ABSTRACT
The functional significance of the proteasome activator REGγ in the regulation of cell proliferation and apoptosis has been recognized. However, pathological contributions to tumor development remain to be elucidated. Both oncogenic proteins and tumor suppressors are targeted by REGγ for proteasomal degradation. It has been proposed that the role of the REGγ in the pathogenesis of cancer is cell- and context-specific. In this study, we aimed to explore the potential involvement of REGγ in laryngeal carcinomas, comparing protein expression in tumor and adjacent tissues by immunohistochemical staining and Western blot analysis. We also characterized the correlation between the expression of REGγ and the previously identified substrates p53 and p21. We showed that REGγ was abnormally highly expressed in cancer tissues. Statistical analysis revealed that there was a positive relationship between the level of REGγ and the expression of p53 and p21. Our study suggests that REγ overexpression can facilitate the growth of laryngeal cancer cells.
Subject(s)
Autoantigens/biosynthesis , Cyclin-Dependent Kinase Inhibitor p21/biosynthesis , Laryngeal Neoplasms/metabolism , Proteasome Endopeptidase Complex/biosynthesis , Tumor Suppressor Protein p53/biosynthesis , Aged , Apoptosis , Autoantigens/metabolism , Cell Proliferation , Humans , Male , Middle Aged , Proteasome Endopeptidase Complex/metabolism , Tumor Suppressor Proteins/metabolismABSTRACT
OBJECTIVE: To identify the predisposing factors of deep neck abscess and review diagnosis and treatment experience. METHODS: A respective review was conducted in 50 cases who were diagnosed as having deep neck abscess in this hospital from Jan. 1997 to Dec. 2002. RESULTS: The causes of deep neck abscess were tooth diseases (3 cases), acute tonsillitis and laryngitis (8 cases), infection of upper respiratory tract (9 cases), foreign bodies in esophagus (14 cases), diabetes mellitus (5 cases), uncertain cause (11 cases). Among 21 cases of pus bacterial cultivation, 13 (64%) cases were positive. By different ways of drainage including neck-mediastinum incision, and use of large dosage of antibiotics, 46 six cases were cured and 4 cases died. Two died of massive hemorrhage of neck blood vessel burst, one massive hemorrhage of upper digestive tract, another infective shock. CONCLUSIONS: Once deep neck abscess is diagnosed, early surgical drainage, appropriate use of antibiotics and control of complications and accompanying diseases are very important to improve the cure rate.
