ABSTRACT
Purpose To identify the binding of hypoxia in-ducible factor-2a (HIF-2 a) to the hypoxia-response element(HRE) of the matrixmetallo proteinases-2 (MMP-2) gene promoter region and clear the binding site. Methods Electro-phoretic mobility shift assay (EMSA) was used to identify the binding of HIF-2 a to HRE of the MMP-2 gene promoter region in vitro. At the same time, the chromatin immunoprecipitation assay (CHIP) was used to further determine the binding site. Results Successful prediction of two potential HIF-2a binding sites of MMP-2 the promoter region, which were-217～-204 and-1 029 ～-1 007, respectively. Probe test shows that the marked efficiency of sense chain and antisense chain was above 50％, and they could be used for EMSA-electrophoretic mobility shift assay. The results of EMSA showed that there was a binding site of HIF-2 a sense chain and antisense chain moter region int-217～-204. The results of chromatin immuno-precipitation showed that in the experimental group and control group an about 250 bp fragment in MMP-2 promoter containing HRE region was amplified, suggesting that the protein of HIF-2a binded to the HRE in MMP-2 promoter region in vivo. Conclusion HIF-2 a in MMP-2 promoter regionne promoter region in vitro and in vivo.
ABSTRACT
The asymmetric unit of the title compound, C(5)H(7)N(5) (2+)·2C(7)H(7)O(3)S(-)·H(2)O, consists of one diprotonated adeninium cation, two p-toluene-sulfonic acid anions and one water mol-ecule. In the crystal, the cations and anions are connected through N-Hâ¯O hydrogen bonds forming R(2) (2)(8) and R(2) (2)(9) graph-set motifs. The solvent water mol-ecule links cations and anions through O-Hâ¯O and N-Hâ¯O hydrogen bonds, generating a two-dimensional layer parallel to (10).
ABSTRACT
In the title compound, C(17)H(18)ClN(3)O(3), the dihedral angle between the planes of the two benzene rings is 1.03â (7)°. The overall conformation of the mol-ecule is influenced, in part, by electron delocalization and by an intra-molecular bifurcated O-Hâ¯(O,N) hydrogen bonds. The O atoms of the nitro group, one of which serves as an H bond acceptor, are disordered over two sets of sites with refined occupancies of 0.56â (3) and 0.44â (3).
ABSTRACT
In the title compound, C(11)H(13)N(2)O(+)·C(2)H(3)O(2) (-)·H(2)O, the dihedral angle between the benzene ring and the imidazole ring is 7.83â (6)°. In the crystal structure, N-Hâ¯O and O-Hâ¯O hydrogen bonds form a two-dimensional network. All the methyl H atoms are disorderd over two sites with equal occupancies.
ABSTRACT
In the title compound, C(16)H(9)BrO(5)·2C(3)H(7)NO, the chromene ring system is essentially planar. The two dimethyl-formamide solvent mol-ecules are linked by inter-molecular O-Hâ¯O hydrogen bonds to the 6-bromo-3-hydr-oxy-4-oxo-2-phenyl-4H-chromene-8-carboxylic acid molecules.
ABSTRACT
The europium(III) ion in the title compound, [Eu(2)(C(8)H(6)NO(2))(6)(C(12)H(8)N(2))(2)]·5H(2)O, is coordinated by seven carboxyl-ate O atoms and two N atoms from one phenanthroline mol-ecule. The carboxyl-ate groups of 3-(3-pyrid-yl)acrylate link pairs of europium(III) ions, forming centrosymmetric dinuclear units, which further assemble into a sheet parallel to the (001) plane through hydrogen-bonding inter-actions involving the uncoordinated water mol-ecules. One water molecule is disordered.
ABSTRACT
The title compound, C(7)H(4)Br(2)O(3), has an intra-molecular O-Hâ¯O=C hydrogen bond and aggregates to form hydrogen-bonded dimers via O-Hâ¯O inter-actions. The formation of zigzag one-dimensional mol-ecular tapes via C-Hâ¯Br inter-actions and π-π stacking inter-actions (inter-planar separation = 3.42â Å) completes the crystal structure.
