ABSTRACT
Plasmid-positive Yersinia bacteria of different species and different serotypes were analysed with respect to the immunological relationship of two of their plasmid-encoded outer membrane proteins (OMPs) by the immunoblot technique using three monoclonal antibodies (mAb), which were induced against OMPs of Yersinia enterocolitica serotype O:9 bacteria. Evidence is presented that these OMPs display discrete epitopes, which are common to all plasmid-positive Yersinia strains tested, with only one exception, indicating a close structural relationship of the OMPs analysed.
Subject(s)
Antibodies, Monoclonal , Bacterial Outer Membrane Proteins/immunology , Epitopes/analysis , Yersinia/immunology , Cross Reactions , PlasmidsABSTRACT
LPS-specific monoclonal antibodies induced against Y. enterocolitica serotype O:9 bacteria and against Y. enterocolitica O:3 were used in a comparative study to characterize the O:9 and O:3 LPS. Yersinia bacteria grown at 22 degrees C and 37 degrees C and LPS preparations thereof were tested. SDS-PAGE, Western blot analysis and adsorption procedures revealed that LPS of Y. enterocolitica O:9 differed from that of Y. enterocolitica O:3 in: (i) its repeating LPS O-side-chain sugar, (ii) its shorter length of the LPS O-side-chain and (iii) its failure to show temperature-dependent variation in the length of O-side-chains.
Subject(s)
Antibodies, Monoclonal/immunology , Antigens, Bacterial/immunology , Lipopolysaccharides/immunology , Yersinia enterocolitica/immunology , Animals , Dogs , Electrophoresis, Polyacrylamide Gel , Enzyme-Linked Immunosorbent Assay , Humans , ImmunoblottingABSTRACT
The plasmids of Yersinia enterocolitica and Yersinia pseudotuberculosis tested differed in regard to the following aspect: there were some of a molecular size of 41 to 47 Mdal which could be correlated with autoagglutination of the host bacteria, and the others with a smaller size of 19 to 34 Mdal that lacked the capacity to code for autoagglutination. Analysis of both types of plasmids with the restriction endonucleases Bam HI, Eco RI and Hind III besides of some similarities revealed also considerable serogroup-specific differences in their DNA fragmentation pattern.
Subject(s)
Plasmids , Yersinia enterocolitica/genetics , Yersinia pseudotuberculosis/genetics , DNA Restriction Enzymes , DNA, Bacterial/analysis , Electrophoresis, Agar GelABSTRACT
Human papillomavirus (HPV) 8 induces skin tumors which are at high risk for malignant conversion. The nucleotide sequence of HPV8 has been determined and compared to sequences of papillomaviruses with different oncogenic potential. The general organization of the HPV8 genome is similar to that of other types. Highly conserved, genus-specific sequences were found in open reading frames (ORFs) E1, E2, and L1. In ORFs E6, E7, and L2, HPV8 is more distantly related, but it was possible to differentiate subgenera in which HPV8 belonged to the HPV1-cottontail rabbit papillomavirus group. Sequences within ORF E4 and part of ORF L2 are rather type specific. HPV8 stands out by several unique features: the considerably reduced size of the noncoding region (397 base pairs), with a seemingly low potential for forming complex secondary structures; a cluster of putative promoter elements in the 3' half of ORF E1; an RNA polymerase III promoter-like sequence close to the C terminus of ORF E2; and of particular interest, the homology between the putative protein encoded by ORF E4 and the Epstein-Barr virus nuclear antigen 2 protein, which may reflect similar mechanisms in virus-mediated transformation.
