[Floating thrombus of the internal carotid artery as a rare complication in ovarian hyperstimulation syndrome after in vitro fertilization/embryo transfer]. / Flottierender Thrombus der Arteria carotis interna als seltene Komplikation bei einem ovariellen Hyperstimulationssyndrom nach In-vitro-Fertilisation/Embryotransfer.

We report the successful thrombectomy of the internal carotid artery. A 33-year-old woman suffered from ovarian hyperstimulation syndrome after treatment with FSH/HCG for in-vitro fertilization. She developed a transient left hemiparesis. This was caused by a floating thrombus located in the internal carotid artery. Open surgical treatment with thrombectomy prevents recurrent neurological symptoms.

In vitro sensitivity testing of cells of histologically typified ovarian carcinomas and carcinoma of corpus with 3H-cyclophosphamide-metabolites in the serum of man after massive dose therapy.

Cells of a carcinoma of the uterine corpus and of five ovarian carcinomas were cultured in vitro with monolayer and sandwich methods, respectively. After 24-hours-incubation with serum samples of patients treated with a massive dose of 3H-cyclophosphamide (60 mg/kg body weight i.v.)-the samples were taken at different times after application-these cultures with a chromatographically defined content of free metabolites were interpreted, guided by morphological criteria. Incubation of the cultures with serum samples taken 5 hours after application of the massive dose turned out to be a practical system for detection, before the start of therapy, of the sensitivity of malignant tumors to cyclophosphamide. Statements about the proliferation kinetics of the malignant cells, about the active ultimate form of the antitumor agent and its way of action, which are not guaranteed experimentally, are largely eliminated by this method. The justification for using 4-hydroxy- and 4-hydroperoxy-cyclophosphamide to test the sensitivity of malignant cells in vitro to cyclophosphamide was critically examined.

[Tests of the sensitivity of histologically characterized carcinomas of the ovary or corpus to 4-hydroperoxicyclophosphamide, amethopterine, mitopodozide and VM-26 sandoz, using the technique of primary culture (author's transl)]. / Sensibilitätstestung histologisch charakterisierter Ovarial- und Korpuskarzinome gegen 4-Hydroperoxicyclophosphamid, Amethopterin, Mitopodozid und 4-O-Dimethyl-1-O-(4,6-O-2-thenyliden-ss-D-glucopyranosyl)epipodophyllotoxin mit der Primärkulturtechnik.

Cells from three carcinomas of the uterine corpus and from 14 ovarian carcinomas have been cultured in vitro by the monlayer and sandwich methods. They were evaluated on the basis of morphological criteria after 24 hours of incubation with 4-hydroperoxicyclophosphamide, amethopterine, mitopodozide and VM-26 Sandoz, using two dose ranges in each case. The index of 3H-thymidine was determined by means of two parallel experiments. Later results concerning pharmacokinetics of the cyclophosphamide have lead us to apply the technique of primary culture. Owing to the long duration of the incubation period, the 4-hydroperoxicyclophosphamide with its secondary and degradation products can influence the greatest possible numbers of cells through several phases of the cell cycle, thus obtaining an approach to in-vivo kinetics. A concentration of substrate per unit of volume, higher than the actually in vivo expected, is to compensate the short half-life of 4-hydroperoxicyclophosphamide as well as the lack of the supply-function of current dynamics. 4-hydroperoxicyclophosphamide was the cytostatic agent with the largest range of effectiveness, followed by amethopterine, while the mitotic poisons mitopodozide and VM-26 Sandoz were less effective. The histology and degree of differentiating of the tumors as well as the proliferation rate are not correlated in a distinct manner with sensitivity to cytostatics. Pretreatment with cytostatics of with irradiation revealed no certain influence upon subsequent sensitivity to cytostatics.

[Isoelectric focusing of an alkylating 3H-cyclophosphamide derivative from human bladder urine]. / Untersuchungen über einen alkylierenden 3H-Cyclophosphamidabkömmling des menschlichen Blasenurins unter Anwendung der isoelektrischen Fokussierung

1) A stable polar 3H-cyclophosphamide metabolite capable of alkylating 4-(p-nitrobenzyl) pyridine and relatively high concentrated in human bladder urine was isolated. The compound has the pI value 9.06. It is a phosphoramide mustard conjugate. 2) Determination of the isoelectric point was accomplished by means of a new thin-layer isoelectric focusing technique applicable for characterizing loaded parts of low molecular weight substance mixtures.