ABSTRACT
OBJECTIVE: To investigate the influences of ultrafiltration and alcohol sedimentation on protective effects of Radix Astragali and Radix Hedyseri against rat's cerebral ischemia. METHODS: Using dexamethasone (im.) and ligating common carotid artery, the rat stasis model combined transient cerebral ischemia was established to evaluate the effects of the ultrafiltration and alcohol sedimentation through detecting antioxidant system and other indexes in brain tissue. RESULTS: The results showed that the 6 g/kg water extract(crude drug), ultrafiltration and alcohol sedimentation of Radix Astragali and Radix Hedyseri could upgrade adenosine-triphosphate (ATP), superoxide dismutase (SOD) and catalase (CAT), and degrade malondialdehyde(MDA) and water content of brain tissue in rat stasis model combined transient cerebral ischemia, the water extract and ultrafiltration of them could degrade lactic acid (LD) of brain tissue, and the effects of alcohol sedimentation of Radix Astragali and Radix Hedyseri become weaker than water extract of them. CONCLUSION: The water extract, ultrafiltration and alcohol sedimentation of Radix Astragali and Radix Hedyseri have some protective effects on cerebral ischemia in rats, the effective differences of the extract through the same extraction method are not remarkable, and alcohol precipitation method has obvious influences effect on Radix Astragali and Radix Hedyseri.
Subject(s)
Astragalus Plant/chemistry , Cerebral Infarction/drug therapy , Drugs, Chinese Herbal/pharmacology , Alcohols/chemistry , Animals , Antioxidants/metabolism , Astragalus propinquus , Brain/drug effects , Catalase/metabolism , Malondialdehyde/metabolism , Plant Roots/chemistry , Rats , Superoxide Dismutase/metabolism , UltrafiltrationABSTRACT
OBJECTIVE: To optimize the preparation process of beta-cyclodextrin (beta-CD) inclusion compound of Angelica oil. METHODS: Single factor experiment and orthogonal design were used together, oil inclusion rate was used as an index, then chose the best preparation technology. RESULTS: The optimal method was saturated solution method. The preparation condition for inclusion was as follows: the ratio of Angelica oil to beta-CD was (1: 8), the ratio of beta-CD to water was (1: 6) and the stirring time was 4 h. CONCLUSION: The preparation procedure of inclusion volatile oil is simple, stable, reliable and high inclusion rate.
Subject(s)
Angelica sinensis/chemistry , Oils, Volatile/chemistry , Plants, Medicinal/chemistry , Technology, Pharmaceutical/methods , beta-Cyclodextrins/chemistry , Analysis of Variance , Drug Carriers , Drug Stability , Drugs, Chinese Herbal/chemistry , Oils, Volatile/analysis , Oils, Volatile/isolation & purification , Plant Oils/analysis , Plant Oils/chemistry , Plant Oils/isolation & purification , Plant Roots/chemistry , Time Factors , Water/chemistryABSTRACT
OBJECTIVE: To choose the optimum initial processing methods of Rheum palmatum. METHODS: Fresh crude Rheum palmatum was sliced and dealt with the different drying methods such as sun drying, shady drying, microwave heating and various temperatures drying. The content of the Anthraquinones derivatives, slicing colors and dried rates were used as evaluation indexes. The sliced Rheum palmatum was compared with the traditional processing. RESULTS: Sliced fresh crude Rheum palmatum had the low content of the Anthraquinones derivatives and dry rates, slicing colours had obviously changes. For various drying methods, smoking drying was superior to sun drying, shady drying, microwave heating and various temperatures drying methods. CONCLUSION: Fresh crude Rheum palmatum is not suitable for slicing processing. The best drying method is smoking drying.
