ABSTRACT
This study aims to investigate the mechanism of tumor-derived exosomal (EVs) SNHG16 in promoting the progression of nasopharyngeal carcinoma (NPC). QRT-PCR was used to detect the expression of SNHG16, miR-23b-5p and MCM6 in NPC. MTT, flow cytometry and transwell were used to detect the effects of them on the proliferation, cycle, apoptosis and invasion ability of NPC. Transmission electron microscopy, Western blotting and BCA were used to verify the regulation of exosome secretion under different oxygen environments. Our results showed that hypoxia induces tumor-derived exosome SNHG16 to mediate NPC progression through the miR-23b-5p/MCM6 pathway.
Subject(s)
Exosomes , MicroRNAs , Nasopharyngeal Neoplasms , RNA, Long Noncoding , Humans , Nasopharyngeal Carcinoma/genetics , Nasopharyngeal Carcinoma/pathology , MicroRNAs/genetics , MicroRNAs/metabolism , Exosomes/genetics , Exosomes/metabolism , Hypoxia/genetics , Cell Proliferation/genetics , RNA, Long Noncoding/genetics , RNA, Long Noncoding/metabolism , Nasopharyngeal Neoplasms/genetics , Nasopharyngeal Neoplasms/pathology , Cell Line, Tumor , Minichromosome Maintenance Complex Component 6ABSTRACT
OBJECTIVE: Aimed to observe and analyse the diagnostic and therapeutic methods of the isolated sphenoid sinus disease, achieve earlier diagnosis and timelier intervention for this easily neglected disease and decrease the occurrence of misdiagnoses. METHOD: A retrospective study was conducted in 159 patients with isolated sphenoid sinus disease. RESULT: Headache was the most common presenting symptom (79.87%,127/159). Among the 159 cases, 60 (37.74%) had mucocele, 44 (27.67%) isolated sphenoiditis, 31 (19.50%) fungal sinusitis, 5 (3.14%) polyp, 3 (1.89%) fibrous dysplasia, 2 (1.26%) inverted papilloma, 3 (1.89%) chordoma, 3 (1.89%) squamous carcinoma, 3 (1.89%) malignant lymphoma, 2 (1.26%) neuroendocrine carcinoma, 2 (1.26%) olfactory neuroblastoma, and 1 (0.63%) malignant fibrohistiocytoma. A follow-up of 10 months to 4 years post-surgery showed good prognosis in most of the patients who underwent surgical therapy. CONCLUSION: The sphenoid sinus disease is often vague and nonspecific in its clinical presentation. The most common clinical symptom is headache, followed by vision changes. Endoscopic sphenoidotomy is the primary therapy for isolated sphenoid sinus disease.
