Complex Treatment and Prosthetic Rehabilitation of an Acquired Maxillofacial Defect with Tumor Invasion: Brief Report.

BACKGROUND: A patient was evaluated with respect to the effects and results of a complex treatment plan for complete dental rehabilitation. Several steps were required. Each step included immunological tests of salivary biomarkers. Clinical and immunological assessments were evaluated on Day 3, Week 2, Month 3, and Month 6 post-surgery. These evaluations guided the decision-making process with regard to preparation of a permanent prosthesis. OBJECTIVE: The aim of the study is to evaluate the response of tissues and organs of the maxillofacial region in patients during dental rehabilitation after maxillofacial surgery. METHODS: Complex treatment and rehabilitation involving cooperation between the specialists in maxillofacial surgery, prosthetic dentistry, and cancer immunology. RESULTS: Long-term monitoring and clinical examination showed a direct relationship between the patient's clinical and dental status and the changes in oral fluid biomarkers. CONCLUSION: The data revealed that the oral fluid biomarkers reflected the patient's adaptation to prosthodontic rehabilitation. Treatment and monitoring of a maxillofacial tumor patient could use biomarkers as a non-invasive indicator.

Enhancer of zeste homolog 2 depletion induces cellular senescence via histone demethylation along the INK4/ARF locus.

Polycomb group proteins are epigenetic transcriptional repressors that function through recognition and modification of histone methylation and chromatin structure. As a member of PcG proteins, enhancer of zeste homolog 2 (EZH2) targets cell cycle regulatory proteins which govern cell cycle progression and cellular senescence. In previous work, we reported that EZH2 depletion functionally induced cellular senescence in human gastric cancer cells with mutant p53. However, whether EZH2 expression contributes to the change of key cell cycle regulators and the mechanism involved are still unclear. To address this issue, we investigated the effects of EZH2 depletion on alteration of histone methylation pattern. In gastric cancer cells, INK4/ARF locus was activated to certain extent in consequence of a decrease of H3K27me3 along it caused by EZH2 silence, which contributed substantially to an increase in the expression of p15(INK4b), p14(ARF) and p16(INK4a) and resulted in cellular senescence ultimately. Furthermore, MKN28 cells, which did not express p16(INK4a) and p21(cip), could be induced to senescence via p15(INK4b) activation and suppression of p15(INK4b) reversed senescence progression induced by EZH2 downregulated. These data unravel a crucial role of EZH2 in the regulation of INK4/ARF expression and senescence procedure in gastric cancer cells, and show that the cellular senescence could just depend on the activation of p15(INK4b)/Rb pathway, suggesting the cell-type and species specificity involved in the mechanisms of senescence inducement.

THE EXTRA-ORGANIC RENAL ARTERIES / 解剖学报

1.The number of origin,points of entrance,and relationship with other structures of the renal arteries were studied in 111 adult male and 9 female cadavers. It was found that 84.2?2.35% belonged to 1 branch-type,of which 73.4?4.03% occurred bilaterally;the two-branch type consisted only 15.4?2.33%;the three branch type being only 0.4?0.41%. 2.The average height of origin of the renal arteries from the aorta was at the level of intervertebral disc between the 1st and 2nd lumbar vertebrae,of which 50.0?5.23% were equal in height on both sides;in 34.1?5.05% the right side was higher than the left and in 15.9?3.90% was the reverse. 3.When two renal arteries were present,the accessory one arose at a lower level than the main artery,ranging from the level of lower border of the 1st lumbar vertebra to that of the upper border of the 5th lumbar vertebra. 4.In 78.2?2.90% of the cases,the renal arteries entered the kidney through the hilum,the rest of them entered dinectly into the kidney substance,most of which through the upper extremity of the kidney. 5.When two renal arteries were present,both entered the hilum in 75.0?7.21% of the cases. 6.In most of the cases (83.7?2.60%),the renal artery divided outside of the hilum.Its primary branches could be divided into 5 types,most of which were of the anterior-posterior or superior-inferior types.They were 59.1?3.88 and 34.3?3.65% respectively. 7.The relationship of the accessory renal artery with the ureter and the inferior venal cava was discussed.