Metabolic cost of thermoregulation decreases after the molt in developing Weddell seal pups.

Allocation of energy to thermoregulation greatly contributes to the metabolic cost of endothermy, especially in extreme ambient conditions. Weddell seal (Leptonychotes weddellii) pups born in Antarctica must survive both on ice and in water, two environments with very different thermal conductivities. This disparity likely requires pups to allocate additional energy toward thermoregulation rather than growth or development of swimming capabilities required for independent foraging. We measured longitudinal changes in resting metabolic rate (RMR) for Weddell seal pups (n=8) in air and water from one to seven weeks of age, using open-flow respirometry. Concurrently, we collected molt, morphometric and dive behavior data. Absolute metabolic rate (MR) in air followed the expected allometric relationship with mass. Absolute MR in water was not allometric with mass, despite a 3-fold increase in mass between one and seven weeks of age. Developmental stage (or molting stage), rather than calendar age, determined when pups were thermally capable of being in the water. We consistently observed post-molt pups had lower RMR in air and water (6.67±1.4 and 7.90±2.38 ml O2 min-1 kg-1, respectively) than pre-molt (air: 9.37±2.42 ml O2 min-1 kg-1, water: 13.40±3.46 ml O2 min-1 kg-1) and molting pups (air: 8.45±2.05 ml O2 min-1 kg-1, water: 10.4±1.63 ml O2 min-1 kg-1). RMR in air and water were equivalent only for post-molt pups. Despite the increased energy cost, molting pups spent three times longer in the water than other pups. These results support the idea of an energetic trade-off during early development; pups expend more energy for thermoregulation in water, yet gain experience needed for independence.

Pinniped Ontogeny as a Window into the Comparative Physiology and Genomics of Hypoxia Tolerance.

Diving physiology has received considerable scientific attention as it is a central element of the extreme phenotype of marine mammals. Many scientific discoveries have illuminated physiological mechanisms supporting diving, such as massive, internally bound oxygen stores and dramatic cardiovascular regulation. However, the cellular and molecular mechanisms that support the diving phenotype remain mostly unexplored as logistic and legal restrictions limit the extent of scientific manipulation possible. With next-generation sequencing (NGS) tools becoming more widespread and cost-effective, there are new opportunities to explore the diving phenotype. Genomic investigations come with their own challenges, particularly those including cross-species comparisons. Studying the regulatory pathways that underlie diving mammal ontogeny could provide a window into the comparative physiology of hypoxia tolerance. Specifically, in pinnipeds, which shift from terrestrial pups to elite diving adults, there is potential to characterize the transcriptional, epigenetic, and posttranslational differences between contrasting phenotypes while leveraging a common genome. Here we review the current literature detailing the maturation of the diving phenotype in pinnipeds, which has primarily been explored via biomarkers of metabolic capability including antioxidants, muscle fiber typing, and key aerobic and anaerobic metabolic enzymes. We also discuss how NGS tools have been leveraged to study phenotypic shifts within species through ontogeny, and how this approach may be applied to investigate the biochemical and physiological mechanisms that develop as pups become elite diving adults. We conclude with a specific example of the Antarctic Weddell seal by overlapping protein biomarkers with gene regulatory microRNA datasets.

From ice to ocean: changes in the thermal function of harp seal pelt with ontogeny.

Many animals exhibit ontogenetic changes associated with adaptations for survival. Harp seals (Pagophilus groenlandicus) live in the Arctic and rely on thick insulation to maintain thermal homeostasis. Adult harp seals primarily use blubber for insulation, but newborn harp seals rely on a lanugo pelt while nursing, as their blubber layer develops and their first-year pelage grows. This study compared ontogenetic changes in the thermal properties of harp seal pelts in water and in air. Thermal conductivity, pelt thickness, and thermal resistance were measured in water for pelts of harp seal neonates (1 day old), thin whitecoats (4 day old), fat whitecoats (9 day old), ragged jackets (2 week old), beaters (3 week old), and adults and compared to previously published measurements made on the same pelts in air. Pelt conductivity was significantly higher in water than air for pre-molt and molting pups (P ≤ 0.031). Unlike adult pelage, which flattened underwater, lanugo hairs lifted underwater, a phenomenon that has not been reported previously. Thermal resistance of the pelt was significantly reduced in water compared to air for neonates and thin whitecoats (P ≤ 0.0001). A mathematical model of conductive heat transfer for an ellipsoid body showed volume-specific heat loss in water decreased and then stabilized as harp seals aged (P = 0.0321) and was significantly higher for neonates, thin whitecoats, and ragged jackets in water than in air (P ≤ 0.0089). Overall, pelt function is reduced in water for harp seal pups with lanugo, and this renders neonates and thin whitecoats particularly vulnerable to heat loss if submerged.