Your browser doesn't support javascript.
loading
Show: 20 | 50 | 100
Results 1 - 20 de 31
Filter
Add more filters










Publication year range
2.
Mol Hum Reprod ; 12(12): 737-48, 2006 Dec.
Article in English | MEDLINE | ID: mdl-17020905

ABSTRACT

Adenomyosis is a common gynaecological disorder characterized by the abnormal growth of endometrium into the myometrium and myometrial hypertrophy/hyperplasia. Uterine fibroids are benign neoplasms of the myometrium, and they represent a diagnostic pitfall for adenomyosis. In this study, we have used the genome-wide Affymetrix U133 Plus 2.0 microarray platform to compare the gene expression patterns of adenomyosis, uterine fibroids, normal endometrium and myometrium. Unsupervised principal component analysis (PCA) revealed that these four tissue types could be segregated from one another solely based on their gene expression profiles. Analysis of variance (ANOVA), followed by Tukey means separation test, significance analysis of microarrays (SAM) and 2-fold change threshold, identified 7415 probe sets as differentially expressed among the four groups of samples. Supervised cluster analysis based on these probe sets clustered adenomyosis most closely with endometrium and uterine fibroids with myometrium, consistent with the anatomic origin of these two diseases. The Tukey means separation post hoc testing found 2073 probe sets altered between adenomyosis and normal endometrium or myometrium, and 2327 probe sets altered in expression when comparing uterine fibroids with myometrium. Using Ingenuity Pathways Analysis (IPA), we found 9 highly significant functional networks in adenomyosis and 10 in uterine fibroids. Notably, the top network in both cases was associated with functions implicated in cancer and cell death. Finally, we compared the gene expression profiles of adenomyosis and uterine fibroids and identified 471 differentially expressed probe sets that may represent potential biomarkers for the differential diagnosis of these diseases.


Subject(s)
Endometriosis/genetics , Gene Expression Profiling , Analysis of Variance , Biomarkers , Biomarkers, Tumor , Diagnosis, Differential , Endometriosis/diagnosis , Endometriosis/metabolism , Endometriosis/pathology , Endometrium/metabolism , Female , Humans , Leiomyoma/diagnosis , Leiomyoma/genetics , Leiomyoma/metabolism , Myometrium/metabolism , Neoplasm Proteins/biosynthesis , Neoplasm Proteins/genetics , Oligonucleotide Array Sequence Analysis , Principal Component Analysis , Uterine Neoplasms/diagnosis , Uterine Neoplasms/genetics , Uterine Neoplasms/metabolism
3.
Vet Rec ; 149(5): 156-7, 2001 Aug 04.
Article in English | MEDLINE | ID: mdl-11517987
4.
Cancer Genet Cytogenet ; 97(1): 25-31, 1997 Aug.
Article in English | MEDLINE | ID: mdl-9242214

ABSTRACT

The current World Health Organization (WHO) classification groups together both infratentorial neoplasms (medulloblastomas) and their supratentorial counterparts as primitive neuroectodermal tumors (PNETs), implying a common origin. Previous analyses of medulloblastoma have shown loss of chromosome arm 17p as the most frequent genetic abnormality: the molecular genetic constitution of supratentorial PNETS has not been systematically studied. We therefore examined 8 hemispheric PNETs and 35 medulloblastomas with 17p restriction fragment length polymorphism (RFLP) and microsatellite markers. We also examined the TP53 tumor suppressor gene by a combined polymerase chain reaction-denaturing gradient gel (PCR-DGGE) technique. Our results showed that all of the 17p markers tested were preserved in all of the supratentorial PNET specimens. In contrast, loss of distal chromosome arm 17p was detected in 37% of the medulloblastomas. Analysis of the TP53 gene showed 2 mutations in the medulloblastomas and no mutations in the supratentorial tumors. These results show that the most common molecular genetic abnormality in infratentorial PNETS is absent in their supratentorial counterparts and suggests that alternative pathways and genetic events may be involved in their etiology.


Subject(s)
Brain Neoplasms/genetics , Cerebellar Neoplasms/genetics , Chromosome Deletion , Chromosomes, Human, Pair 17/genetics , Genetic Heterogeneity , Medulloblastoma/genetics , Adolescent , Child , Child, Preschool , Female , Genes, p53 , Humans , Infant , Infant, Newborn , Male , Microsatellite Repeats , Mutation , Polymerase Chain Reaction , Polymorphism, Restriction Fragment Length
5.
Genes Chromosomes Cancer ; 17(1): 37-44, 1996 Sep.
Article in English | MEDLINE | ID: mdl-8889505

ABSTRACT

Ependymomas are glial tumors of the brain and spinal cord occurring both sporadically and in a familial syndrome, neurofibromatosis type 2 (NF2). Previous analyses performed on specimens obtained predominantly from adult patients have shown loss of DNA sequences from chromosome arm 22q, which is the location of the NF2 gene. Previously, we documented the consistent loss of chromosome arm 17p DNA in medulloblastoma and astrocytoma, which are the most common brain tumors in children. Although mutation of the TP53 gene located on 17p is the most frequent genetic mutation in all adult tumor types, such mutations are rare in most childhood brain tumors investigated to date. We studied a series of pediatric ependymoma specimens (16 intracranial and 2 spinal) for loss of 17p and 22q DNA sequences and for mutation of the TP53 and NF2 genes. None of the children had the clinical stigmata of NF2. We detected loss of 17p DNA sequences in 9 of the 18 specimens (50%); in 7 of 9 of these specimens (78%), the 144-D6 marker was deleted. In contrast, only 2 of these same 18 specimens (11%) showed loss of 22q DNA. One TP53 gene mutation was detected in a child from a cancer kindred. No mutations were detected in the NF2 gene. Our results suggest that loss of chromosome arm 17p DNA sequences is common in sporadic pediatric ependymomas and that, in contrast to ependymomas in adults, deletion of chromosome arm 22q sequences is rare. Furthermore, TP53 and NF2 gene mutations do not play an important role in the etiology of sporadic pediatric ependymomas.


Subject(s)
Brain Neoplasms/genetics , Chromosome Deletion , Chromosomes, Human, Pair 17 , Chromosomes, Human, Pair 22 , Ependymoma/genetics , Spinal Cord Neoplasms/genetics , Adolescent , Blotting, Southern , Child , Child, Preschool , Female , Genes, Neurofibromatosis 2/genetics , Genes, p53/genetics , Humans , Infant , Male , Microsatellite Repeats , Mutation , Polymorphism, Restriction Fragment Length , Sequence Analysis, DNA
6.
Poult Sci ; 73(6): 836-42, 1994 Jun.
Article in English | MEDLINE | ID: mdl-8072926

ABSTRACT

Six B complex recombinants, BR1 (F24-G23), BR2 (F2-G23), BR3 (F2-G23), BR4 (F2-G23), BR5 (F21-G19), and BR6 (F21-G23), from the fourth backcross generation to highly inbred line UCD 003 (B17B17) were studied for their response to Rous sarcomas. Eight hatches were produced from heterozygous (BRnB17) parents. Chicks were wingweb inoculated with 50 pock-forming units of Rous sarcoma virus (RSV) at 6 wk of age. A tumor profile index (TPI), based on degree of tumor regression, was evaluated by analysis of variance. BR2, BR3, and BR4 are serologically similar F2-G23 recombinants. Haplotype B2, the origin of BF2, is a known tumor regressor, yet BR2BR2 chickens had a significantly lower TPI than BR3BR3 and BR4BR4 chickens. The TPI of BR2BR2 (F2-G23) chickens was also significantly lower than the TPI of chickens homozygous for BR1 (F24-G23) and BR5 (F21-G19). The BR6BR6 (F21-G23) chickens had significantly lower TPI than all homozygotes except BR2BR2 (F2-G23). Among heterozygous genotypes, BR2B17, BR5B17, and BR6B17 differed significantly from BR1B17, BR3B17, and BR4B17. These results suggest that serologically similar recombinants that contain (F2-G23) possess different genes affecting tumor regression. In addition, degrees of tumor regression in BR5 (F21-G19) and BR6 (F21-G23), both of which contain BF21, may be due to genetic differences within the B-F/B-L or B-G regions.


Subject(s)
Chickens/immunology , Major Histocompatibility Complex/genetics , Neoplasm Regression, Spontaneous/immunology , Sarcoma, Avian/immunology , Animals , Haplotypes/genetics , Haplotypes/immunology , Neoplasm Regression, Spontaneous/genetics , Sarcoma, Avian/genetics , Sarcoma, Avian/pathology
7.
Biochem Pharmacol ; 43(11): 2359-68, 1992 Jun 09.
Article in English | MEDLINE | ID: mdl-1610401

ABSTRACT

Camphor, alpha-pinene (the major component of turpentine), and thujone (a constituent in the liqueur called absinthe) produced an increase in porphyrin production in primary cultures of chick embryo liver cells. In the presence of desferrioxamine (an iron chelator which inhibits heme synthesis and thereby mimics the effect of the block associated with acute porphyria), the terpenes enhanced porphyrin accumulation 5- to 20-fold. They also induced synthesis of the rate-controlling enzyme for the pathway, 5-aminolevulinic acid synthase, which was monitored both spectrophotometrically and immunochemically. These effects are shared by well-known porphyrogenic chemicals such as phenobarbital and glutethimide. Camphor and glutethimide alone led to the accumulation of mostly uro- and heptacarboxylporphyrins, whereas alpha-pinene and thujone resulted in lesser accumulations of porphyrins which were predominantly copro- and protoporphyrins. In the presence of desferrioxamine, plus any of the three terpenes, the major product that accumulated was protoporphyrin. The present results indicate that the terpenes tested are porphyrogenic and hazardous to patients with underlying defects in hepatic heme synthesis. There are also implications for the illness of Vincent van Gogh and the once popular, but now banned liqueur, called absinthe.


Subject(s)
Camphor/toxicity , Cytochrome P-450 Enzyme System , Famous Persons , Liver/drug effects , Monoterpenes , Terpenes/toxicity , 5-Aminolevulinate Synthetase/biosynthesis , Alcoholic Beverages/history , Alcoholic Beverages/toxicity , Animals , Bicyclic Monoterpenes , Cells, Cultured/drug effects , Chick Embryo , Deferoxamine/pharmacology , Dose-Response Relationship, Drug , Enzyme Induction/drug effects , Heme Oxygenase (Decyclizing)/biosynthesis , History, 19th Century , Liver/embryology , Liver/metabolism , Netherlands , Oxidoreductases, N-Demethylating/biosynthesis , Paintings/history , Porphyrias/chemically induced , Porphyrias/history , Porphyrins/metabolism , Terpenes/history
13.
16.
Article in English | MEDLINE | ID: mdl-2867853

ABSTRACT

The firing rates of single neurons were recorded in the septal and preoptic areas of unanesthetized rabbits during brief periods of ambient heating. The temporal response for neurons responsive to ambient temperature were calculated as the interval of time between the onset of heating and the point at which the unit's activity reached 63% (i.e. 1-1/e) of its total change in activity. Thirty-one neurons were isolated in 5 rabbits. Fourteen neurons were facilitated, 10 were inhibited and 7 were unaffected by heating. Temporal responses ranged from less than 5 to 122 s. We observed a bimodal relationship in the number of neuronal responses to ambient temperature vs temporal response: a sharp 0- to less than 10-s peak comprising 22% of all responses and a relatively broad peak with a mode of 60 to less than 70 s. These data resemble the temporal response patterns of thermally excitable neurons throughout the central nervous system.


Subject(s)
Body Temperature Regulation , Preoptic Area/physiology , Septum Pellucidum/physiology , Animals , Brain Mapping , Hot Temperature , Male , Rabbits , Reaction Time/physiology
17.
J Urol ; 132(3): 437-8, 1984 Sep.
Article in English | MEDLINE | ID: mdl-6471172

ABSTRACT

More than 200 patients have undergone percutaneous stone extraction at our institution. With the percutaneous nephrostomy tract as the conduit to the urinary tract we have removed 95 per cent of the pelviocaliceal stones and 80 per cent of the ureteral stones with grasping forceps, baskets, ultrasonic lithotripsy or a combination of these procedures. Complications were few and patients returned to work sooner than after an open operation.


Subject(s)
Kidney Pelvis/surgery , Urinary Calculi/surgery , Endoscopy , Female , Humans , Kidney Calculi/surgery , Male , Methods , Suction , Ultrasonic Therapy , Ureter , Ureteral Calculi/surgery , Urinary Catheterization
19.
South Med J ; 75(1): 9-13, 1982 Jan.
Article in English | MEDLINE | ID: mdl-7054889

ABSTRACT

Between 1944 and 1969, 192 women with carcinoma of the breast were treated at The University of Texas System Cancer Center M. D. Anderson Hospital and Tumor Institute by standard radical mastectomy for small outer quadrant lesions and pathologically negative axillary lymph nodes. Twenty percent of these patients with "localized disease" eventually had disseminated breast cancer. Various patient, hormonal, genetic, and pathologic factors were found to have little influence on the prognosis. Patterns of metastatic disease revealed that these patients did not have a unique form of breast cancer, but apparently an earlier stage in the spectrum of advancing disease. The results of this study appear to represent the best that can be obtained with regional therapy for carcinoma of the breast and emphasize the need for reliable adjuvant therapy.


Subject(s)
Breast Neoplasms/surgery , Carcinoma/surgery , Mastectomy , Adult , Aged , Breast Neoplasms/diagnosis , Carcinoma/diagnosis , Female , Humans , Middle Aged , Neoplasm Metastasis , Prognosis , Retrospective Studies , Time Factors
20.
Am J Clin Nutr ; 34(2): 199-203, 1981 Feb.
Article in English | MEDLINE | ID: mdl-7211724

ABSTRACT

In order to develop a reliable system for measuring daily cholesterol intake in individual outpatients, studies were undertaken to establish the shortest time period (in days) for which it is necessary to obtain daily food intake records. Three volunteers were trained in dietary record-keeping and portion-size assessment, and instructed to self-select a low-cholesterol diet for 20 days. During the study period they maintained daily dietary records and collected dummy diets. Comparisons of cholesterol intake calculated from the dietary records (mean 144 mg/day, SD +/- 13, n = 60) to the values from chemical analysis (118 +/- 28 mg/day) demonstrated that the calculated values were higher (mean 19%). More importantly, it was found that a minimum of 9 days' records of dummy diet analyses were required in order to reach an estimate of daily cholesterol intake that varied by less than 10% from the mean of the 20-days' values. In 100 outpatients trained to adhere to a moderately low-cholesterol intake and who maintained sequential dietary records for 9 days, it was found that the mean daily intake was 251 mg/day but that individual patients exhibited substantial daily variations in cholesterol intake (average coefficient of variation = 54%, range = 8.5 to 121.2%). These results demonstrate that, under conditions of training in dietary record-keeping and portion-size assessment, adherence to a low-cholesterol diet, and with collection of at least 9 days of dietary records, a reliable quantitative estimate of daily dietary cholesterol intake can be obtained in free-living outpatient populations.


Subject(s)
Cholesterol, Dietary/administration & dosage , Diet Surveys , Hyperlipidemias/metabolism , Nutrition Surveys , Ambulatory Care/methods , Cholesterol, Dietary/metabolism , Diet , Female , Food Analysis , Humans , Male
SELECTION OF CITATIONS
SEARCH DETAIL
...