ABSTRACT
Two identical isolates were recovered in pure culture from the blood and urine of a patient suffering from severe septicaemia associated with obstructive pyelonephritis secondary to lithotripsy. Preliminary phenotypic and genotypic characterizations based on serological, biochemical and sequence analyses following PCR amplification of selected gene regions indicate that this organism represents a potential new Francisella genomic species.
Subject(s)
Bacteremia/microbiology , Blood/microbiology , Francisella/genetics , Francisella/isolation & purification , Gram-Negative Bacterial Infections/microbiology , Urine/microbiology , Adult , Bacterial Typing Techniques , DNA, Bacterial/analysis , DNA, Bacterial/genetics , Electrophoresis, Polyacrylamide Gel , Francisella/classification , Genes, rRNA , Humans , Interspersed Repetitive Sequences/genetics , Lithotripsy/adverse effects , Male , Molecular Sequence Data , Phylogeny , Polymerase Chain Reaction , Polymorphism, Single Nucleotide , Pyelonephritis/etiology , RNA, Ribosomal, 16S/genetics , Sequence AlignmentSubject(s)
Selective Serotonin Reuptake Inhibitors/adverse effects , Serotonin Syndrome/diagnosis , Sertraline/adverse effects , Spinal Cord Compression/diagnosis , Aged , Aged, 80 and over , Depression/drug therapy , Diagnosis, Differential , Humans , Male , Prostatic Neoplasms/psychology , Serotonin Syndrome/chemically induced , Spinal Neoplasms/secondaryABSTRACT
We describe an immunocompetent adolescent who presented with exceptionally severe Bordetella holmesii infection, including previously undescribed manifestations. Sequelae included a severe restrictive lung defect due to pulmonary fibrosis.
Subject(s)
Bordetella Infections/diagnosis , Bordetella/isolation & purification , Genes, rRNA , RNA, Ribosomal, 16S/genetics , Sequence Analysis, DNA , Adolescent , Bordetella/classification , Bordetella/genetics , Bordetella Infections/microbiology , Female , Genes, Bacterial , Humans , Immunocompetence , Molecular Sequence DataABSTRACT
In Escherichia coli K-12, the shiA gene is involved in the uptake of shikimate. This gene has been cloned and its nucleotide sequence determined. The gene is predicted to encode a protein of 438 amino acids and lies adjacent to the amn gene. The hydropathy profile and the amino acid sequence indicate that the ShiA protein is a polytopic membrane protein that shows a homology with members of the major facilitator superfamily of transport proteins. Recombining an inactive form of the cloned gene into the chromosome creates mutants unable to transport shikimate. Introducing a wild-type gene on a multicopy plasmid into a shiA mutant restores the ability to transport shikimate. When this multicopy shiA plasmid is introduced into an aroE strain, this strain is now able to grow with shikimate as the aromatic supplement, consistent with the notion that dehydroshikimate (DHS) accumulated in an aroE strain prevents uptake of shikimate by competition. Expression of the shiA gene does not appear to be regulated by the TyrR protein, a repressor/activator that controls the expression of other genes involved with the biosynthesis or transport of the aromatic amino acids.
Subject(s)
Bacterial Proteins/biosynthesis , Carrier Proteins/biosynthesis , Escherichia coli Proteins , Escherichia coli/genetics , Escherichia coli/metabolism , Membrane Transport Proteins , Amino Acid Sequence , Bacterial Proteins/chemistry , Bacterial Proteins/genetics , Carrier Proteins/chemistry , Carrier Proteins/genetics , Chromosome Mapping , Cloning, Molecular , Genes, Bacterial , Models, Molecular , Molecular Sequence Data , Open Reading Frames , Protein Structure, Secondary , Sequence Alignment , Sequence Homology, Amino Acid , Shikimic Acid/metabolismABSTRACT
In the course of sequencing the aroK gene, a number of errors were found in the published sequence. The corrected sequence alters the length of the aroK coding region such that the AroK and AroL proteins are now of comparable length and the homology between them extends the entire length of the two enzymes.
Subject(s)
Escherichia coli/genetics , Isoenzymes/genetics , Phosphotransferases (Alcohol Group Acceptor)/genetics , Amino Acid Sequence , Base Sequence , Cloning, Molecular , Escherichia coli/enzymology , Molecular Sequence Data , Mutagenesis , Protein Biosynthesis , Reading Frames/genetics , Recombinant Fusion Proteins/biosynthesis , Sequence Analysis, DNA , Sequence Homology, Amino Acid , Shikimic Acid/metabolismABSTRACT
pheV, one of the genes that code for tRNA(Phe), was deleted from the chromosome of a strain of Escherichia coli K-12. As a consequence of this mutation, expression of pheA, the gene for chorismate mutase P-prephenate dehydratase, the first enzyme in the terminal pathway of phenylalanine biosynthesis, was derepressed. Similar derepression of pheA has been reported in pheR mutants of E. coli K-12 (J. Gowrishankar and J. Pittard, J. Bacteriol. 150:1130-1137, 1982). Attempts to introduce a pheR mutation into the delta pheV strain failed under circumstances suggesting that this combination of mutations is lethal. Southern blot analysis of pheV+ and delta pheV strains indicated that there are only two tRNA(Phe) genes in E. coli. It is recommended that the names pheU and pheV be retained for these genes.
Subject(s)
Escherichia coli/genetics , Genes, Bacterial , Multigene Family , RNA, Transfer, Phe/genetics , Blotting, Southern , Chromosome Deletion , Chromosomes, Bacterial , DNA, Bacterial/genetics , DNA, Bacterial/isolation & purification , Genotype , Nucleic Acid Hybridization , Restriction MappingABSTRACT
Two cases of squamous cell carcinoma complicating longstanding acne conglobata in a father and daughter are described. The outcome was fatal in both cases.
Subject(s)
Acne Vulgaris/genetics , Carcinoma, Squamous Cell/genetics , Skin Neoplasms/genetics , Acne Vulgaris/complications , Acne Vulgaris/pathology , Carcinoma, Squamous Cell/complications , Carcinoma, Squamous Cell/pathology , Female , Humans , Middle Aged , Skin Neoplasms/complications , Skin Neoplasms/pathologyABSTRACT
A mutant strain of Escherichia coli K-12 that is defective in both the tyrosine-specific and phenylalanine-specific transport systems was isolated. The defects in these systems were shown to be due to mutations in two distinct loci, tyrP and pheP, respectively.
Subject(s)
Escherichia coli/metabolism , Genes , Phenylalanine/metabolism , Tyrosine/metabolism , Biological Transport, Active , Chromosome Mapping , Chromosomes, Bacterial , Escherichia coli/genetics , MutationABSTRACT
The regulation of the aromatic amino acid transport systems was investigated. The common (general) aromatic transport system and the tyrosine-specific transport system were found to be subject to repression control, thus confirming earlier reports. In addition, tryosine- and tryptophan-specific transport were found to be enhanced by growth of cells with phenylalanine. The repression and enhancement of the transport systems was abolished in a strain carrying an amber mutation in the regulator gene tyrR. This indicates that the tyrR gene product, which was previously shown to be involved in regulation of aromatic biosynthetic enzymes, is also involved in the regulation of the aromatic amino acid transport systems.
