Reproductive Performance of Ewes, Fed Flushing Diet at Different Management Feeding Program.

<b>Background and Objective:</b> There is very rare information regarding data of reproduction performance of small ruminant with different management flushing program in Asian countries. The aim of this study was to evaluate the implementation of flushing diet program containing lemuru fish oil with time management difference, by evaluating the reproductive performance of local Garut ewes. <b>Materials and Methods:</b> Twenty of garut ewes (average body weight 30.06±6.20 kg) were used in this experiment using Completely Randomized Block Design (CRBD), in four treatments with five animals of each treatment. The treatments were without flushing (F0 as control), flushing at the beginning of mating (F1 = 2 weeks pre-mating until 2 weeks just after-mating), two times flushing (F2 = F1 plus 4 weeks flushing during mid gestation) and three times flushing (F3 = F2 plus 2 weeks flushing at the end of gestation and 2 weeks after parturition). The ratio of flushing or basal concentrate to forage was 70:30. Basal concentrate was given during untreated. Nutrients consumption, body condition score (BCS), average daily gain (ADG), feed efficiency ratio (FER) and reproduction parameters were evaluated. The data of sex ratio and birth type were expressed descriptively. <b>Results:</b> The results showed that the treatment had significant effect (p<0.05) on crude fat consumption, total digestible nutrients (TDN) and changes of BCS values, but had no effect on other nutrients consumptions. All treatments did not affect to total birth weight, the number of embryo, litter size, gestation period and sex ratio. The percentage of ewes pregnancy with the flushing treatment was 25% higher than the control. The flushing treatments were significantly effect on partus weight of ewes (p<0.05). <b>Conclusion:</b> It was concluded that three times flushing program at early, middle and late of gestation could improve the nutritional status and reproductive performance of ewes, such as fat intake, partus weight, improved percentage of pregnancy and type of birth, but did not affect to total birth weight, litter size, pregnant period and sex ratio.

Dependence of alignment direction on magnitude of strain in esophageal smooth muscle cells.

The response of cells in vitro to mechanical forces has been the subject of much research using devices to exert controlled mechanical stimulation on cultured cells or isolated tissue. In this study, esophageal smooth muscle cells were seeded on flexible polyurethane membranes to form a confluent cell layer. The cells were then subjected to uniform cyclic stretch of varying magnitudes at a frequency of approximately five cycles per minute in a custom made mechatronic bioreactor, providing similar strains experienced in the in vivo mechanical environment of the esophagus. The results show that the orientation response is dependent on the magnitude of cyclic stretch applied. Smooth muscle cells showed parallel alignment to the force direction at low cyclic strains (2%) compared to the hill-valley morphology of static controls. At higher strains (5% and 10% magnitude), the cells exhibited a consistent alignment perpendicular to the strain. To our knowledge, this is the first time that the alignment direction's dependence on strain magnitude has been demonstrated. MTS analysis indicated that cell metabolism was reduced when mechanical strain was applied, and proliferation was inhibited by mechanical strain. Protein expression indicates a decrease in smooth muscle alpha-actin, indicative of changes in cell phenotype, an increase in vimentin, which is associated with increased cell motility, and an increase in desmin, indicating differentiation in stimulated cells.