ABSTRACT
<p><b>OBJECTIVE</b>To investigate the distribution of GAD67 and the co-localization with bNOS in the main olfactory bulb of GAD67-GFP knock-in mouse.</p><p><b>METHODS</b>Polymerase chain reaction was applied to identify the genotype of GAD67-GFP knock-in mouse, the animals were sacrificed and frozen sections of olfactory bulb were prepared. The Nissl-staining was performed to show an framework of the neuron in the olfactory bulb. The distribution of GAD67 and co-localization with bNOS were detected by immunofluorescence technique.</p><p><b>RESULTS</b>The proportion of GAD67-positive cells among DAPI-positive cells were (42.98 ± 0.92)% in glomerular layer, (23.64 ± 0.84)% in mitral cell layer and (77.75 ± 0.84)% in granule cell layer; the bNOS-positive cells mainly existed in glomerular layer and mitral cell layer, very few in granule cell layer. No co-localization of GAD67 and bNOS in granule cell layer and mitral cell layer was found, but there was dispersed distribution in glomerular layer.</p><p><b>CONCLUSION</b>GAD67-positive neurons mainly appear in glomerular layer and granule cell layer, and the bNOS is mostly expressed in glomerular layer and mitral cell layer; while the co-localization of GAD67 and bNOS only occurs in glomerular layer of olfactory bulb.</p>
Subject(s)
Animals , Mice , Gene Knock-In Techniques , Glutamate Decarboxylase , Genetics , Metabolism , Green Fluorescent Proteins , Genetics , Metabolism , Mice, Transgenic , Neurons , Metabolism , Nitric Oxide Synthase Type I , Metabolism , Olfactory Bulb , Metabolism , Tissue DistributionABSTRACT
A novel upconversion luminescence agent 40CdF2 x 60BaF2 x 1.6Er2O3 was synthesized and the fluorescent spectrum was determined. This upconversion luminescence agent can emit five upconversion fluorescent peaks whose wavelengths are all below 387nm under the excitation of 488 nm visible light. This upconversion luminescence agent was mixed into nanometer TiO2 powder by ultrasonic dispersion and the doped nanometer TiO2 photocatalyst utilizing visible light was prepared. The doped TiO2 powder was charactered by XRD and TEM and its photocatalytic activity was checked through the photocatalytic degradation of ethyl violet dye as a model compound under the visible light irradiation emitted by three basic color lamp. Otherwise, in order to compare the photocatalytic activities the same experiment was carried out for undoped photocatalytic TiO2 powder. The degradation ratio of ethyl violet dye in the presence of doped nanometer TiO2 powder reached 99.68% under visible light irradiation at 12.0 h which was obviously higher than the corresponding degradation ratio in the presence of undoped nanometer TiO2 powder, which indicate that the upconversion luminescence agent prepared as dopant can effectively turn visible lights to ultraviolet lights which are absorbed by nanometer TiO2 particles and produce the electron-cavity pairs.
Subject(s)
Luminescent Agents/chemistry , Metal Nanoparticles/chemistry , Rosaniline Dyes/chemistry , Titanium/chemistry , Waste Disposal, Fluid/methods , Catalysis , Coloring Agents/chemistry , Light , Photochemistry , Photolysis/radiation effectsABSTRACT
A new upconversion luminescence agent, 40CdF2 x 60BaF2 x 0.8Er2O3, was synthesized and its fluorescent spectra were determined. This upconversion luminescence agent can emit five upconversion fluorescent peaks shown in the fluorescent spectra whose wavelengths are all below 387 nm under the excitation of 488 nm visible light. This upconversion luminescence agent was mixed into nano rutile TiO2 powder by ultrasonic and boiling dispersion and the novel doped nano TiO2 photocatalyst utilizing visible light was firstly prepared. The doped TiO2 powder was charactered by XRD and TEM and its photocatalytic activity was tested through the photocatalytic degradation of methyl orange as a model compound under the visible light irradiation emitted by six three basic color lamps. In order to compare the photocatalytic activities, the same experiment was carried out for undoped TiO2 powder. The degradation ratio of methyl orange in the presence of doped nano TiO2 powder reached 32.5% under visible light irradiation at 20 h which was obviously higher than the corresponding 1.64% in the presence of undoped nano TiO2 powder, which indicate the upconversion luminescence agent prepared as dopant can effectively turn visible lights to ultraviolet lights that are absorbed by nano TiO2 particles to produce the electron-cavity pairs. All the results show that the nano rutile TiO2 powder doped with upconversion luminescence agent is a promising photocatalyst using sunlight for treating the industry dye wastewater in great force.