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1.
PLoS Comput Biol ; 15(2): e1006733, 2019 02.
Article in English | MEDLINE | ID: mdl-30818329

ABSTRACT

Metabolic reprogramming is a general feature of cancer cells. Regrettably, the comprehensive quantification of metabolites in biological specimens does not promptly translate into knowledge on the utilization of metabolic pathways. By estimating fluxes across metabolic pathways, computational models hold the promise to bridge this gap between data and biological functionality. These models currently portray the average behavior of cell populations however, masking the inherent heterogeneity that is part and parcel of tumorigenesis as much as drug resistance. To remove this limitation, we propose single-cell Flux Balance Analysis (scFBA) as a computational framework to translate single-cell transcriptomes into single-cell fluxomes. We show that the integration of single-cell RNA-seq profiles of cells derived from lung adenocarcinoma and breast cancer patients into a multi-scale stoichiometric model of a cancer cell population: significantly 1) reduces the space of feasible single-cell fluxomes; 2) allows to identify clusters of cells with different growth rates within the population; 3) points out the possible metabolic interactions among cells via exchange of metabolites. The scFBA suite of MATLAB functions is available at https://github.com/BIMIB-DISCo/scFBA, as well as the case study datasets.


Subject(s)
Computational Biology/methods , Sequence Analysis, RNA/methods , Single-Cell Analysis/methods , Adenocarcinoma of Lung/genetics , Algorithms , Breast Neoplasms/genetics , Computer Simulation , Female , Gene Expression Profiling/methods , Genetics, Population/methods , Humans , Male , Metabolic Networks and Pathways , Neoplasms/genetics , Neoplasms/metabolism , RNA/genetics , Software , Transcriptome/genetics
2.
PLoS Comput Biol ; 13(9): e1005758, 2017 Sep.
Article in English | MEDLINE | ID: mdl-28957320

ABSTRACT

Cancer cells share several metabolic traits, including aerobic production of lactate from glucose (Warburg effect), extensive glutamine utilization and impaired mitochondrial electron flow. It is still unclear how these metabolic rearrangements, which may involve different molecular events in different cells, contribute to a selective advantage for cancer cell proliferation. To ascertain which metabolic pathways are used to convert glucose and glutamine to balanced energy and biomass production, we performed systematic constraint-based simulations of a model of human central metabolism. Sampling of the feasible flux space allowed us to obtain a large number of randomly mutated cells simulated at different glutamine and glucose uptake rates. We observed that, in the limited subset of proliferating cells, most displayed fermentation of glucose to lactate in the presence of oxygen. At high utilization rates of glutamine, oxidative utilization of glucose was decreased, while the production of lactate from glutamine was enhanced. This emergent phenotype was observed only when the available carbon exceeded the amount that could be fully oxidized by the available oxygen. Under the latter conditions, standard Flux Balance Analysis indicated that: this metabolic pattern is optimal to maximize biomass and ATP production; it requires the activity of a branched TCA cycle, in which glutamine-dependent reductive carboxylation cooperates to the production of lipids and proteins; it is sustained by a variety of redox-controlled metabolic reactions. In a K-ras transformed cell line we experimentally assessed glutamine-induced metabolic changes. We validated computational results through an extension of Flux Balance Analysis that allows prediction of metabolite variations. Taken together these findings offer new understanding of the logic of the metabolic reprogramming that underlies cancer cell growth.


Subject(s)
Cell Proliferation , Glucose/metabolism , Glutamine/metabolism , Lactic Acid/biosynthesis , Metabolic Networks and Pathways , Models, Biological , Neoplasms/metabolism , Animals , Computer Simulation , Humans , Metabolic Flux Analysis , Neoplasms/pathology
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