ABSTRACT
As a non-invasive method, low-intensity pulsed ultrasound (LIPUS) can accelerate fracture healing. The mechanisms responsible for the enhanced fracture healing need to be studied further. Activation of YAP/TAZ, key mediators of the Hippo signaling pathway, could promote angiogenesis and vascular remodeling. The purpose of this study was to determine whether LIPUS treatment can activate YAP/TAZ. Human umbilical vein endothelial cells (HUVEC) were used. After LIPUS treatment, Western blot and immunofluorescence staining were used for YAP/TAZ activation. Small interfering RNA (siRNA) of YAP and short hairpin LATS1/2 (shLATS1/2) were used to check whether there is cross-talk with the Hippo pathway. The phosphorylated YAP (p-127 and p-397) protein increased more than 3-fold 0.5 h after LIPUS treatment (p < 0.05). TAZ protein increased 3.0-, 2.0- and 1.5-fold 0.5, 6 and 12 h after LIPUS treatment. We found that LIPUS treatment activates YAP/TAZ, which is translocated into the cell nucleus to activate target genes. This process can be inactivated by siYAP and activated by shLATS1/2. The cross-talk with the Hippo pathway can initiate angiogenesis so as to accelerate fracture healing by LIPUS.
Subject(s)
Adaptor Proteins, Signal Transducing/metabolism , Human Umbilical Vein Endothelial Cells/metabolism , Neovascularization, Physiologic/physiology , Phosphoproteins/metabolism , Transcription Factors/metabolism , Ultrasonic Therapy/methods , Ultrasonic Waves , Adaptor Proteins, Signal Transducing/genetics , Blotting, Western , Cell Proliferation , Cells, Cultured , Fluorescent Antibody Technique , Humans , Intracellular Signaling Peptides and Proteins/metabolism , Phosphoproteins/genetics , Real-Time Polymerase Chain Reaction , Signal Transduction/physiology , Trans-Activators , Transcription Factors/genetics , Transcriptional Coactivator with PDZ-Binding Motif Proteins , YAP-Signaling ProteinsABSTRACT
PURPOSE: To evaluate the clinical effect of apicectomy and root canal obturation completed one time under direct vision on chronic periapical periodontitis of anterior teeth. METHODS: 34 cases(40 teeth) with RCT failing or larger periodontal lesion, which couldn't be cured depending on RCT only, were chosen for this clinical study. They were randomly divided into two groups with 20 teeth in each group. Root canal obturation was done after apicectomy under direct vision(group A),or apicectomy was done after RCT(group B).The time of filling process, the ratio of postobturation pain, the obturation quality and short-term efficiency of the treatment were assessed. The data were separately subjected to t test, Chi-square test, Mann-Whitney U test with SPSS12.0 software package. RESULTS: The time of filling process in group A was significantly less than group B (P<0.05); No statistically significant difference was found between group A and group B in the ratio of postobturation pain, obturation quality and clinical therapeutic efficiency. CONCLUSIONS: The method of apicectomy and root canal obturation completed one time under direct vision is rapid, simple and effective in treating chronic periapical periodontitis of anterior teeth.The short-term clinical therapeutic efficiency is similar to that of apicectomy done after RCT.
