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1.
Acta Biochim Biophys Sin (Shanghai) ; 55(9): 1425-1433, 2023 Jul 31.
Article in English | MEDLINE | ID: mdl-37525533

ABSTRACT

Chondrocyte senescence is an important mechanism underlying osteoarthritis in the senile population and is characterized by reduced expressions of the extracellular matrix proteins. The involvement of glycolysis and the tricarboxylic acid cycle in the development of osteoarthritis is inclusive. The present study aims to investigate the role of the glycolytic enzyme M2 isoform of pyruvate kinase (PKM2) in chondrocytes in senescence and inflammation. Primary chondrocytes are isolated from the knee joints of neonatal mice. Small interfering RNAs (siRNAs) against PKM2 are transfected using lipofectamine. RNA sequencing is conducted in primary chondrocytes with the PKM2 gene deleted. Cell apoptosis, autophagy, reactive oxygen species measurement, and senescent conditions are examined. The glycolytic rate in cells is measured by Seahorse examination. Interleukin 1-ß (IL-1ß) increases the protein expressions of matrix metallopeptidases (MMP)13 and PKM2 and reduces the protein expression of collagen type II (COL2A1) in primary chondrocytes. Silencing of PKM2 alters the protein expressions of MMP13, PKM2, and COL2A1 in the same pattern in quiescent and stimulated chondrocytes. RNA sequencing analysis reveals that PKM2 silencing reduces senescent biomarker p16 INK4a expression. Compared with low-passage chondrocytes, high-passage chondrocytes exhibit increased expression of p16 INK4a and reduced expression of COL2A1. Silencing of PKM2 reduces SA-ß-Gal signals and increases COL2A1 expression in high-passage chondrocytes. Seahorse assay reveals that PKM2 deletion favors the tricarboxylic acid cycle in mitochondria in low- but not in high-passage chondrocytes. In summary, the glycolytic enzyme PMK2 modulates chondrocyte senescence but does not participate in the regulation of inflammation.


Subject(s)
Osteoarthritis , Animals , Mice , Cellular Senescence/genetics , Chondrocytes/metabolism , Cyclin-Dependent Kinase Inhibitor p16/genetics , Glycolysis , Inflammation/genetics , Inflammation/metabolism , Interleukin-1beta/metabolism , Osteoarthritis/genetics , Osteoarthritis/metabolism , RNA, Small Interfering/metabolism
2.
Mol Biol Evol ; 39(1)2022 01 07.
Article in English | MEDLINE | ID: mdl-34730826

ABSTRACT

Colonization of land from marine environments was a major transition for biological life on Earth, and intertidal adaptation was a key evolutionary event in the transition from marine- to land-based lifestyles. Multicellular intertidal red algae exhibit the earliest, systematic, and successful adaptation to intertidal environments, with Porphyra sensu lato (Bangiales, Rhodophyta) being a typical example. Here, a chromosome-level 49.67 Mb genome for Neoporphyra haitanensis comprising 9,496 gene loci is described based on metagenome-Hi-C-assisted whole-genome assembly, which allowed the isolation of epiphytic bacterial genome sequences from a seaweed genome for the first time. The compact, function-rich N. haitanensis genome revealed that ancestral lineages of red algae share common horizontal gene transfer events and close relationships with epiphytic bacterial populations. Specifically, the ancestor of N. haitanensis obtained unique lipoxygenase family genes from bacteria for complex chemical defense, carbonic anhydrases for survival in shell-borne conchocelis lifestyle stages, and numerous genes involved in stress tolerance. Combined proteomic, transcriptomic, and metabolomic analyses revealed complex regulation of rapid responses to intertidal dehydration/rehydration cycling within N. haitanensis. These adaptations include rapid regulation of its photosynthetic system, a readily available capacity to utilize ribosomal stores, increased methylation activity to rapidly synthesize proteins, and a strong anti-oxidation system to dissipate excess redox energy upon exposure to air. These novel insights into the unique adaptations of red algae to intertidal lifestyles inform our understanding of adaptations to intertidal ecosystems and the unique evolutionary steps required for intertidal colonization by biological life.


Subject(s)
Proteomics , Rhodophyta , Acclimatization/genetics , Adaptation, Physiological/genetics , Ecosystem , Rhodophyta/genetics
3.
Opt Lett ; 35(24): 4172-4, 2010 Dec 15.
Article in English | MEDLINE | ID: mdl-21165127

ABSTRACT

This investigation demonstrates leakage coupling between fundamental-mode and high-order mode resonance based on a periodical silica thin-film long-period grating (TFLPG) that was coated on a tapered fiber when wavelengths longer than the fundamental-mode cutoff were propagated. For the leaky guiding situation, these leakage modes still may exhibit strong mode coupling in the taper with the assistance of the TFLPG when the phase-matched condition is satisfied. An extremely high tuning efficiency of 62.9 nm/°C, which is equivalent to a measurement of sensitivity of approximately 168, 182 nm per refractive index unit, is achieved. To the best of our knowledge, this sensitivity is the highest achieved for a fiber sensor to date.

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