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1.
Exp Parasitol ; 184: 1-10, 2018 Jan.
Article in English | MEDLINE | ID: mdl-29162347

ABSTRACT

Trypanosoma cruzi, the etiologic agent of Chagas disease, has to cope with reactive oxygen and nitrogen species during its life cycle in order to ensure its survival and infection. The parasite detoxifies these species through a series of pathways centered on trypanothione that depend on glutathione or low molecular mass dithiol proteins such as tryparedoxins. These proteins transfer reducing equivalents to peroxidases, including mitochondrial and cytosolic peroxiredoxins, TcMPx and TcCPx, respectively. In T. cruzi two tryparedoxins have been identified, TXNI and TXNII with different intracellular locations. TXNI is a cytosolic protein while TXNII due to a C-terminal hydrophobic tail is anchored in the outer membrane of the mitochondrion, endoplasmic reticulum and glycosomes. TXNs have been suggested to be involved in a majority of biological processes ranging from redox mechanisms to protein translation. Herein, a comparison of the TXNII interactomes under physiological and oxidative stress conditions was examined. Under physiological conditions, apart from the proteins with unknown biological process annotation, the majority of the identified proteins are related to cell redox homeostasis and biosynthetic processes, while under oxidative stress conditions, are involved in stress response, cell redox homeostasis, arginine biosynthesis and microtubule based process. Interestingly, although TXNII interacts with both peroxiredoxins under physiological conditions, upon oxidative stress, TcMPx interaction prevails. The relevance of the interactions is discussed opening a new perspective of TXNII functions.


Subject(s)
Oxidative Stress/physiology , Peroxiredoxins/metabolism , Thioredoxins/metabolism , Trypanosoma cruzi/chemistry , Trypanosoma cruzi/physiology , Cell Membrane/metabolism , Cytosol/enzymology , Electrophoresis, Gel, Two-Dimensional , Electrophoresis, Polyacrylamide Gel , Fluorescent Antibody Technique, Indirect , Hydrogen Peroxide/pharmacology , Mitochondria/enzymology , Mitochondrial Membranes/metabolism , Permeability , Peroxidases/metabolism , Protozoan Proteins/metabolism , Transfection , Trypanosoma cruzi/enzymology
2.
Biochim Biophys Acta ; 1864(1): 1-10, 2016 Jan.
Article in English | MEDLINE | ID: mdl-26527457

ABSTRACT

Trypanosoma cruzi depends on the effectiveness of redox metabolism to survive and ensure infection in the host. Homeostasis of redox metabolism in T. cruzi is achieved by the actions of several proteins that differ in many aspects from host proteins. Although extensive research has been performed examining hydroperoxide cytosolic antioxidant defense centered on trypanothione, the mechanisms of mitochondrial antioxidant defense are not yet known. The aim of this study was to elucidate the partners of TcMPx antioxidant pathway and to determine the influence of the cellular context (physiological versus oxidative stress). Through co-precipitation coupled with a mass spectrometry approach, a variety of proteins were detected under physiological and oxidative stress conditions. Interestingly, functional category analysis of the proteins identified under physiological conditions showed that they were involved in the stress response, oxidoreduction, thiol transfer, and metabolic processes; this profile is distinct under oxidative stress conditions likely due to structural alterations. Our findings help to elucidate the reactions involving TcMPx and most importantly also reveal that this protein is present throughout the cell and that its interaction partners change following oxidative stress exposure. The involvement and significance of the proteins found to interact with TcMPx and other possible functions for this protein are discussed widening our knowledge regarding T. cruzi mitochondrial antioxidant defenses.


Subject(s)
Mitochondria/enzymology , Mitochondrial Proteins/metabolism , Peroxidases/metabolism , Protozoan Proteins/metabolism , Trypanosoma cruzi/metabolism , Carrier Proteins/genetics , Carrier Proteins/metabolism , Chromatography, Liquid , Electrophoresis, Gel, Two-Dimensional , Hydrogen Peroxide/pharmacology , Microscopy, Confocal , Mitochondrial Proteins/genetics , Oxidants/pharmacology , Peroxidases/genetics , Protein Binding/drug effects , Protein Interaction Maps , Proteome/genetics , Proteome/metabolism , Proteomics/methods , Protozoan Proteins/genetics , Tandem Mass Spectrometry , Trypanosoma cruzi/enzymology , Trypanosoma cruzi/genetics
3.
Toxicon ; 60(5): 773-81, 2012 Oct.
Article in English | MEDLINE | ID: mdl-22750534

ABSTRACT

A basic phospholipase A2 (LmrTX) isoform was isolated from Lachesis muta rhombeata snake venom and partially characterized. The venom was fractionated by molecular exclusion chromatography in ammonium bicarbonate buffer followed by reverse-phase HPLC on a C-5 Discovery® Bio Wide column. From liquid chromatography-electrospray ionization/mass spectrometry, the molecular mass of LmrTX was measured as 14.277.50 Da. The amino acid sequence showed a high degree of homology between PLA2 LmrTX from L. muta rhombeata and other PLA2 from snake venoms, like CB1 and CB2 from Crotalus durissus terrificus; LmTX-I and LmTX-II from Lachesis muta muta. LmrTX had PLA2 activity in the presence of a synthetic substrate and alkylation of histidine residues significantly inhibited (P < 0.05) the enzymatic activity of LmrTX and its anticoagulant and antithrombotic activity. In this study, we examined the ability of the LmrTX in altering thrombus formation in living mouse, using a photochemically induced arterial thrombosis model. The control animals that did not receive protein injection showed a normal occlusion time, which was around 57 ± 7.8 min. LmrTX, the PLA2 from L. muta rhombeata venom, caused a change in the occlusion time to 99 ± 10 min with doses of 7.5 µg/mice. Additionally, LmrTX showed the anticoagulant activity in vitro and ex vivo and prolonging the time aggregation in wash platelet induced by ADP and Thrombin.


Subject(s)
Crotalid Venoms/enzymology , Phospholipases A2/genetics , Phospholipases A2/metabolism , Thrombosis/chemically induced , Amino Acid Sequence , Animals , Base Sequence , Chromatography, Gel , Chromatography, High Pressure Liquid , Chromatography, Liquid , Mass Spectrometry , Mice , Molecular Sequence Data , Sequence Analysis, DNA , Species Specificity
4.
J Eur Acad Dermatol Venereol ; 17(1): 14-9, 2003 Jan.
Article in English | MEDLINE | ID: mdl-12602961

ABSTRACT

OBJECTIVES: The aim of this investigation is to compare the relative proportions of disaccharides of chondroitinase-digestible glycosaminoglycans (GAGs) among the different body sites in control human skin and in the skin lesions of patients with localized scleroderma. METHODS: The disaccharide relative proportions were determined using high-performance liquid chromatography (HPLC). RESULTS: DeltaDi-4S, the main disaccharide unit of dermatan sulphate (DS), was the major skin GAG disaccharide (approximately 70% of the total) in control skin among all different body sites studied here. In scleroderma there was an increase in the relative proportion of both deltaDi-HA, the main disaccharide unit of hyaluronic acid (HA), and deltaDi-diS(B) (alpha-deltaUA(2SO4)-1-->3-GalNAc(4SO4)), derived from DS, and a decrease in deltaDi-4S, as compared with the uninvolved skin or the site-matched control skin. CONCLUSION: DS is the major GAG species in normal skin from different body sites. In addition, our results suggest a decrease and also a structural change in DS and an increase in the proportion of HA in scleroderma skin.


Subject(s)
Glycosaminoglycans/metabolism , Scleroderma, Localized/metabolism , Skin/chemistry , Adult , Case-Control Studies , Chromatography, High Pressure Liquid , Dermatan Sulfate/analysis , Female , Humans , Hyaluronic Acid/analysis , Male
5.
Glycobiology ; 11(6): 433-40, 2001 Jun.
Article in English | MEDLINE | ID: mdl-11445548

ABSTRACT

Unfertilized eggs of the sea urchin Strongylocentrotus purpuratus are surrounded by a gelatinous layer rich in sulfated fucan. Shortly after fertilization this polysaccharide disappears, but 24 h later the embryos synthesize high amounts of dermatan sulfate concomitantly with the mesenchyme blastula-early gastrula stage when the larval gut is forming. This glycosaminoglycan has the same backbone structure [4-alpha-L-IdoA-1-->3-beta-D-GalNAc-1](n) as the mammalian counterpart but possesses a different sulfation pattern. It has a high content of 4-O- and 6-O-disulfated galactosamine units. In addition, chains of this dermatan sulfate are considerable longer than those of vertebrate tissues. Adult sea urchin tissues contain high concentrations of sulfated polysaccharides, but dermatan sulfate is restricted to the adult body wall where it accounts for approximately 20% of the total sulfated polysaccharides. In addition, sulfation at the 4-O-position decreases markedly in the dermatan sulfate from adult sea urchin when compared with the glycan from larvae. Overall, these results demonstrate the occurrence of dermatan sulfates with unique sulfation patterns in this marine invertebrate. The physiological implication of these oversulfated dermatan sulfates is unclear. One hypothesis is that interactions between components of the extracellular matrix in marine invertebrates occur at higher salt concentrations than in vertebrates and therefore require glycosaminoglycans with increased charge density.


Subject(s)
Acetylgalactosamine/analysis , Dermatan Sulfate/isolation & purification , Sea Urchins/embryology , Acetylgalactosamine/analogs & derivatives , Animals , Chromatography, High Pressure Liquid , Embryo, Nonmammalian , Nuclear Magnetic Resonance, Biomolecular , Tissue Distribution
6.
Braz J Med Biol Res ; 34(2): 251-8, 2001 Feb.
Article in English | MEDLINE | ID: mdl-11175502

ABSTRACT

Astroglial cells derived from lateral and medial midbrain sectors differ in their abilities to support neuritic growth of midbrain neurons in cocultures. These different properties of the two types of cells may be related to the composition of their extracellular matrix. We have studied the synthesis and secretion of sulfated glycosaminoglycans (GAGs) by the two cell types under control conditions and beta-D-xyloside-stimulated conditions, that stimulate the ability to synthesize and release GAGs. We have confirmed that both cell types synthesize and secrete heparan sulfate and chondroitin sulfate. Only slight differences were observed between the proportions of the two GAGs produced by the two types of cells after a 24-h labeling period. However, a marked difference was observed between the GAGs produced by the astroglial cells derived from lateral and medial midbrain sectors. The medial cells, which contain derivatives of the tectal and tegmental midline radial glia, synthesized and secreted approximately 2.3 times more chondroitin sulfate than lateral cells. The synthesis of heparan sulfate was only slightly modified by the addition of beta-D-xyloside. Overall, these results indicate that astroglial cells derived from the two midbrain sectors have marked differences in their capacity to synthesize chondroitin sulfate. Under in vivo conditions or a long period of in vitro culture, they may produce extracellular matrix at concentrations which may differentially affect neuritic growth.


Subject(s)
Astrocytes/metabolism , Glycosaminoglycans/biosynthesis , Mesencephalon/metabolism , Sulfates/metabolism , Animals , Cell Culture Techniques , Chondroitin Sulfates/biosynthesis , Chondroitin Sulfates/metabolism , Electrophoresis, Agar Gel , Glycosaminoglycans/metabolism , Heparitin Sulfate/biosynthesis , Heparitin Sulfate/metabolism , Mesencephalon/cytology , Mice
7.
Braz. j. med. biol. res ; 34(2): 251-258, Feb. 2001.
Article in English | LILACS | ID: lil-281604

ABSTRACT

Astroglial cells derived from lateral and medial midbrain sectors differ in their abilities to support neuritic growth of midbrain neurons in cocultures. These different properties of the two types of cells may be related to the composition of their extracellular matrix. We have studied the synthesis and secretion of sulfated glycosaminoglycans (GAGs) by the two cell types under control conditions and ß-D-xyloside-stimulated conditions, that stimulate the ability to synthesize and release GAGs. We have confirmed that both cell types synthesize and secrete heparan sulfate and chondroitin sulfate. Only slight differences were observed between the proportions of the two GAGs produced by the two types of cells after a 24-h labeling period. However, a marked difference was observed between the GAGs produced by the astroglial cells derived from lateral and medial midbrain sectors. The medial cells, which contain derivatives of the tectal and tegmental midline radial glia, synthesized and secreted ~2.3 times more chondroitin sulfate than lateral cells. The synthesis of heparan sulfate was only slightly modified by the addition of ß-D-xyloside. Overall, these results indicate that astroglial cells derived from the two midbrain sectors have marked differences in their capacity to synthesize chondroitin sulfate. Under in vivo conditions or a long period of in vitro culture, they may produce extracellular matrix at concentrations which may differentially affect neuritic growth


Subject(s)
Animals , Mice , Astrocytes/metabolism , Glycosaminoglycans/biosynthesis , Mesencephalon/cytology , Sulfates/metabolism , Sulfuric Acid Esters , Astrocytes/metabolism , Cell Culture Techniques , Chondroitin Sulfates/biosynthesis , Chondroitin Sulfates/metabolism , Electrophoresis, Agar Gel , Glycosaminoglycans/metabolism , Heparitin Sulfate/biosynthesis , Heparitin Sulfate/metabolism
8.
Insect Biochem Mol Biol ; 31(1): 31-40, 2001 Jan.
Article in English | MEDLINE | ID: mdl-11102832

ABSTRACT

We have characterized sulfated glycosaminoglycans from ovaries of the blood-sucking insect Rhodnius prolixus, and determined parameters of their synthesis and distribution within this organ by biochemical and histochemical procedures. The major sulfated glycosaminoglycan is heparan sulfate while chondroitin 4-sulfate is a minor component. These glycosaminoglycans are concentrated in the ovarian tissue and are not found inside the oocytes. Besides this, we detected the presence of a sulfated compound distinguished from sulfated glycosaminoglycans and possibly derived from sulfated proteins. Conversely to the compartmental location of sulfated glycosaminoglycans, the unidentified sulfated compound is located in the ovarian tissue as well as inside the oocytes. Based on these and other findings, the possible roles of ovarian sulfated glycosaminoglycans on the process of oogenesis in these insects are discussed.


Subject(s)
Glycosaminoglycans/metabolism , Rhodnius/metabolism , Animals , Chondroitin Sulfates/metabolism , Female , Heparitin Sulfate/metabolism , Isotope Labeling , Male , Ovary/metabolism , Staining and Labeling/methods , Sulfur Radioisotopes
9.
J Periodontal Res ; 35(3): 158-64, 2000 Jun.
Article in English | MEDLINE | ID: mdl-10929870

ABSTRACT

Glycosaminoglycans in normal and cyclosporin-induced gingival overgrowth were extracted by papain digestion and purified by Mono Q-FPLC chromatography. The purified glycosaminoglycans were analyzed by agarose gel electrophoresis and by the pattern of degradation products formed by chondroitin lyases on HPLC chromatography. Our results on the glycosaminoglycan composition showed presence of chondroitin 4- and 6-sulfate, dermatan sulfate, heparan sulfate and hyaluronic acid in both normal gingiva and cyclosporin-induced gingival overgrowth. The total and relative amounts of glycosaminoglycans were similar between normal and overgrown gingiva. This suggests that the glycosaminoglycan composition is not changed in cyclosporin-induced gingival overgrowth. Our present biochemical results conflict with histochemical and biosynthetic data previously reported by other groups. Those studies suggested that the affected tissues contained higher levels of glycosaminoglycans and that cyclosporin induced comparably high levels of these compounds in in vitro cultures of gingival fibroblasts. Therefore, these discrepant results suggest that a cyclosporin-induced increase on gingival glycosaminoglycans still remains an open question. The implications of these conflicting results are discussed.


Subject(s)
Cyclosporine/adverse effects , Gingival Overgrowth/chemically induced , Gingival Overgrowth/metabolism , Glycosaminoglycans/analysis , Immunosuppressive Agents/adverse effects , Adult , Chondroitin Sulfates/analysis , Chromatography, High Pressure Liquid , Dermatan Sulfate/analysis , Electrophoresis, Agar Gel , Extracellular Matrix Proteins/analysis , Heparitin Sulfate/analysis , Humans , Hyaluronic Acid/analysis , Middle Aged
10.
J Cell Physiol ; 185(1): 68-79, 2000 Oct.
Article in English | MEDLINE | ID: mdl-10942520

ABSTRACT

We determined the synthesis and secretion of glycosaminoglycans by three distinct preparations of mouse cultured thymic epithelial cells. These comprised primary cultures of thymic nurse cells (TNCs), which are normally located within the cortex of the thymic lobules, as well as two murine thymic epithelial cells, bearing a mixed, yet distinct, cortico-medullary phenotype. We first identified and measured the relative proportions of the various glycosaminoglycans in the three epithelial cells. Non-sulfated glycosaminoglycans are preponderantly secreted by the TNCs, while the sulfated glycans (particularly heparan sulfate) are relatively more abundant on the cell surface. The three types of epithelial cells differ markedly in their heparan sulfate composition, mainly due to different patterns of N- and O-sulfation. In addition, the cells differ in the synthesis and secretion of other glycosaminoglycans. Thus, TNCs secrete high amounts of dermatan sulfate + chondroitin sulfate to the culture medium. IT-76M1 cells secrete high proportions of heparan sulfate while 2BH4 cells show a more equilibrated proportion of dermatan sulfate/chondroitin sulfate and heparan sulfate. The three epithelial cells also differ in their capacity to produce hyaluronic acid and 2BH4 cells are distinguished by their high rate of synthesis of this glycosaminoglycan. In conclusion, our results show that distinct thymic epithelial cells can synthesize different types of glycosaminoglycans. Although it remains to be definitely determined whether these differences reflect the in vivo situation, our data provide new clues for further understanding of how glycosaminoglycan-mediated interactions behave in the thymus.


Subject(s)
Epithelial Cells/cytology , Epithelial Cells/physiology , Glycosaminoglycans/metabolism , Thymus Gland/cytology , Thymus Gland/physiology , Animals , Cell Differentiation/genetics , Genetic Variation , Mice , Mice, Inbred BALB C
11.
Biol Cell ; 92(6): 421-7, 2000 Sep.
Article in English | MEDLINE | ID: mdl-11132703

ABSTRACT

We determined the amounts of [35S]-glycosaminoglycans (GAGs) found on the intracellular, pericellular and extracellular compartments of primary cultures of astrocytes derived from newborn rat cortex and cerebellum in vitro. Our results show that the greatest portion of newly synthesized GAGs were found in different cellular compartments, depending on the source of the astrocytes. In the cells derived from the cerebellum, the proportion of [35S]-GAGs secreted to the culture medium preponderates over the amount found in the two other compartments, whereas cells derived from the cortex accumulated higher proportions of [35S]-GAGs in the intracellular compartment than in the two other compartments. Cortical and cerebellar glial cells synthesised and secreted heparan sulfate (HS) and chondroitin 4-sulfate (C-4S). HS was predominantly accumulated on the pericellular surface, while C-4S was mostly secreted to the culture medium. Beside the difference on the distribution of total [35S]-GAGs among the three cellular compartments, no difference was observed on the relative proportions of HS and C-4S within each compartment. By defining the source of GAGs, the present study may help to complement and extend information on biosynthesis of these compounds by mammalian glial cells.


Subject(s)
Astrocytes/metabolism , Glycosaminoglycans/biosynthesis , Glycosaminoglycans/metabolism , Animals , Autoradiography , Cell Compartmentation , Cell Culture Techniques , Cerebellar Cortex/cytology , Cerebellum/cytology , Chondroitin Sulfates/biosynthesis , Chondroitin Sulfates/metabolism , Heparitin Sulfate/biosynthesis , Heparitin Sulfate/metabolism , Neuroglia/metabolism , Rats , Sulfur Radioisotopes
12.
J Cell Physiol ; 178(1): 51-62, 1999 Jan.
Article in English | MEDLINE | ID: mdl-9886490

ABSTRACT

Epithelial cells are important components of the thymus microenvironment and are involved in thymocyte differentiation. The production and secretion of sulfated glycosaminoglycans by these cells grown in culture were investigated using labeling with radioactive 35S-Na2SO4 and 3H-glucosamine. The major glycosaminoglycans synthesized by these cells are heparan sulfate and hyaluronic acid. The structure of the heparan sulfate was investigated by the pattern of degradation products formed by deaminative cleavage with nitrous acid. The ratio 35S-sulfate/ H-glucosamine is high in the segments of the heparan sulfate released during the deaminative cleavage with nitrous acid but low in the resistant portion of the molecule. Thus, the heparan sulfate synthesized by the thymic epithelial cells contains a highly sulfated region. Digestion with heparitinase reveals that this highly sulfated region is a heparin-like segment of the molecule. The heparan sulfate is rapidly incorporated into the cell surface but its secretion to the extracellular medium requires a longer incubation period. Finally, heparin was used to mimic the possible effect of this heparan sulfate with a highly sulfated region, as ascertained by its ability to modulate thymocyte adhesion to thymic epithelial cells. Since heparin actually enhanced thymocyte adhesion, it is suggested that the heparan sulfate described herein, secreted by the thymic epithelium, may play a role upon intrathymic heterotypic cellular interactions.


Subject(s)
Epithelial Cells/metabolism , Heparitin Sulfate/biosynthesis , Heparitin Sulfate/metabolism , Sulfur/metabolism , Thymus Gland/cytology , Animals , Cell Fractionation , Cell Line , Chondroitin Sulfates/biosynthesis , Chondroitin Sulfates/metabolism , Clinical Enzyme Tests , Disaccharides/metabolism , Epithelial Cells/cytology , Female , Glycosaminoglycans/metabolism , Hyaluronic Acid/biosynthesis , Hyaluronic Acid/metabolism , Mice , Mice, Inbred BALB C , Oligosaccharides/metabolism , Polysaccharide-Lyases/pharmacology , Sulfur Radioisotopes , Time Factors , Tritium
13.
J Biol Chem ; 273(43): 27848-57, 1998 Oct 23.
Article in English | MEDLINE | ID: mdl-9774395

ABSTRACT

Dermatan sulfates with the same backbone structure [4-alpha-L-IdceA-1-->3-beta-D-GalNAc-1]n but with different patterns of sulfation substitutions have been isolated from the ascidian body. All the ascidian dermatan sulfates have a high content of 2-O-sulfated alpha-L-iduronic acid residues but differ in the pattern of sulfation of the N-acetyl-beta-D-galactosamine units. Styela plicata and Halocynthia pyriformis have 4-O-sulfated units, but in Ascidian nigra they are 6-O-sulfated. This collection of ascidian dermatan sulfates (together with native and oversulfated mammalian dermatan sulfate), where the extent and position of sulfate substitution have been fully characterized, were tested in anticoagulant assays. Dermatan sulfate from A. nigra has no discernible anticoagulant activity, which indicates that 4-O-sulfation of the N-acetyl-beta-D-galactosamine is essential for the anticoagulant activity of this glycosaminoglycan. In contrast dermatan sulfates from S. plicata and H. pyriformis are potent anticoagulants due to potentiation of thrombin inhibition by heparin cofactor II. These ascidian dermatan sulfates have approximately 10-fold and approximately 6-fold higher activity with heparin cofactor II than native and an oversulfated mammalian dermatan sulfate, respectively. They have no effect on thrombin or factor Xa inhibition by antithrombin. These naturally oversulfated ascidian dermatan sulfates are sulfated at selected sites required for interaction with heparin cofactor II and thus have specific and potent anticoagulant activity.


Subject(s)
Anticoagulants/isolation & purification , Dermatan Sulfate/isolation & purification , Sulfuric Acid Esters/isolation & purification , Urochordata/chemistry , Acetylgalactosamine/analogs & derivatives , Animals , Anions , Antithrombins/pharmacology , Disaccharides/analysis , Factor Xa/metabolism , Heparin Cofactor II , Iduronic Acid/analogs & derivatives , Nuclear Magnetic Resonance, Biomolecular , Partial Thromboplastin Time , Species Specificity , Thrombin/metabolism
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