Neonatal exposure to bisphenol analogues disrupts genital development in male mice.

The public concern and governmental regulations on bisphenol A (BPA) have stimulated the development and production of alternative analogues to replace BPA in a myriad of applications. Given the endocrine disrupting activities of BPA and potentially other analogues, the present study investigated and compared the effects of neonatal exposure to BPA, BPB, BPE, BPF, and BPS on the genital development in male mice. Pups were injected subcutaneously on the right shoulder in the mornings of postnatal days P0.5, P2, P4, and P6, resulting in a low dose of 0.05 µg/g body weight (bw)/day and a high dose of 10 µg/g bw/day. Mice were sacrificed at predetermined time and evaluated for gene expression levels (3 days after birth or P3), steroid hormone levels (P5), and morphological changes (P21). The results demonstrated that BPA, BPB, BPE, or BPF significantly shortened glans penis length and anogenital distance, while BPS didn't. Testis weight and anogenital distance were also significantly affected by BPA, BPE or BPF. The results also revealed that bisphenol analogues exposure significantly reduced testosterone levels, and altered the expression levels of developmental genes networks in developing penis of mice. Our data demonstrate that selected bisphenol analogues may possess similar endocrine disrupting effects compared to BPA, and exposure to these analogues could affect reproductive development of male mice. This raises the concern on the environmental and health safety of bisphenol analogues applied as industrial BPA replacements.

Statewide surveillance of halogenated flame retardants in fish in Illinois, USA.

In order to better understand the exposure of aquatic systems to halogenated flame retardant contaminants, the present study investigated a variety of legacy and emerging flame retardants in common carp and largemouth bass collected from 58 stations across Illinois (United States). The data revealed that polybrominated diphenyl ethers (PBDEs) generally dominated the flame retardant residues in Illinois fish. Concentrations of ΣPBDEs (including all detectable PBDE congeners) ranged from 24.7 to 8270 ng/g lipid weight (median: 135 ng/g lw) in common carp and 15-3870 ng/g lw (median: 360 ng/g lw) in largemouth bass. In addition to PBDEs, Dechlorane analogues (i.e. Dec-603, Dec-604, and Chlordane Plus) were also frequently detected. Median concentrations of ΣDechloranes (including all detected Dechlorane analogues) were 34.4 and 23.3 ng/g lw in common carp and largemouth bass, respectively. Other emerging flame retardants, including tetrabromo-o-chlorotoluene (TBCT), hexabromobenzene (HBBZ), 2-ethylhexyltetrabromobenzoate (EH-TBB), and bis(2-ethylhexyl)-3,4,5,6-tetrabromo-phthalate (BEH-TEBP), were also detected in 40-78% of the fish at the monitored stations. Spatial analysis revealed significantly greater PBDE concentrations in fish living in impaired urban streams and lakes compared to those from the impaired agricultural and unimpaired agricultural/urban waters, demonstrating a significant urban influence on PBDE contamination. Future studies and environmental monitoring are recommended to focus on temporal trends of PBDEs and alternative flame retardants, as well as human exposure risks via edible fishes, in the identified Areas of Concern within Illinois.

Bisphenol Analogues Other Than BPA: Environmental Occurrence, Human Exposure, and Toxicity-A Review.

Numerous studies have investigated the environmental occurrence, human exposure, and toxicity of bisphenol A (BPA). Following stringent regulations on the production and usage of BPA, several bisphenol analogues have been produced as a replacement for BPA in various applications. The present review outlines the current state of knowledge on the occurrence of bisphenol analogues (other than BPA) in the environment, consumer products and foodstuffs, human exposure and biomonitoring, and toxicity. Whereas BPA was still the major bisphenol analogue found in most environmental monitoring studies, BPF and BPS were also frequently detected. Elevated concentrations of BPAF, BPF, and BPS (i.e., similar to or greater than that of BPA) have been reported in the abiotic environment and human urine from some regions. Many analogues exhibit endocrine disrupting effects, cytotoxicity, genotoxicity, reproductive toxicity, dioxin-like effects, and neurotoxicity in laboratory studies. BPAF, BPB, BPF, and BPS have been shown to exhibit estrogenic and/or antiandrogenic activities similar to or even greater than that of BPA. Knowledge gaps and research needs have been identified, which include the elucidation of environmental occurrences, persistence, and fate of bisphenol analogues (other than BPA), sources and pathways for human exposure, effects on reproductive systems and the mammary gland, mechanisms of toxicity from coexposure to multiple analogues, metabolic pathways and products, and the impact of metabolic modification on toxicity.