ABSTRACT
This paper proposed a compact and portable planar waveguide evanescent wave immunosensor (EWI) for highly sensitive detection of BPA. The incident light is coupled into the planar waveguide chip via a beveled angle through undergoing total internal reflection, where the evanescent wave field forms and excites the binding fluorophore-tagged antibodies on the chip surface. Typical calibration curves obtained for BPA has detection limits of 0.03 µg/L. Linear response for BPA ranged from 0.124 µg/L-9.60 µg/L with 50% inhibition concentration for BPA of 1.09 ± 0.25 µg/L. The regeneration of the planar optical waveguide chip allows the performance of more than 300 assay cycles within an analysis time of about 20 min for each assay cycle. By application of effective pretreatment procedure, the recoveries of BPA in real water samples gave values from 88.3% ± 8.5% to 103.7% ± 3.5%, confirming its application potential in the measurement of BPA in reality.
Subject(s)
Benzhydryl Compounds/chemistry , Biosensing Techniques/methods , Immunoassay/methods , Phenols/chemistry , Water/analysis , Water/chemistry , Antibodies/chemistry , Calibration , Sensitivity and SpecificityABSTRACT
The fundus lesions appear in early pathological myopia and develops step by step,causing significant visual impairment.The occurrence of choroidal neovascularization is the main cause for the vision loss.This paper gives an introduction to the prevalence,damages,outcomes,affecting factors and treatment for pathologic myopia,and lays emphasis on the progress of medical treatment with anti-vascular endothelium growth factor.
