ABSTRACT
Cell adhesion is a crucial issue for cytotoxicity or anticancer effectiveness for tumor cells. However, how both nanoparticles and drugs affect cell adhesion has not yet been defined. Herein, we report for the first time that gold nanoparticles and Paclitaxel can disrupt adhesion, as well as enhance apoptosis of HepG2 cell individually and synergistically, as observed by in situ measurement using quartz crystal microbalance (QCM). It was also found by MTT assay that gold nanoparticles of low cellular cytotoxicity enhance the antiproliferation and apoptosis of HepG2 cell induced by Paclitaxel. Those findings would be of great potential for biomedical application of nanoparticles.
Subject(s)
Cell Adhesion/drug effects , Gold/pharmacology , Metal Nanoparticles , Paclitaxel/pharmacology , Antineoplastic Agents, Phytogenic/pharmacology , Apoptosis/drug effects , Biosensing Techniques/methods , Cell Line, Tumor , Cell Proliferation/drug effects , Humans , Microscopy, Electron, Scanning , QuartzABSTRACT
Based on the assumed flat model, Navier-Stokes equation for two-dimensional laminar flow fluid and the theory for diffusion and mass transfer, we obtained theoretical results with regarding to the concentration distribution in separation process by mass transfer model in the ultrafiltration process. Through theoretical analysis and experimental verification of concentration distribution, it was known that this model could not only preferably describe the concentration distribution of ultrafiltration process but also forecast concentration polarization phenomenon.
