The impact of microbial community structure changes on the migration and release of typical heavy metal (loid)s during the revegetation process of mercury-thallium mining waste slag.

The effect of changes in microbial community structure on the migration and release of toxic heavy metal (loid)s is often ignored in ecological restoration. Here, we investigated a multi-metal (mercury and thallium, Tl) mine waste slag. With particular focus on its strong acidity, poor nutrition, and high toxicity pollution characteristics, we added fish manure and carbonate to the slag as environmental-friendly amendments. On this basis, ryegrass, which is suitable for the remediation of metal waste dumps, was then planted for ecological restoration. We finally explored the influence of changes in microbial community structure on the release of Tl and As in the waste slag during vegetation reconstruction. The results show that the combination of fish manure and carbonate temporarily halted the release of Tl, but subsequently promoted the release of Tl and arsenic (As), which was closely related to changes in the microbial community structure in the waste slag after fish manure and carbonate addition. The main reason for these patterns was that in the early stage of the experiment, Bacillaceae inhibited the release of Tl by secreting extracellular polymeric substances; with increasing time, Actinobacteriota became the dominant bacterium, which promoted the migration and release of Tl by mycelial disintegration of minerals. In addition, the exogenously added organic matter acted as an electron transport medium for reducing microorganisms and thus helped to reduce nitrate or As (Ⅴ) in the substrate, which reduced the redox potential of the waste slag and promoted As release. At the same time, the phylum Firmicutes, including specific dissimilatory As-reducing bacteria that are capable of converting As into a more soluble form, further promoted the release of As. Our findings provide a theoretical basis for guiding the ecological restoration of relevant heavy-metal (loid) mine waste dumps.

Effects of microorganisms on the migration and transformation of typical heavy metal (loid)s in mercury-thallium mining waste slag during the combined application of fish manure and natural minerals.

Mercury-thallium mining waste slag has the characteristics of extremely acidic, low fertility and highly toxic polymetallic composite pollution, making it difficult to be treated. We use nitrogen- and phosphorus-rich natural organic matter (fish manure) and calcium- and phosphorus-rich natural minerals (carbonate and phosphate tailings) individually or in combination to amend the slag, analyze their effects on the migration and transformation of potentially toxic elements (Tl and As) in the waste slag. We set up sterile and non-sterile treatments specifically to further investigate the direct or indirect effect of microorganisms attached to added organic matter on Tl and As. The results showed that addition of fish manure and natural minerals to the non-sterile treatments promoted the release of As and Tl, resulting in an increase in As and Tl concentrations in the tailing lixiviums from 0.57 to 2.38-6.37 µg/L and from 69.92 to 107.51-157.21 µg/L, respectively. Sterile treatments promoted the release of As (from 0.28 to 49.88-104.18 µg/L) and inhibited the release of Tl (from 94.53 to 27.60-34.50 µg/L). Use of fish manure and natural minerals alone or in combination significantly reduced the biotoxicity of the mining waste slag, in which the combination was more efficient. XRD analysis showed that microorganisms in the medium promoted the dissolution of jarosite and other minerals, which indicated that the release and migration of As and Tl in Hg-Tl mining waste slag were closely related to microbial activities. Furthermore, metagenomic sequencing revealed that microorganisms such as Prevotella, Bacteroides, Geobacter, and Azospira, which were abundant in the non-sterile treatments, had remarkable resistance to a variety of highly toxic heavy metals and could affect the dissolution of minerals and the release and migration of heavy metals through redox reactions. Our results may aid in the rapid soilless ecological restoration of related large multi-metal waste slag dumps.

Influence mechanism of plant litter mediated reduction of iron and sulfur on migration of potentially toxic elements from mercury-thallium mine waste.

The decomposition of plant litter in soil changes soil nutrient content and plays an important role in regulating soil pH and availability of potentially toxic elements (PTEs). However, there remains limited studies on the mechanism under which litter influences the transport of PTEs in the process of ecological restoration. This study examined the effect of plant litter decomposition mediated reduction of iron and sulfur components on migration of PTEs from mercury-thallium mine waste. The results showed that the four kinds of litter alleviated the acidity of the waste, especially the Bpa and Tre litter. The nitro and nitroso groups produced by the decomposition of the litter were adsorbed onto the waste, thereby providing an electron transfer medium for iron reducing microorganisms, such as Geobacter. This promoted the reduction and release of Fe3+ to Fe2+ and reduced the electronegativity (El) value of waste. The reduced El promoted the adsorption of metal cations such as Hg and Tl to maintain electrical neutrality. However, it was not conducive to the adsorption of oxygen containing anions of As and Sb. An increase in litter resulted in an increase in reductivity of mercury-thallium mine waste. This maintained the reduction of Fe3+ to Fe2+ and changed or destroyed the structure of silicate minerals. PTEs, such as Tl, Hg, As, and Sb, were released, resulting in reductions in their residual fraction. However, the strong reduction conditions, especially the decomposition of Bpa, caused part of the released Hg(II) combining with S2- produced by the reduction of SO42- to form insoluble HgS, thereby reducing its migration. The findings could provide a theoretical basis to guide the situ-control and ecological restoration of PTEs in waste slag site.

Different composites inhibit Cd accumulation in grains under the rice-oilseed rape rotation mode of karst area: A field study.

Ensuring the safe production of food and oil crops in soils with elevated cadmium (Cd) content in karst regions is crucial. We tested a field experiment to examine the long-term remediation effects of compound microorganisms (CM), strong anion exchange adsorbent (SAX), processed oyster shell (POS), and composite humic acids (CHA) on Cd contamination in paddy fields under a rice-oilseed rape rotation system. In comparison to the control group (CK), the application of amendments significantly increased soil pH, cation exchange capacity (CEC), and soil organic matter (SOM) content while markedly decreasing the content of available Cd (ACd). During the rice cultivation season, Cd was predominantly concentrated in the roots. Relative to the control (CK), the Cd content in each organ was significantly reduced. The Cd content in brown rice decreased by 19.18-85.45%. The Cd content in brown rice following different treatments exhibited the order of CM > POS > CHA > SAX, which was lower than the Chinese Food Safety Standard (GB 2762-2017) (0.20 mg/kg). Intriguingly, during the oilseed rape cultivation season, we discovered that oilseed rape possesses potential phytoremediation capabilities, with Cd mainly accumulating in roots and stems. Notably, CHA treatment alone significantly decreased the Cd content in oilseed rape grains to 0.156 mg/kg. CHA treatment also maintained soil pH and SOM content, consistently reduced soil ACd content, and stabilized Cd content in RSF within the rice-oilseed rape rotation system. Importantly, CHA treatment not only enhances crop production but also has a low total cost (1255.230 US$/hm2). Our research demonstrated that CHA provides a consistent and stable remediation effect on Cd-contaminated rice fields within the crop rotation system, as evidenced by the analysis of Cd reduction efficiency, crop yield, soil environmental change, and total cost. These findings offer valuable guidance for sustainable soil utilization and safe production of grain and oil crops in the context of high Cd concentrations in karst mountainous regions.

Evaluation of Cadmium Transfer from Soil to the Human Body Through Maize Consumption in a Cadmium Anomaly Area of Southwestern China.

Evaluating the bioavailability, bioaccessibility, and transferability of cadmium (Cd) in soil-grain-human systems is essential in areas with a Cd anomaly in the karst region of southwestern China. In the present study, the main controlling factors and prediction models for Cd transfer in a soil-grain-human system were investigated in a typical area where natural processes and anthropogenic activities interact in the karst region of southwestern China. The environmental availability of Cd (diethylenetriaminepentaacetic acid- and CaCl2 -extractable Cd [ CdCaCl2 ]) in the soil varies significantly because of the diversity of soil properties. However, Cd concentrations in the maize grain were significantly related only to the CdCaCl2 concentrations in the soil (r = 0.595, p < 0.01), indicating that soil CdCaCl2 is a good indicator for evaluating Cd uptake by maize grain. Of all the measured soil properties, the soil cation exchange capacity (CEC) and the soil calcium (Casoil ) were the most important factors influencing Cd accumulation in the soil-maize grain system. A transfer model combining CdCaCl2 , soil CEC, and Casoil was sufficiently reliable for predicting Cd accumulation in the maize grain (R2 = 0.505). Although there is room for improvement regarding the prediction performance of the chain model combining soil CdCaCl2 with Casoil to predict the bioaccessible Cd concentration in maize grain (R2 = 0.344 for the gastric phase and R2 = 0.356 for the gastrointestinal phase), our findings provide a useful reference to further explore a model that can be used for a relatively rapid and reliable estimation of dietary Cd exposure for specific regions prior to crop harvest. Environ Toxicol Chem 2021;40:2923-2934. © 2021 SETAC.

Heavy metals accumulation and risk assessment in a soil-maize (Zea mays L.) system around a zinc-smelting area in southwest China.

Smelting of nonferrous metals causes significant concerns because of its emissions of heavy metals (HMs) into surface soil, and its potential threat to human health through the food chain. To investigate the HMs concentrations in a soil-maize system, a total of 41 paired soil-maize samples were collected from a typical indigenous zinc-smelting area of northwestern Guizhou Province, China. Results showed that the concentrations of the targeted HMs in the soil were significantly higher than their corresponding background values of Guizhou Province. Results obtained of the geo-accumulation index (Igeo) and the potential ecological risk factor of an individual metal (E r i ) revealed that Cd and Pb were identified as the top-priority control HMs in the study area. The mean concentrations in maize grain decreased in the order of Zn > Pb > Cu > Cd. Bio-accumulation factor (BAF) indicated a strong ability for Cd to be accumulated in the maize root. Translocation factor (TF) and Fourier transform infrared (FTIR) spectroscopy revealed that the maize root played an important role in reducing the mobilization of HMs to stem, except for Zn. Kriging interpolation results illustrated that the spatial distribution patterns of HMs in the maize grain were generally similar to those in sampled soil, and the higher concentrations for the investigated HMs were partially overlapping between soil and maize grain. The average hazard quotient (HQ) of the investigated HMs for both children and adults were all lower than the threshold value (HQ = 1). The total hazard index (HI) was 5.51E-01 and 4.24E-01 for the two population groups, respectively, implying no potential non-carcinogenic risk for local maize-consumers. Sensitivity analysis demonstrated that the oral ingestion rate (IngR) of grain was the predominated contribution to the output of the risk assessment.

Releasing Characteristics and Biological Toxicity of the Heavy Metals from Waste of Mercury-Thalliummine in Southwest Guizhou of China.

In this paper, the releasing characteristics and biological toxicity of Tl, Hg, As and Sb in waste of Lanmuchang mercury-thallium mine were studied. The results indicated that strong acidity can significantly promote the release of Tl from waste. With the increase of pH, the release of Sb grew steadily, while Hg and As showed a trend of first increasing and then decreasing. Fe2(SO4)3 contributed less to the release of As and Sb than to that of Hg and Tl. FeCl3 significantly inhibited the release of As, Sb and Tl. In the leaching experiments of litter and root exudates, the lixiviums appeared neutral, and the litter and root exudates solution significantly reduced the release of Tl, and showed less toxicity to luminescent bacteria. However, they promoted the release of Hg, As and Sb at different levels.

Migration characteristics of heavy metals in the weathering process of exposed argillaceous sandstone in a mercury-thallium mining area.

Lanmuchang mercury-thallium mine, a typical polymetallic mine is located in southwestern Guizhou, China, is the most serious and typical area resulted from multi-metal contamination (Tl, Hg, As, and Sb). After the mercury-thallium mining, a large area of surrounding rocks such as argillaceous sandstone with high contents of Tl, Hg, As, and Sb is exposed to air. Weathering caused the argillaceous sandstone to form different weathering layers, including the grey-black external layer, the brown-yellow middle layer and the gray-white inner layer, and the external layer was enriched with higher heavy metals. However, the reason of heavy metal migration and transformation in argillaceous sandstone caused by weathering is unclear. The objective of this paper was to investigate the migration, transformation and release characteristics of Tl, Hg, As, and Sb in argillaceous sandstone during the weathering. The results indicated that weathering not only promoted an acidic oxidation environment in argillaceous sandstone, but also increased its specific surface area, pore volume and hydrophilicity, which are beneficial to the permeability of oxygen and etching liquids during the process of weathering and leaching. Meanwhile, weathering led to the transformation or decomposition of hydrophilic groups, such as -OH and -CË­O in the grey-black external layer of argillaceous sandstone, resulting in the further release of heavy metals bound to these groups. The concentration of Tl, Hg, As, and Sb in the leaching solution of argillaceous sandstone represented a positive correlation with that of Fe, Ca, Mg at different levels, indicating that Tl, Hg, As, and Sb were released with the dissolution of Fe, Ca and Mg during weathering and leaching. In summary, these results indicated that weathering caused the dissolution and migration of heavy metals in the argillaceous sandstone. Tl, Hg, As, and Sb migrated from the grey-white inner layer to the grey-black external layer and partially adsorbed by free alumina (Ald), jarosite and Ca-bearing minerals, showing enrichment phenomena, partially released into the environment, causing environmental pollution.