ABSTRACT
Large zeolite crystals of ferrierite have been used to study the deactivation, at the single particle level, of the alkyl isomerisation catalysis of oleic acid and elaidic acid by a combination of visible micro-spectroscopy and fluorescence microscopy (both polarised wide-field and confocal modes). The large crystals did show the desired activity, albeit only traces of the isomerisation product were obtained and low conversions were achieved compared to commercial ferrierite powders. This limited activity is in line with their lower external non-basal surface area, supporting the hypothesis of pore mouth catalysis. Further evidence for the latter comes from visible micro-spectroscopy, which shows that the accumulation of aromatic species is limited to the crystal edges, while fluorescence microscopy strongly suggests the presence of polyenylic carbocations. Light polarisation associated with the spatial resolution of fluorescence microscopy reveals that these carbonaceous deposits are aligned only in the larger 10-MR channels of ferrierite at all crystal edges. The reaction is hence further limited to these specific pore mouths.
Subject(s)
Mouth/chemistry , Oleic Acid/chemistry , Zeolites/chemistry , Catalysis , Isomerism , Microscopy, Fluorescence , Oleic Acid/chemical synthesisABSTRACT
The synthesis of a novel potent cyclic peptide MC4-ligand by ring-closing metathesis (RCM) is described. Based on the Ac-Nle-Gly-Lys-D-Phe-Arg-Trp-Gly-NH2-MC4 ligand, Ac-Nle-Alg-Lys-D-Phe-Arg-Trp-Alg-NH2 was designed and synthesized followed by cyclization using RCM. Both compounds are high affinity and selective MC4-R-agonists. The cyclic RCM-peptide was more potent in a rat-grooming assay.
Subject(s)
Peptides, Cyclic/chemistry , Peptides, Cyclic/metabolism , Receptor, Melanocortin, Type 4/metabolism , Animals , Cells, Cultured , Cyclization , Humans , Kidney/drug effects , Ligands , Melanocyte-Stimulating Hormones/chemistry , Melanocyte-Stimulating Hormones/metabolism , Rats , Structure-Activity RelationshipABSTRACT
The synthesis of potential beta-turn mimetics based on cyclic sulfonamide peptoid/peptoid hybrids is described. These are readily synthesized using a solid phase protocol followed by cyclization in solution, and their suitability to combinatorial approaches is illustrated by the synthesis of a small but diversely functionalized library.
Subject(s)
Peptides, Cyclic/chemical synthesis , Receptor, Melanocortin, Type 4/metabolism , Cyclization , Models, Chemical , Peptides, Cyclic/pharmacology , Receptor, Melanocortin, Type 4/agonists , Receptor, Melanocortin, Type 4/antagonists & inhibitors , Sulfonamides/chemical synthesis , Sulfonamides/pharmacologyABSTRACT
[reaction: see text] The solid-supported synthesis followed by cyclization in solution of cyclic (alpha(2)beta)-tripeptides, potential peptide beta-turn mimetics, is described. The cyclization takes advantage of facilitating the rotation between trans- and cis-rotamers of two amide bonds. The method is amenable to combinatorial approaches as is illustrated by the synthesis of a small array of cyclic (alpha(2)beta)-tripeptides.
