Expression of nerve growth factor and hypoxia inducible factor-1α and its correlation with angiogenesis in non-small cell lung cancer / 华中科技大学学报（医学）（英德文版）

In order to investigate the expression of nerve growth factor (NGF) and hypoxia inducible factor-1α (HIF-1α) and its correlation with angiogenesis in non-small cell lung cancer (NSCLC), paraffin-embedded tissue blocks from 20 patients with NSCLC were examined. Twenty corresponding para-cancerous lung tissue specimens were obtained to serve as a control. The expression of NGF, HIF-1α, and vascular endothelial growth factor (VEGF) in the NSCLC tissues was detected by using immunohistochemistry. The microvascular density (MVD) was determined by CD31 staining. The results showed that the expression levels of NGF, HIF-1α and VEGF in the NSCLC tissues were remarkably higher than those in the para-cancerous lung tissues (P<0.05). There was significant difference in the MVD between the NSCLC tissues (9.19±1.43) and para-cancerous lung tissues (2.23±1.19) (P<0.05). There were positive correlations between NGF and VEGF, between HIF-1α and VEGF, and between NGF and HIF-1α in NSCLC tissues, with the spearman correlation coefficient being 0.588, 0.519 and 0.588, respectively. In NSCLC tissues, the MVD had a positive correlation with the three factors (P<0.05). Theses results suggest that NGF and HIF-1α are synergically involved in the angiogenesis of NSCLC.

Expression of nerve growth factor and hypoxia inducible factor-1α and its correlation with angiogenesis in non-small cell lung cancer / 华中科技大学学报（医学）（英德文版）

In order to investigate the expression of nerve growth factor (NGF) and hypoxia inducible factor-1α (HIF-1α) and its correlation with angiogenesis in non-small cell lung cancer (NSCLC), paraffin-embedded tissue blocks from 20 patients with NSCLC were examined. Twenty corresponding para-cancerous lung tissue specimens were obtained to serve as a control. The expression of NGF, HIF-1α, and vascular endothelial growth factor (VEGF) in the NSCLC tissues was detected by using immunohistochemistry. The microvascular density (MVD) was determined by CD31 staining. The results showed that the expression levels of NGF, HIF-1α and VEGF in the NSCLC tissues were remarkably higher than those in the para-cancerous lung tissues (P<0.05). There was significant difference in the MVD between the NSCLC tissues (9.19±1.43) and para-cancerous lung tissues (2.23±1.19) (P<0.05). There were positive correlations between NGF and VEGF, between HIF-1α and VEGF, and between NGF and HIF-1α in NSCLC tissues, with the spearman correlation coefficient being 0.588, 0.519 and 0.588, respectively. In NSCLC tissues, the MVD had a positive correlation with the three factors (P<0.05). Theses results suggest that NGF and HIF-1α are synergically involved in the angiogenesis of NSCLC.

Representation and in vitro transdermal characteristics of glycyrrhizic acid nanoparticles prepared by antisolvent recrystallization method / 中草药

Objective: To prepare glycyrrhizic acid nanoparticles (GAN) using ethanol as solvent and ethyl acetate as the antisolvent, and to investigate the osmosis of GAN as carrier of dermal administration. Methods: Using scanning electron microscope (SEM), laser particle size analyzer, Fourier transform-infrared spectroscopy (FT-IR), and release rate analysis, the untreated glycyrrhizic acid (GA) and GA powder were characterized; In vitro cutaneous permeation experiments were carried out on modified Franz diffusion cells, using excised mouse skin. The concentration of diammonium glycyrrhizinate in the receptor compartments and skin were determined by HPLC. Results: The GAN was spherical, the average particle size was 220 nm, and the dissolution rate of nanosized glycyrrhizinate was improved obviously. The transdermal rate of nanosized glycyrrhizinate was better than the original glycyrrhizin, and the 12 h unit accumulation transmissibility of GAN and GA was 78.51 and 9.792 μg/cm2, respectively. Conclusion: After preparing, the particle size becomes smaller, the chemical structure does not change significantly, and both of dissolution rate and in vitro transdermal performance have been improved. The GAN has the potential application values in the development of pharmaceutical industry.

[Study on the chemical constituents of the essential oils from Chrysanthemum indicum collected in Jinyun Mountains, Chongqing].

OBJECTIVE: To analyse the chemical constituents of essential oils from Chrysanthemum indicum collected in Jinyun Mountains. METHODS: The essential oil was extracted by steam distillation and analyzed by GC-MS. RESULTS: The GC chromatogram of the essential oil had 92 peaks among which 69 were identified to be 66 compounds whose relative contents summed to account for 90. 07% of the total essential oil. CONCLUSION: It indicates Chrysanthemum indicum in Jinyun Mountains is good as medicine or for food. The place of origin has obvious influence on the chemical compostions of Chrysanthemum indicum's essential oil.

[Influence of processing methods on the chemical composition of the essential oil from Aucklandia lappa].

OBJECTIVE: To investigate the effects of different processing methods on chemical compositions of essential oil from Aucklandia lappa. METHODS: 4 different methods, frying without additional ingredients (1), stir-heating with bran (2), roasting surrounded with bran (3), and roasting wrapped in wet paper (4) were used to process raw Radix Aucklandiae and the contents and chemical compositions of essential oil were compared in the processed products with those in the raw drug. The essential oils were extracted wtih supercritical CO2 and analyzed by GC-MS. RESULTS: Except method 1, processing lowered the content of the essential oil in Radix Aucklandiae significantly. 29, 33, 32, 34, and 33 compounds were identified, respectively, in the essential oils from raw Radix Aucklandiae and the processed products of Radix Aucklandia with method 1, 2, 3, and 4. The chemical compositions of essential oil of Radix Aucklandiae changed a lot in processing,some components disappeared, such as alpha-phellandrene; Some came into being, such as alpha-ionone, alpha-caryophyllene, beta-sesquiphellandrene, alpha-longifolene,and so on, some increased in contents,such as elemene, dihydro-a-ionone, beta-caryophyllene, and so on. The contents of costunolide and dehydrocostuslactone in Radix Aucklandiae decreased in processing with method 2, 3, and 4. CONCLUSION: As a perfume meterial, Radix Aucklandiae should be processed by roasting surrounded with wheat bran, while as a drug to treat epigastric pain, the raw drug should be processed by stir-heating.