Subject(s)
Rectal Neoplasms , Humans , Rectal Neoplasms/surgery , Treatment Outcome , China/epidemiologyABSTRACT
BACKGROUND: There is no consensus that single-incision laparoscopic surgery splenectomy (SILS-SP) is on a par with conventional multiport laparoscopic surgery splenectomy (CMLS-SP). AIMS: The aim of this systematic review and meta-analysis was to assess feasibility and safety of SILS-SP when compared with CMLS-SP. MATERIALS AND METHODS: Eligible articles were identified by searching several databases including PubMed, EMBASE, CNKI (China) and the Cochrane Library, up until February 2016. Studies were reviewed independently and rated by Newcastle-Ottawa Quality Assessment Scale. Evaluated outcomes were complications, operative time, post-operative hospital stay, blood loss, starting diet, post-operative pain scores, conversion and analgesic requirements. RESULTS: Ten retrospective studies met the eligibility criteria. Overall, there was no significant difference between SILS-SP and CMLS-SP in complications, operative time, post-operative hospital stay, blood loss, starting diet, post-operative pain scores, conversion and analgesic requirements. CONCLUSIONS: SILS-SP is feasible and safe in certain patients, with no obvious advantages over CMLS-SP. Therefore, it may be considered an alternative to CMLS-SP. We await high-quality, double-blind RCTs. These should include clear statements on standard scores of post-operative pain and cosmetic results, longer follow-up assessment and cost-benefit analysis.
ABSTRACT
AIM: To explore the effect and significance of inhibitor of growth 1 (ING1) gene in carcinogenesis and progression of human sporadic colorectal cancer. METHODS: mRNA expression, mutation, and loss of heterozygosity (LOH) of ING1 gene in 35 specimens of sporadic colorectal cancer tissues and the matched normal mucous membrane tissues were detected by semi-quantitative reverse transcriptase-polymerase chain reaction (RT-PCR), PCR-single strain conformation polymorphism (PCR-SSCP) and PCR-simple sequence length polymorphism (PCR-SSLP) using microsatellite markers, respectively. RESULTS: The average ratios of light intensities of p33(ING1b) and p47(ING1a) mRNA expression in the cancerous tissues were significantly lower than those in normal tissues. The difference between the two mRNA splices was not significant in the matched tissues. In addition, the ratios of light intensities of p33(ING1b) and p47(ING1a) mRNA expression in the cancerous tissues of Dukes' stages C and D were significantly lower than those in cancerous tissues of Dukes' stages A and B. However, no mutation of ING1 gene was detected in all 35 cases; only 4 cases of LOH (11.4%) were found. CONCLUSION: p33(ING1b) and p47(ING1a) mRNA expressions are closely related with the carcinogenesis and progression of human sporadic colorectal cancer. No mutation of ING1 gene is found, and there are only few LOH in sporadic colorectal cancers. These might not be the main reasons for the down regulation of ING1 expression. Its low expression may happen in transcription or post-transcription.
Subject(s)
Colorectal Neoplasms/genetics , Gene Expression Regulation, Neoplastic , Intracellular Signaling Peptides and Proteins/genetics , Nuclear Proteins/genetics , Tumor Suppressor Proteins/genetics , Humans , Inhibitor of Growth Protein 1 , Loss of Heterozygosity , Polymorphism, Single-Stranded ConformationalABSTRACT
BACKGROUND & OBJECTIVE: Inhibitor of growth 1 (ING(1)) gene has been identified as a novel tumor suppressor gene. Its over-expression can inhibit cell growth, and induce cell apoptosis. This study was to explore the effect and significance of ING(1) gene in occurrence and progression of sporadic colorectal cancer. METHODS: The expression, mutation, and loss of heterozygosity (LOH) of ING(1) gene in 46 specimens of sporadic colorectal cancer tissues and adjuvant normal mucous membrane tissues were detected by semi-quantitative reverse transcription-polymerase chain reaction (RT-PCR), PCR-single strain conformation polymorphism (PCR-SSCP), and microsatellite markers, respectively. RESULTS: (1)The average ratios of light density of p33/ING(1) mRNA, and p47/ING(1) mRNA in the cancerous tissues were significantly lower than those in normal tissues (0.52 vs. 1.28, P < 0.01; 0.51 vs. 1.21, P < 0.01). Difference between the 2 mRNA splices was not significant in the matched tissues (P > 0.05). (2) The average ratios of light density of p33/ING(1) mRNA, and p47/ING(1) mRNA in the cancerous tissues of Dukes' C, D stage were significantly lower than those in cancerous tissues of Dukes' A, B stage (0.38 vs. 0.65, P < 0.01; 0.40 vs. 0.63, P < 0.01). (3) Of the 46 cases, no mutation of ING(1) gene was detected, only 5 (10.9%)cases of LOH were found. CONCLUSIONS: Abnormal alteration of ING(1) gene is rare in sporadic colorectal cancer. Its down-regulation may happen in transcription or post-transcription, and correlates tightly with the occurrence and progression of sporadic colorectal cancer.
