From Classroom to Capitol: Building Advocacy Capacity Through State-Level Advocacy Experiences.

This commentary provides insight from Community Health Education and Master of Public Health students on the benefits of participating in a state-level Advocacy Experience and provides a theoretical framework for increased advocacy intention among students as a result of participating in a state-level Advocacy Experience. Providing students the opportunity to translate what they learn about advocacy in the classroom into advocacy in action with policy makers is vital to the career development of our future health education professionals and is key to increasing advocacy capacity within our profession. This article builds on previous work from emerging public health professionals highlighting the role of policy advocacy in professional development and provides additional perspectives from the next generation of health education specialists.

Historical Patterns and Drivers of Spatial Changes in Recreational Fishing Activity in Puget Sound, Washington.

Small-scale fisheries are the primary users of many coastal fish stocks; yet, spatial and temporal patterns of recreational and subsistence fishing in coastal marine ecosystems are poorly documented. Knowledge about the spatial distribution of fishing activities can inform place-based management that balances species conservation with opportunities for recreation and subsistence. We used a participatory mapping approach to document changes in spatial fishing patterns of 80 boat-based recreational anglers from 1950 to 2010 in Puget Sound, Washington, USA. Hand-drawn fishing areas for salmon, rockfishes, flatfishes, and crabs were digitized and analyzed in a Geographic Information System. We found that recreational fishing has spanned the majority of Puget Sound since the 1950s, with the heaviest use limited to small areas of central and northern Puget Sound. People are still fishing in the same places they were decades ago, with relatively little change in specific locations despite widespread declines in salmon and bottomfish populations during the second half of the 20th century. While the location of core fishing areas remained consistent, the size of those areas and intensity of use changed over time. The size of fishing areas increased through the 2000s for salmon but declined after the 1970s and 1980s for rockfishes, flatfishes, and crabs. Our results suggest that the spatial extent of recreational bottomfishing increased after the 1960s, when the availability of motorized vessels and advanced fish-finding technologies allowed anglers to expand their scope beyond localized angling from piers and boathouses. Respondents offered a wide range of reasons for shifts in fishing areas over time, reflecting substantial individual variation in motivations and behaviors. Changes in fishing areas were most commonly attributed to changes in residence and declines in target species and least tied to fishery regulations, despite the implementation of at least 25 marine preserves since 1970.

The reduction rates of DEPC-modified mutant Thermus thermophilus Rieske proteins differ when there is a negative charge proximal to the cluster.

Rieske and Rieske-type proteins are electron transport proteins involved in key biological processes such as respiration, photosynthesis, and detoxification. They have a [2Fe-2S] cluster ligated by two cysteines and two histidines. A series of mutations, L135E, L135R, L135A, and Y158F, of the Rieske protein from Thermus thermophilus has been produced which probe the effects of the neighboring residues, in the second sphere, on the dynamics of cluster reduction and the reactivity of the ligating histidines. These properties were probed using titrations and modifications with diethyl pyrocarbonate (DEPC) at various pH values monitored using UV-Visible and circular dichroism spectrophotometry. These results, along with results from EPR studies, provide information on ligating histidine modification and rate of reduction of each of the mutant proteins. L135R, L135A, and Y158F react with DEPC similarly to wild type, resulting in modified protein with a reduced [2Fe-2S] cluster in <90 min, whereas L135E requires >15 h under the same conditions. Thus, the negative charge slows down the rate of reduction and provides an explanation as to why negatively charged residues are rarely, if ever, found in the equivalent position of other Rieske and Rieske-type proteins.