Metabolism , Phosphorylation , Protein Transport , Signal Transduction , Synoviocytes , Metabolism , Transcriptional Activation , Triterpenes , Chemistry , Pharmacology , Therapeutic Uses
4.
Article in English | WPRIM (Western Pacific) | ID: wpr-812398

ABSTRACT

Fibroblast-like synoviocytes (FLS) play a pivotal role in Rheumatoid arthritis (RA) pathogenesis through aggressive migration and invasion. Madecassoside (Madec), a triterpenoid saponin present in Centella asiatica herbs, has a potent anti-inflammatory effect. In the present study, Madec exerted an obvious therapeutic effect in reversing the histological lesions in adjuvant-induced arthritis (AIA) rats. To recognize the anti-rheumatoid potentials of Madec, we further investigated whether Madec interfered with FLS invasion and metalloproteinase (MMP) expression. In cultures of primary FLS isolated from the AIA rats, Madec (10 and 30 μmol·L) was proven to considerably inhibit migration and invasion of FLS induced by interleukin 1β (IL-1β), but exhibiting no obvious effect on cell proliferation. Madec repressed IL-1β-triggered FLS invasion by prohibiting the expression of MMP-13. Additionally, Madec suppressed MMP-13 transcription via inhibiting the MMP-13 promoter-binding activity of NF-κB. Our results further showed that Madec down-regulated the translocation and phosphorylation of NF-κB as demonstrated by Western blotting and immunofluorescence assays. In conclusion, our results suggest that Madec exerts anti-RA activity via inhibiting the NF-κB/MMP-13 pathway.


Subject(s)
Animals , Rats , Antirheumatic Agents , Chemistry , Pharmacology , Therapeutic Uses , Arthritis, Experimental , Drug Therapy , Pathology , Cell Movement , Cell Nucleus , Metabolism , Cells, Cultured , Gene Expression Regulation, Enzymologic , Interleukin-1beta , Pharmacology , Matrix Metalloproteinase 13 , Genetics , NF-kappa B , Genetics , Metabolism , Phosphorylation , Protein Transport , Signal Transduction , Synoviocytes , Metabolism , Transcriptional Activation , Triterpenes , Chemistry , Pharmacology , Therapeutic Uses
5.
Zhonghua Zhong Liu Za Zhi ; 27(9): 565-6, 2005 Sep.
Article in Chinese | MEDLINE | ID: mdl-16438859

ABSTRACT

OBJECTIVE: To investigate the diagnostic value of dual-head (18)F-fluorodeoxyglucose ((18)F-FDG) imaging in metastatic lesion with unknown primary tumour (UPT). METHODS: Seventy patients with UPT underwent dual-head (18)F-FDG imaging after iv (18)F-FDG 1.85 MBq/kg. The primary tumour was diagnosed according to the FDG uptake and T/N value. RESULTS: Of the 70 patients, the primary tumour was identified by positive FDG imaging and finally confirmed pathologically in 58 patients (82.9%), and 12 patients had a negative FDG imaging (17.1%). Forty-two of the 58 positive patients were found to have lung cancer (72.4%). Among the 12 negative patients, their primary tumour was then identified by other diagnostic procedures in 5 patients (41.7%), in 1 patient, the primary site was detected during follow-up, however, the primary tumour was never detected in the rest 6 patients. CONCLUSION: Dual-probe (18)F-FDG imaging is a simple, quick, non-invasive and sensitive technique with an accuracy over 80% in the diagnosis of unknown primary tumour. The lung is found to be the most frequent primary site. Dual-probe (18)F-FDG imaging can be recommended as the first diagnostic choice for UPT.


Subject(s)
Fluorodeoxyglucose F18 , Lung Neoplasms/diagnostic imaging , Lymph Nodes/diagnostic imaging , Neoplasms, Unknown Primary/diagnostic imaging , Positron-Emission Tomography , Female , Humans , Lung Neoplasms/pathology , Lymph Nodes/pathology , Lymphatic Metastasis , Male , Neoplasms, Unknown Primary/pathology
6.
Ai Zheng ; 23(10): 1222-4, 2004 Oct.
Article in Chinese | MEDLINE | ID: mdl-15473941

ABSTRACT

BACKGROUND & OBJECTIVE: Nasopharynx applicator used in intracavitary brachytherapy plays an important role in the radiotherapy of nasopharyngeal carcinoma (NPC), its quality affects the efficiency of treatment. This study was to design a new applicator for clinical use. METHODS: An inexpensive, reusable, and flexible latex nasopharynx applicator was designed. An air bag was placed at 15 mm from the foreside of the applicator, clung to the tube. The edge of air bag is tangent to the axis of tube. When the bag was full of air, the tube would hunch reversely,close to nasopharyngeal vault. After introduced into nasopharynx through middle nasal meatus, the applicator could be fixed in suitable position by its rotation, and air bag regulation, and confirmed its position by simulation. RESULTS: A total of 221 patients with NPC were treated with external beam radiation therapy in our hospital, and boosted HDR brachytherapy using this new applicator. The response rate was 92.6% in the primary tumor group (200/216), and 100% in the recurrent tumor group (5/5). Mucosal necrosis in the posterior or anterior wall of nasopharynx occurred in 5 patients, 8 patients experienced nasal congestion and nasal synechia. CONCLUSIONS: This new nasopharynx applicator is easy to operate, painless, and well dosage-distributed. Mucosal necrosis is likely due to higher fractional dose.


Subject(s)
Brachytherapy/instrumentation , Carcinoma, Squamous Cell/radiotherapy , Nasopharyngeal Neoplasms/radiotherapy , Adolescent , Adult , Aged , Brachytherapy/methods , Cobalt Radioisotopes/therapeutic use , Equipment Design , Equipment Safety , Female , Humans , Male , Middle Aged , Treatment Outcome
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