ABSTRACT
Graphene oxide has been widely used in biological applications because of good biocompatibility. In the present work, graphene oxide was synthesized successfully and was employed to immobilize glutaryl-7-aminocephalosporanic acid acylase (GLA). The immobilization conditions like pH, temperature, immobilization time, and enzyme/carrier ratio were investigated. Under the optimal immobilization conditions, the immobilization yield and efficiency were 86% and 52%, respectively. The characterization of the immobilized GLA was performed and compared with the free enzyme. The results indicated that the stability of the immobilized GLA was 1.7-folds than that of the free enzyme, and the kinetic parameters were also calculated. All these results show the potential industrial applications of the immobilized GLA in the production of 7-aminocephalosporinal acid (7-ACA).
