Effect of infant formuLas on blood and tissue cholesterol, bone calcium, and body composition in weanling pigs.

Weanling pigs were fed four commercial infant formulas to determine effects on blood and tissue cholesterol parameters and on body composition. Two milk protein (MP)-based formulas and two soy protein isolate (SPI)-based isocaloric formulas were fed in concentrated liquid form for 32 days. A commercial sow's milk replacer fed to a fifty group of pigs served as a control diet. Pigs fed SPI-based formula had significantly less cholesterol in the plasma than did pigs fed MP-based formula. Whole-body and adipose tissue cholesterol concentrations were greatest in pigs fed formulas containing a relatively high concentration of polyunsaturated fatty acids (PUFA). Liver cholesterol concentration was inversely related to plasma cholesterol concentrations. Bone calcium, measured as percentage of dry, fat-free bone (femur), and whole-carcass ash were significantly less in pigs fed SPI-based formula than in pigs fed MP-based formula. Similar growth and development were observed in pigs fed SPI-based or MP-based formulas; however, pigs fed SPI-based formulas had significantly less bone calcium.

Blood plasma lipoprotein and tissue cholesterol of calves fed soybean oil, corn oil, vegetable shortening or tallow.

The objective of this study was to determine cholesterol content of blood plasma, blood plasma lipoproteins and tissues of calves fed fats of differing compositions. Groups of 2-week-old calves were fed one of the following fats in a reconstituted milk formula: soybean oil, corn oil, vegetable shortening or tallow. The diets contained no dry feed or added cholesterol. Blood plasma cholesterol concentrations increased with time for all groups. After 15 weeks, cholesterol concentrations were greater in the blood, liver and fat of the groups fed soybean oil and corn oil than in those of the groups fed vegetable shortening and tallow. Low density lipoprotein was identified as the carrier of the increased amounts of cholesterol noted in the blood.

Effect of various triglycerides on blood and tissue cholesterol of calves.

Blood and tissue cholesterol responses were measured in six groups of four calves each fed for 24 weeks reconstituted nonfat dry fat milk in which 30% of calories was derived from one of the following: soybean oil (SBO), beef tallow (T), medium-chain triglycerides (MCT), and 1:1 soybean-oil tallow (SBO/T) combination. Two groups of four calves each also were fed SBO-milk plus dry feed and T-milk plus dry feed, respectively. Blood cholesterol concentration was significantly greater in SBO-fed calves than in T-fed calves. Calves fed MCT-milk had significantly lower blood cholesterol than calves fed T- or SBO-milk. Significant differences in cholesterol concentration were noted for the liver, perianal fat, and for omental fat.

Type and amount of dietary fat affect relative concentration of cholesterol in blood and other tissues of calves.

Sixteen male Holstein calves consumed by nipple a reconstituted milk containing nonfat dry milk and either soybean oil or tallow for 24 weeks. Calves fed milk in this manner (with no dry feed) are functionally nonruminants because the milk bypasses the ruminoreticulum. The fat in these diets contributed about 30% of total dietary calories. The calves consuming soybean oil milk had significantly greater amounts of cholesterol in blood plasma and adipose tissue than did the calves consuming tallow milk.

The effects of supplemental dietary cholesterol and exercise on blood cholesterol and atherosclerosis in the goat.

The objective of this experiment was to determine the effects of supplemental dietary cholesterol and treadmill exercise on blood plasma cholesterol and development of atherosclerosis in young goats. Eighteen two-week-old goats, assigned to four groups for 22 weeks, were fed 100 g whole milk and, after 14 weeks, 50 g corn and cob meal daily/kg body weight. The four groups received, respectively, 250, 175, 100 and 25 mg cholesterol/kg body weight daily in the milk. From week 10 to week 22 of the experiment half of the goats in each group were exercised on a motorized treadmill at a rate of 6.4 km/h for 15 min daily, five days per week. Addition of cholesterol to the diet increased the mean plasma cholesterol in each of the four groups, but between-group comparisons were statistically significant for only the 250-mg group versus the 25-mg group. Exercise had no effect on plasma cholesterol. Analysis of serial blood samples of 16 of the goats during one day of the experiment indicated no significant diurnal variations. Six males, three exercised and three nonexercised, were sacrificed; all had extensive aortic sudanophilia. Histological preparations from sudanophilic areas of all aortas showed areas of intimal thickening composed of foam cells. These same areas stained strongly for lipid with Oil Red 0. No histological evidence of calcium deposition or fibrous plaques was found.

Type and amount of dietary fat affect relative concentration of cholesterol in blood and other tissues of rats.

The effects of diet on tissue cholesterol disposition in the rat were studied. Growing rats were fed a nonfat dry milk supplemented with two levels of soy-bean oil (SBO) and tallow (T) such that either 30% or 50% of total dietary calories came from fat. Two of four groups of rats fed the diets with 50% of calories from fat were supplemented with 20% ground whole oats. Considering all diets, rats fed SBO had higher blood and kidney cholesterol than did rats fed T; supplementation of the diet with oats increased the plasma cholesterol of the "50%" SBO rats and , conversely, decreased plasma cholesterol of the "50%" T rats. Muscle cholesterol content was not affected by variations in dietary fat and oats. In all treatments, cholesterol concentration of epididymal fat and liver were greater in the SBO-fed than in the T-fed rats.

Milk and tissue lipid composition after feeding cows protected polyunsaturated fat for two years.

The long-term effects of feeding Holstein cows plant lipids protected from microbial hydrogenation in the rumen were studied. Of particular interest were cow health and changes in fatty acid and cholesterol concentrations of milk and meat. Safflower oil-casein or safflower oil-casein treated with formaldehyde to impede microbial attack were fed to two groups of three cows as 10% of the concentrate ration for two lactations. Production of milk fat of cows fed the protected concentrate increased significantly. Linoleic acid of milk fat was twice normal, providing a polyunsaturated milk. Cholesterol of milk or meat did not increase even though cholesterol of blood plasma was higher in both groups fed safflower oil than in control cows. Cardiovascular systems showed no marked abnormalities and no differences that could be due to treatment. All cows maintained normal health and milk production throughout the experiment.

Changes in receptor sensitivity of the cerebral cortex and liver during chronic ethanol ingestion and withdrawal.

The evidence was reviewed supporting the noradrenergic sub- and supersensitivity hypothesis of ethanol withdrawal pathogenesis. New data indicates that the cAMP generating system linked to noradrenergic receptor sensitivity is in a steady state in the brain during ethanol withdrawal since the cAMP levels were not different from controls in vivo. Propranolol blocking experiments indicated the beta-adrenergic receptor is a necessary link in the increased cAMP response induced by ethanol withdrawal. The changes in sensitivity observed in the ethanol withdrawn rats were reproduced by acute and chronic reserpinization.

Prevention of parturient paresis by a low-calcium diet prepartum: a field study.

Twenty of 60 control cows fed a "normal" diet prepartum developed parturient paresis whereas none of 37 fed a low-calcium (13 to 18 g/cow per day) diet prepartum developed the disease.