ABSTRACT
To study the chemical constituents in the non-alkaloid part of stems of Dendrobium nobile. The macroporous adsorption resin, MCI, silica gel, RP-C_(18), and Sephadex LH-20 gel, preparative thin layer chromatography, and preparative high-performance liquid chromatography(HPLC) were used to isolate and purify the compounds. The structures of the compound were determined according to the spectra data, physicochemical properties, and relevant references. A total of 8 compounds were isolated from D. nobile, which were soltorvum F(1), p-hydroxyphenylpropionic acid(2), vanillic acid(3), p-hydroxybenzoic acid(4), N-trans-cinnamic acid acyl-p-hydroxybenzene ethylamine(5),(+)-(1R,2S,3R,4S,5R,6S,9R)-2,11,12-trihydroxypicrotoxane-3(15)-lactone(6), dendronobilin H(7), soltorvum E(8). Compound 1 was a novel compound, named as soltorvum F. Compound 8 was isolated from Dendrobium species for the first time.
Subject(s)
Dendrobium/chemistry , Molecular Structure , Sesquiterpenes, Guaiane , Sesquiterpenes/chemistryABSTRACT
A novel method using a fiber optic-based fluorescence chemical sensor (FOCS) was developed for the preparation of on-line continuous monitoring of a drug in animals. First, an accurate optical design was used to enhance the intensity of light from a 100-microm optic fiber so the fluorescence signal can be detected. Second, A new sol-gel method was used to fix the fluorescence substance 4-(N,N-dioctyl)amino-7-nitrobenz-2-oxa-1,3-diazole (D-70) on the tip of the fiber. The variation in the quenching means variation in the concentration of Adriamycin (ADM) in rabbit's blood. ADM is determined by FOCS based on fluorescence multiple quenching. In a simple animal model, the carotid artery was catheterized with a cannula, housing a 100-microm optic fiber. The average recovery of all the tested compounds within the set concentration range was 99.4-106.2%; the within-day and between-day repeatability values were acceptable between 6.6-11.4% and 5.9-11.7%. The method permitted detection limits as low as 0.057 microg x mL(-1) at a signal-to-noise ratio of 3. The fiber optic chemical sensor is potentially useful for monitoring blood concentrations of drugs and metabilities in the biomedical field.
Subject(s)
Doxorubicin/blood , Drug Monitoring/methods , Animals , Aorta , Catheterization , Fiber Optic Technology , Fluorescence , Optical Fibers , Rabbits , Sensitivity and Specificity , Spectrometry, FluorescenceABSTRACT
<p><b>AIM</b>To evaluate the feasibility and accuracy of continuous monitoring of drug with a fiber optic chemical sensor (FOCS) in animal.</p><p><b>METHODS</b>An accurate optical design was used to enhance the intensity of light from a 100-micron optic fiber and the fluorescence signal could be detected. A new sol-gel method was used to fix the fluorescence substance 4-(N, N-dioctyl) amino-7-nitrobenz-2-oxa-1, 3-diazole (D-70) on the body fiber. The vary quenching means the vary of the concentration of adriamycin (ADM) in rabbit blood. ADM was determined by FOCS based on the fluorescence multiple quenching. In a simple animal model, the carotid artery was catheterized with a cannula, housing a 100-micron optic fiber.</p><p><b>RESULTS</b>The recovery of ADM was 99.4%-106.2%, the within-run and between-run RSDs were 6.6%-11.4% and 5.9%-11.7% respectively. The method permitted detection limits as low as 0.057 microgram.mL-1 at a signal-to-noise ratio of 3.</p><p><b>CONCLUSION</b>Fiber-optic chemical sensor is potentially useful for monitoring blood drug in biomedical field.</p>
