Degradation of a mixture of 13 polycyclic aromatic hydrocarbons by commercial effective microorganisms.

The study focused on the contribution of effective microorganisms (EM) and their consortia, used in commercial biological preparations and formulations for soil revitalization, to the degradation of a mixture of 13 polycyclic aromatic hydrocarbons (PAHs) commonly found in the soil environment. PAHs, diverse forms of which are present in the environment, never occur individually but always as a part of a chemical mixture. Therefore, the research presented in this article, focusing on the EM impact on the mixture of PAHs, reflects the conditions most similar to natural ones. On Day 35 of the experiment, PAH levels decreased by 75.5-95.5%. The highest PAHs degradation efficiency was achieved for fluorene, with a preparation containing eight bacteria strains from the Bacillus genus: B. coagulans, B. amyloliquefaciens, B. laterosporus, B. licheniformis, B. mucilaginosus, B. megaterium, B. polymyxa, and B. pumilus. All tested preparations containing bacterial consortia and a preparation with the yeast S. cerevisiae intensified the PAHs degradation more effectively than formulations including only the yeast Yarrowia lipolytica or a mixture of Debaryomyces hansenii and Bacillus. The designed and proposed research will contribute to the development of biotechnological methods - bioremediation by microorganisms that are safe for the human and environment health.

Effect of Three Commercial Formulations Containing Effective Microorganisms (EM) on Diflufenican and Flurochloridone Degradation in Soil.

The aim of this study was to determine the influence of effective microorganisms (EM) present in biological formulations improving soil quality on degradation of two herbicides, diflufenican and flurochloridone. Three commercially available formulations containing EM were used: a formulation containing Bifidobacterium, Lactobacillus, Lactococcus, Streptococcus, Bacillus, and Rhodopseudomonas bacteria and the yeast Saccharomyces cerevisiae; a formulation containing Streptomyces, Pseudomonas, Bacillus, Rhodococcus, Cellulomonas, Arthrobacter, Paenibacillusa, and Pseudonocardia bacteria; and a formulation containing eight strains of Bacillus bacteria, B. megaterium, B. amyloliquefaciens, B. pumilus, B. licheniformis, B. coagulans, B. laterosporus, B. mucilaginosus, and B. polymyxa. It was demonstrated that those formulations influenced degradation of herbicides. All studied formulations containing EM reduced the diflufenican degradation level, from 35.5% to 38%, due to an increased acidity of the soil environment and increased durability of that substance at lower pH levels. In the case of flurochloridone, all studied EM formulations increased degradation of that active substance by 19.3% to 31.2% at the most. For control samples, equations describing kinetics of diflufenican and flurochloridone elimination were plotted, and a time of the half-life of these substances in laboratory conditions was calculated, amounting to 25.7 for diflufenican and 22.4 for flurochloridone.